In view of a simple after-use separation, the potentiality of producing magnetic activated carbon (MAC) by intercalation of ferromagnetic metal oxide nanoparticles in the framework of a powder ...activated carbon (PAC) produced from primary paper sludge was explored in this work. The synthesis conditions to produce cost effective and efficient MACs for the adsorptive removal of pharmaceuticals (amoxicillin, carbamazepine, and diclofenac) from aqueous media were evaluated. For this purpose, a fractional factorial design (FFD) was applied to assess the effect of the most significant variables (Fe
to Fe
salts ratio, PAC to iron salts ratio, temperature, and pH), on the following responses concerning the resulting MACs: Specific surface area (
), saturation magnetization (
), and adsorption percentage of amoxicillin, carbamazepine, and diclofenac. The statistical analysis revealed that the PAC to iron salts mass ratio was the main factor affecting the considered responses. A quadratic linear regression model A = f(
,
) was adjusted to the FFD data, allowing to differentiate four of the eighteen MACs produced. These MACs were distinguished by being easily recovered from aqueous phase using a permanent magnet (
of 22-27 emu g
), and their high
(741-795 m
g
) were responsible for individual adsorption percentages ranging between 61% and 84% using small MAC doses (35 mg L
).
Electrocution can pose a serious threat to large birds, particularly threatened species with low population sizes. However, few studies have focused on the impacts of electrocution on large parrots ...such as the Endangered Lear's Macaw Anodorhynchus leari, endemic to the Brazilian Caatinga. Here, we compile and describe 31 electrocution events, as reported by villagers, indicating that electrocution may be an important threat to Lear's Macaw. We suggest a research and monitoring agenda to better understand the spatial and temporal patterns of this impact and recommend some immediate mitigation measures for decreasing electrocutions.
Eletrocussões podem ser uma importante ameaça para aves de grande porte, principalmente para espécies ameaçadas em virtude do seu reduzido tamanho populacional. Poucos estudos têm como foco o efeito das eletrocussões de psitacídeos, em particular com grandes espécies como a arara‐azul‐de‐lear, endêmica da Caatinga e ameaçada de extinção. A partir de relatos e evidências dos moradores locais, nós compilamos e descrevemos 31 registros de eletrocussão contabilizados até junho de 2021, indicando que esta pode ser uma nova e importante ameaça para a arara‐azul‐de‐lear. Nós sugerimos uma agenda de pesquisa e monitoramento para incrementar a compreensão sobre os padrões temporais e espaciais deste impacto e também recomendamos ações de mitigação com o potencial de reduzir a ocorrência de eletrocussões nesta espécie e em outros psitacídeos.
Purpose
Pseudoxanthoma elasticum (PXE) is a recessive disorder involving skin, eyes and arteries, mainly caused by ABCC6 pathogenic variants. However, almost one fifth of patients remain genetically ...unsolved despite extensive genetic screening of ABCC6, as illustrated in a large French PXE series of 220 cases. We searched for new PXE gene(s) to solve the ABCC6‐negative patients.
Methods
First, family‐based exome sequencing was performed, in one ABCC6‐negative PXE patient with additional neurological features, and her relatives. CYP2U1, involved in hereditary spastic paraplegia type 56 (SPG56), was selected based on this complex phenotype, and the presence of two candidate variants. Second, CYP2U1 sequencing was performed in a retrospective series of 46 additional ABCC6‐negative PXE probands. Third, six additional SPG56 patients were evaluated for PXE skin and eye phenotype. Additionally, plasma pyrophosphate dosage and functional analyses were performed in some of these patients.
Results
6.4% of ABCC6‐negative PXE patients (n = 3) harboured biallelic pathogenic variants in CYP2U1. PXE skin lesions with histological confirmation, eye lesions including maculopathy or angioid streaks, and various neurological symptoms were present. CYP2U1 missense variants were confirmed to impair protein function. Plasma pyrophosphate levels were normal. Two SPG56 patients (33%) presented some phenotypic overlap with PXE.
Conclusion
CYP2U1 pathogenic variants are found in unsolved PXE patients with neurological findings, including spastic paraplegia, expanding the SPG56 phenotype and highlighting its overlap with PXE. The pathophysiology of ABCC6 and CYP2U1 should be explored to explain their respective role and potential interaction in ectopic mineralization.
Transparent conductive oxides, such as aluminum-doped zinc oxide (AZO), are of substantial importance for use in a broad range of applications because of their excellent optical and electrical ...properties. AZO film can be deposited by using several conventional techniques, although they suffer from limitations such as long deposition time, high cost, and the requirement for complex deposition equipment. Here, we used hollow-cathode glow discharge, which produces a high-density plasma and achieves high deposition efficiency. Remarkably, instead of metallic target materials, we used Al
2
O
3
and ZnO powders filled in the hollow cathode as a target, thus avoiding the need for specifically designed targets in this technique. The films were deposited using mixtures of argon and oxygen at various ratios (0% to 50% oxygen), to improve the film characteristics. The films deposited under all conditions exhibited hexagonal wurtzite ZnO structure, while the grain size increased with increasing oxygen content. The film was thick and porous when using low oxygen content, but became thin and dense with increasing oxygen content. The optical transmittance was found to be strongly dependent on the processing gases used, with the highest transmittance of 84% being attained when using 25% oxygen gas. The bandgap of the films lay between 3.27 eV and 3.33 eV. The highest carrier concentration and mobility were attained when using 25% oxygen, and the Hall resistivity decreased with increasing oxygen content. Besides the excellent transmittance and electrical properties of the deposited films, it is expected that the results of this study will be useful for solar cells and optoelectronic applications due to the relatively low cost of this technique and the lack of specific target requirements.
•Study of distinct cork agglomerates, with different densities and granule sizes.•Static and dynamic (impact) tests compare and fully characterize the materials.•The capacity of the different ...compounds to withstand multiple impacts is studied.•Allow proper material choices using this fully sustainable and recyclable material.
Cork is a natural cellular material capable of withstanding considerable amounts of energy and exhibiting a viscoelastic return to its original shape. This feature is particularly interesting to resist to successive impacts. In this study, the behavior of different types of agglomerated cork (AC) and expanded cork (EC) is investigated under static and dynamic loadings. Double impact was carried out on the samples using a hemispheric actuator. The peak acceleration data for all compounds were further analyzed. Static compression tests gave an interesting insight into the stress–strain curve of agglomerates and Poisson’s ratio variation during deformation. Results demonstrate a clear influence of agglomerated density and grain size on the resulting mechanical properties and point out a tremendous potential for this sustainable material to be tailored to fit diverse crashworthiness applications.
Large volumes of produced water are generated as a byproduct in activities of oil and gas exploitation, which can be reused in agriculture after a treatment process. Activated sludge treatment has ...been successfully used to remove oil from wastewater, but systematic studies on the toxicity of this effluent using this treatment are scarce in the literature. In this study, it was investigated the performance of an activated sludge system in the treatment of a synthetic produced water under different initial conditions in terms of salinity and oil and grease concentration. Furthermore, it was evaluated this effluent phytotoxicity in the germination, and seedling and plant growths of sunflower and corn seeds using untreated and treated synthetic produced water. Results revealed the activated sludge effectiveness in oil and grease and salinity removal from produced water, viz. high removal efficiency of 99.01 ± 0.28 and 91.07 ± 0.39%., respectively. Untreated produced water showed considerable toxic effects on the germination (74.67 ± 2.31% and 82.67 ± 2.31 for sunflower and corn seeds, respectively) and growth stages of sunflower and corn seed plants. The germination percentage was approximately 100% for both types of seed. The seedling and plant growth of the two seeds irrigated with treated produced water had similar performance when used tap water. These results highlighted the potential reuse as an unconventional water resource for plant irrigation of the synthetic produced water treated by an activated sludge process, which technology has showed high removal performance of salinity and oil.
Over the last decade, there has been a dramatic increase in the prevalence and gravity of systemic fungal diseases. This study aimed therefore at evaluating the antifungal potential of ester ...derivatives of benzoic and cinnamic acids from three Candida species. The compounds were prepared via Fischer esterification, and the antifungal assay was performed by the microdilution method in 96-well microplates for determining the minimal inhibitory concentrations (MICs). The findings of the antifungal tests revealed that the analogue compound methyl ferulate, methyl o-coumarate, and methyl biphenyl-3-carboxylate displayed an interesting antifungal activity against all Candida strains tested, with MIC values of 31.25-62.5, 62.5-125, and 62.5 μg/ml, respectively. A preliminary Structure-Activity Relationship study of benzoic and cinnamic acid derivatives has led to the recognition of some important structural requirements for antifungal activity. The results of molecular docking indicate that the presence of the enoate moiety along with hydroxyl and one methoxy substitution in the phenyl ring has a positive effect on the bioactivity of compound 7 against Candida albicans. These observations further support the hypothesis that the antifungal activity of compound 7 could be due to its binding to multiple targets, specifically to QR, TS, and ST-PK. Additional experiments are required in the future to test this hypothesis and to propose novel compounds with improved antifungal activity.
Scaffolds based on aligned and non-aligned poly (L-lactic acid) (PLLA)/polycaprolactone (PCL) fibers obtained by electrospinning, associated to electrosprayed hydroxyapatite (HA) for tissue ...engineering applications were developed and their performance was compared in terms of their morphology and biological and mechanical behaviors. The morphological results assessed by scanning electron microscopy showed a mesh of PLLA/PCL fibers (random and perfectly aligned) associated with aggregates of nanophased HA. Fourier transform infrared spectrometry confirmed the homogeneity in the blends and the presence of nanoHA in the scaffold. As a result of fiber alignment a 15-fold increase in Young's Modulus and an 8-fold increase in tensile strength were observed when compared to non-aligned fibers. In PLLA/PCL/HA scaffolds, the introduction of nanoHA caused a remarkable improvement of the mechanical strength of this material acting as a reinforcement, enhancing the response of these constructs to tensile stress. In vitro testing was evaluated using osteoblast (MC3T3-E1) cells. The results showed that both fibrous scaffolds were able to support osteoblast cell adhesion and proliferation and that fiber alignment induced increased cellular metabolic activity. In addition, the adhesion and proliferation of
were evaluated and a lower number of colony forming units (CFUs) was obtained in the scaffolds with aligned fibers.
A growing number of studies have found that the gut microbiota is involved in a variety of psychological processes and neuropsychiatric disorders, which include mood and anxiety disorders. ...Consumption of dairy products may contain bioactive compounds and probiotic bacteria with various therapeutic benefits. The aim of the study was to investigate possible associations between the frequency of consumption of different types of dairy products and the state of anxiety in university students. The subjects were 311 Azorean university students, 231 women and 80 men, with an average age of 20.5 years. Subjects completed a quantitative questionnaire on the frequency of dairy product consumption and a short version of the Spielberger State-Trait Anxiety Inventory (STAI) test. Among dairy products, semi-skimmed milk was the most commonly consumed, followed by cheese (ripened), drinking yogurt, skim milk, and set yogurt, while fresh cheese, whole milk, and dairy ice cream were the least common. Discriminant analysis showed that consumption of fermented products (yogurt and cheese) was significantly higher (
P
< 0.05) in the group with low anxiety level (score <40 in STAI test) than in the group with higher anxiety level (score ≥ 40). In this analysis, 62.4% of the initially grouped cases were correctly classified according to the frequency of fermented products consumption. No correlations were found between anxiety and unfermented dairy products. The results indicate that the consumption of fermented dairy products has a positive effect on reducing anxiety in young Azorean university students.
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