Abstract
Background
Households are hot spots for severe acute respiratory syndrome coronavirus 2 transmission.
Methods
This prospective study enrolled 100 coronavirus disease 2019 (COVID-19) cases ...and 208 of their household members in North Carolina though October 2020, including 44% who identified as Hispanic or non-White. Households were enrolled a median of 6 days from symptom onset in the index case. Incident secondary cases within the household were detected using quantitative polymerase chain reaction of weekly nasal swabs (days 7, 14, 21) or by seroconversion at day 28.
Results
Excluding 73 household contacts who were PCR-positive at baseline, the secondary attack rate (SAR) among household contacts was 32% (33 of 103; 95% confidence interval CI, 22%–44%). The majority of cases occurred by day 7, with later cases confirmed as household-acquired by viral sequencing. Infected persons in the same household had similar nasopharyngeal viral loads (intraclass correlation coefficient = 0.45; 95% CI, .23–.62). Households with secondary transmission had index cases with a median viral load that was 1.4 log10 higher than those without transmission (P = .03), as well as higher living density (more than 3 persons occupying fewer than 6 rooms; odds ratio, 3.3; 95% CI, 1.02–10.9). Minority households were more likely to experience high living density and had a higher risk of incident infection than did White households (SAR, 51% vs 19%; P = .01).
Conclusions
Household crowding in the context of high-inoculum infections may amplify the spread of COVID-19, potentially contributing to disproportionate impact on communities of color.
Households are hot spots for severe acute respiratory syndrome coronavirus 2 transmission. In the United States, household crowding in the context of high-inoculum infections may amplify the spread of COVID-19, potentially contributing to disproportionate impact on communities of color.
Autism spectrum disorder (ASD) is a neurodevelopmental condition impacting individuals’ social communication and interactions. Animal-assisted interventions (AAI) have been identified as practice ...modalities for children with ASD. A systematic review of the literature was completed identifying 13 articles addressing the impact AAI has on the social behaviours of children with ASD. Participant numbers were small with ages ranging from 3 to 18 years. Outcomes comprised verbal communication, non-verbal communication, identified desired and undesired behaviours. Findings suggested that AAI can have a positive impact on the social behaviours of children on the autism spectrum; however, studies were characterised by methodological weaknesses. More rigorous research methods are required to determine the effectiveness of AAI for children with ASD.
Abstract
Body temperature (BT) is a reliable method for evaluating the thermal status of cattle. The objective of this study was to evaluate the regulation of BT in B. indicus and B. taurus steers ...during a heat wave event. Thirty-five steers (466.30±10.2kg) of mixed genotypes (B. taurus, n = 18; B. indicus, n = 17) were used in a larger 100-d study. Cattle were housed in six un-shaded pens at a commercial feedlot. Body temperature data for this were obtained at 1 h intervals over a 3-d heat wave event using in situ abdominal data loggers. Data were analysed using a repeated measures model, using residual maximum likelihood estimation. The model included genotype (B. taurus; B. indicus) and animal ID as random effects and hour as a fixed effect. Mean maximum BT occurred at 1500 h for B. taurus (40.52±0.03ºC) and 0400 h for B. indicus (40.48±0.31ºC). The BT of B. indicus decreased between 0400 h and 0700 h, and then increased to 1100 h (+0.10±0.01ºC/h), before decreasing again. The BT of B. taurus decreased between 0500 h and 0600 h, and then slowly increased (+0.04±0.01ºC/h) to 1500 h. Mean minimum BT occurred at 2100 h for B. taurus (40.12±0.11ºC) and 0700 h for B. indicus (40.00±0.22ºC). B. taurus maintained lower BT (40.21±0.05ºC) between 1900 h and 2200 h compared to B. indicus (40.27±0.05ºC) which may be due to disrupted heat loss mechanisms during the day, highlighting the importance of night time cooling. In response to increasing heat load, BT in B. taurus had slower increases but retained heat for longer, whilst B. indicus BT responded by a series of rapid increases and decreases. Developing an understanding of the differences in BT regulation in B. indicus and B. taurus will allow for more effective heat load management strategies during heat waves to be established.
Abstract
Body temperature (BT) is a reliable method for evaluating the thermal status of cattle. The objective of this study was to evaluate the regulation of BT in B. indicus and B. taurus steers ...during a heat wave event. Thirty-five steers (466.30±10.2kg) of mixed genotypes (B. taurus, n = 18; B. indicus, n = 17) were used in a larger 100-d study. Cattle were housed in six un-shaded pens at a commercial feedlot. Body temperature data for this were obtained at 1 h intervals over a 3-d heat wave event using in situ abdominal data loggers. Data were analysed using a repeated measures model, using residual maximum likelihood estimation. The model included genotype (B. taurus; B. indicus) and animal ID as random effects and hour as a fixed effect. Mean maximum BT occurred at 1500 h for B. taurus (40.52±0.03ºC) and 0400 h for B. indicus (40.48±0.31ºC). The BT of B. indicus decreased between 0400 h and 0700 h, and then increased to 1100 h (+0.10±0.01ºC/h), before decreasing again. The BT of B. taurus decreased between 0500 h and 0600 h, and then slowly increased (+0.04±0.01ºC/h) to 1500 h. Mean minimum BT occurred at 2100 h for B. taurus (40.12±0.11ºC) and 0700 h for B. indicus (40.00±0.22ºC). B. taurus maintained lower BT (40.21±0.05ºC) between 1900 h and 2200 h compared to B. indicus (40.27±0.05ºC) which may be due to disrupted heat loss mechanisms during the day, highlighting the importance of night time cooling. In response to increasing heat load, BT in B. taurus had slower increases but retained heat for longer, whilst B. indicus BT responded by a series of rapid increases and decreases. Developing an understanding of the differences in BT regulation in B. indicus and B. taurus will allow for more effective heat load management strategies during heat waves to be established.
Since the early 2000s routine fish surveys have recorded increasing numbers of snake pipefish, Entelurus aequoreus, in the northeast Atlantic. Fishermen and divers have also commented on this ...increase and pipefish have started to appear in the diet of seabirds and other marine predators. This paper collates information from these diverse sources and assesses the current status of snake pipefish. We found compelling evidence of a dramatic increase in the abundance of snake pipefish starting around 2003 and continuing up to the present (2006) and a range expansion northwards to Spitzbergen and the Barents Sea. Since 2004 snake pipefish have been increasingly recorded in the diet of many species of seabird breeding in colonies around the coast of the UK, and in Norway, Iceland and the Faeroe Islands. Information on the nutrient value of snake pipefish is currently lacking but their rigid, bony structure makes them difficult for young seabirds to swallow and there are numerous records of chicks choking to death. Thus, in the case of avian predators during the breeding season, it appears unlikely that increased abundance of snake pipefish will provide a useful alternative prey. The reason for the rapid and dramatic increase in numbers of snake pipefish is currently unclear but such events are characteristic of marine ecosystems and will almost certainly have an effect on food web dynamics. PUBLICATION ABSTRACT
ABSTRACT
Introduction
The aim of this observational pilot study was to assess the safety, feasibility, preliminary outcomes, and predictors of participant response as a result of implementing an ...equine-assisted intervention within a residential substance abuse treatment program at a large Veterans Administration medical center. A secondary aim was to evaluate psychological instruments for use in future, more rigorous studies. The overarching goal was to complete the necessary work to prepare for a large randomized controlled trial of this intervention for Veterans with addictive disorders.
Materials and Methods
Participants were 33 Veterans, 29 males and 4 females, who participated in one 4-hour session of combined equine-assisted learning and equine-assisted psychotherapy during an admission to a residential substance abuse treatment program. Preintervention and postintervention instruments were utilized to assess changes in affect, anxiety, and craving. Demographic and diagnostic variables were evaluated for the potential to predict outcomes.
Results
The intervention was safe and feasible to utilize as there were no adverse outcomes to patients, staff, or equines. The State-Trait Anxiety Inventory, Craving Experience Questionnaire, and Positive and Negative Affect Scale revealed preliminary findings of significant preintervention to postintervention decreases in anxiety, negative affect, and craving, as well as increased positive affect. Lastly, the presence or absence of a history of suicide attempts and/or suicidal ideation were predictive of some postintervention scores.
Conclusion
While more rigorous studies are needed, these results indicate that the intervention evaluated in this study is safe and feasible to utilize for Veterans admitted to a residential substance abuse treatment program. Furthermore, preliminary outcomes suggest that this intervention, and perhaps other equine-assisted interventions, has the potential to be beneficial to Veterans with addictive disorders as well as those at risk of suicide. The psychological instruments used in this intervention appear to be appropriate for use in future investigations. Additionally, more rigorous studies are warranted, and this work provides the necessary first steps needed to proceed with those investigations.
Background & Aims: Infectious diarrhea caused by viruses plus enterotoxigenic bacteria is often more severe than diarrhea induced by either pathogen alone. We postulated that the increased cell ...adenosine 3',5'-cyclic monophosphate (cAMP) concentration observed during infection by enterotoxigenic organisms retards the intestinal repair process by blocking activation of mitogen-activated protein kinases (MAPKs) in proliferating intestinal cells.
Methods: We evaluated the effects of glutamine on MAPK activity, thymidine incorporation, and cell number in glutamine-starved and -sufficient rat intestinal crypt cells (IEC-6).
Results: In glutamine-starved cells, 10 mmol/L glutamine in the absence of serum stimulated
3Hthymidine incorporation 8-fold. This effect was inhibited by 60% with 8-(4-chlorophenylthio) (8-CPT)-cAMP (100 μmol/L) + isobutyl methylxanthine (100 μmol/L). In cells not starved of glutamine, glutamine stimulated thymidine incorporation by 3-fold, and 8-CPT-cAMP completely blocked the mitogenic effect. Inhibition of proliferation by cAMP persisted for at least 68 hours after cAMP removal. In vitro kinase assays showed that glutamine signaling requires an intact ERK (extracellular signal–related kinase) pathway in unstarved cells. In starved cells, at least one other pathway (JNK) was activated by glutamine, and the mitogenic inhibition by 8-CPT-cAMP was incomplete. Other intestinal fuels (glucose and acetate) were not mitogenic.
Conclusions: Increased levels of intracellular cAMP inhibit ERKs but only partially reduce glutamine-stimulated proliferation in enterocytes adapted to low glutamine.
GASTROENTEROLOGY 2000;118:90-100
The aim of this observational pilot study was to assess the safety, feasibility, preliminary outcomes, and predictors of participant response as a result of implementing an equine-assisted ...intervention within a residential substance abuse treatment program at a large Veterans Administration medical center. A secondary aim was to evaluate psychological instruments for use in future, more rigorous studies. The overarching goal was to complete the necessary work to prepare for a large randomized controlled trial of this intervention for Veterans with addictive disorders.
Participants were 33 Veterans, 29 males and 4 females, who participated in one 4-hour session of combined equine-assisted learning and equine-assisted psychotherapy during an admission to a residential substance abuse treatment program. Preintervention and postintervention instruments were utilized to assess changes in affect, anxiety, and craving. Demographic and diagnostic variables were evaluated for the potential to predict outcomes.
The intervention was safe and feasible to utilize as there were no adverse outcomes to patients, staff, or equines. The State-Trait Anxiety Inventory, Craving Experience Questionnaire, and Positive and Negative Affect Scale revealed preliminary findings of significant preintervention to postintervention decreases in anxiety, negative affect, and craving, as well as increased positive affect. Lastly, the presence or absence of a history of suicide attempts and/or suicidal ideation were predictive of some postintervention scores.
While more rigorous studies are needed, these results indicate that the intervention evaluated in this study is safe and feasible to utilize for Veterans admitted to a residential substance abuse treatment program. Furthermore, preliminary outcomes suggest that this intervention, and perhaps other equine-assisted interventions, has the potential to be beneficial to Veterans with addictive disorders as well as those at risk of suicide. The psychological instruments used in this intervention appear to be appropriate for use in future investigations. Additionally, more rigorous studies are warranted, and this work provides the necessary first steps needed to proceed with those investigations.
To explore trends in intraoperative procoagulant factor concentrate use in patients undergoing heart transplantation (HTx) in Virginia. Secondarily, to evaluate their association with postoperative ...thrombosis.
Patients who underwent HTx were identified using a statewide database. Trends in off-label recombinant activated factor VII (rFVIIa) use and on-label and off-label prothrombin complex concentrate (PCC) use were tested using the Mantel-Haenszel test. Multivariate logistic regression was used to test for an association between procoagulant factor concentrate administration and thrombosis.
Virginia hospitals performing HTx.
Adults undergoing HTx between 2012 and 2022.
None.
Among 899 patients who required HTx, 100 (11.1%) received off-label rFVIIa, 69 (7.7%) received on-label PCC, and 80 (8.9%) received off-label PCC. There was a downward trend in the use of rFVIIa over the 10-year period (p = 0.04). There was no trend in on-label PCC use (p = 0.12); however, there was an increase in off-label PCC use (p < 0.001). Patients who received rFVIIa were transfused more and had longer cardiopulmonary bypass time (p < 0.001). Receipt of rFVIIa was associated with increased thrombotic risk (odds ratio OR 1.92; 95% CI 1.12-3.29; p = 0.02), whereas on-label and off-label PCC use had no association with thrombosis (OR 0.98, 95% CI 0.49-1.96, p = 0.96 for on-label use; and OR 0.61, 95% CI 0.29-1.30, p = 0.20 for off-label use).
Use of rFVIIa in HTx decreased over the past decade, whereas off-label PCC use increased. Receipt of rFVIIa was associated with thrombosis; however, patients who received rFVIIa were more severely ill, and risk adjustment may have been incomplete.
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