Species and generic recognition in the order
Xylariales
has been uncertain due to lack of molecular data from authentic cultures, as well as overlapping morphological characteristics. In this study, ...we revise the families
Graphostromataceae
,
Hypoxylaceae
,
Lopadostomataceae
and
Xylariaceae
in
Xylariales
. Our study is based on DNA sequence data derived from living cultures of fresh isolates, data from GenBank and morphological observation of type and worldwide herbarium specimens. We also collected new specimens from Germany, Italy and Thailand. Combined analyses of ITS, LSU, RPB2 and β-tubulin sequence data were used to reconstruct the molecular phylogeny of the above families. Generic and familiar boundaries between these families are revised and presented in an updated combined phylogenetic tree. We accept six genera in
Graphostromataceae
, 19 genera in
Hypoxylaceae
, four in
Lopadostomataceae
and 37 genera in
Xylariaceae
. Five genera previously treated in
Xylariaceae
are placed in
Amphisphaeriales
genera
incertae sedis
and seven genera are placed in
Xylariales
genera
incertae sedis.
Two genera are placed in Sordariomycetes genera
incertae sedis
, while four genera are placed as Xylariomycetidae genera
incertae sedis.
Three genera are considered as doubtful.
Barrmaelia
and
Cannonia
, presently included in
Xylariaceae
are transferred to
Diatrypaceae
and
Coniochaetales
respectively, based on their morphology and phylogeny.
Areolospora
and
Myconeesia
are excluded from
Xylariaceae
and synonymized with
Phaeosporis
and
Anthostomella
respectively. Updated descriptions and illustrations are provided for all taxa with notes provided on each genus. Excluded and doubtful genera are listed with notes on their taxonomy and phylogeny. Taxonomic keys are provided for all revised families with morphological details for genera within the families.
Diabetic dyslipidaemia C. Thambiah, Subashini; Lai, Leslie Charles
Practical laboratory medicine,
08/2021, Volume:
26
Journal Article
Peer reviewed
Open access
Diabetes mellitus (DM) is an escalating pandemic and an established cardiovascular risk factor. An important aspect of the interaction between DM and atherosclerotic cardiovascular disease (ASCVD) is ...diabetic dyslipidaemia, an atherogenic dyslipidaemia encompassing quantitative hypertriglyceridaemia (hyperTG) and decreased high density lipoprotein cholesterol (HDL) and qualitative increased small dense low density lipoprotein cholesterol (sdLDL) particles, large very low density lipoprotein cholesterol (VLDL) subfraction (VLDL1) and dysfunctional HDL modifications in lipoproteins. Much of the pathophysiology linking DM and dyslipidaemia has been elucidated. This paper aims to review the pathophysiology and management of diabetic dyslipidaemia with respect to ASCVD. Briefly, the influence of diabetic kidney disease on lipid profile and lipid changes causing type 2 diabetes mellitus are highlighted. Biomarkers of diabetic dyslipidaemia, including novel markers and clinical trials that have demonstrated that non-lipid and lipid lowering therapies can lower cardiovascular risk in diabetics are discussed. The stands of various international guidelines on lipid management in DM are emphasised. It is important to understand the underlying mechanisms of diabetic dyslipidaemia in order to develop new therapeutic strategies against dyslipidaemia and diabetes. The various international guidelines on lipid management can be used to tailor a holistic approach specific to each patient with diabetic dyslipidaemia.
Objective This study aimed to determine the prevalence of vertebral fractures (VF) in a selected urban population in Malaysia and to explore possible variables associated with VF in the study ...population. Methods A cross-sectional study involving community-living, healthy subjects aged between 45-90 years from the state of Selangor, Malaysia, were invited to attend a bone health check-up. Subjects with diseases known to affect bone metabolism or were on treatment for osteoporosis (OP) were excluded. Bone mineral density (BMD) was measured using dual energy X-ray absorptiometry (DXA). Lateral and antero-posterior view lumbar spine x-rays were performed and VF was determined by the semi-quantitative Genant method. Results A total of 386 subjects were studied. Asymptomatic morphometric VF were found in 44 (11.4%) subjects. T12 was the most common vertebrae to be fractured. The prevalence of VF was significantly higher in menopausal women (12.4%) compared to non-menopausal women, in those above the age of 60 (18.5%), in those of Chinese ethnicity (16.5%), in those with a low body fat percentage (17.1%) and among those with OP (27.0%). The mean (standard deviation) 25-hydroxyvitamin D 25(OH)D levels were significantly higher in those with VF compared to those without VF, 67.64 (23.50) and 57.47 (21.71) nmol/L, respectively. However, after multiple regression analysis, age over 60 years and OP on DXA BMD measurement were the only significant associated factors for VF. Conclusion Overall, 11.4% of a selected Malaysian urban population had asymptomatic morphometric VF. Age over 60 years and OP on DXA BMD measurement, but not 25(OH)D levels, were associated with VF.
Bone-turnover marker (BTM) measurements in the blood or urine reflect the bone-remodeling rate and may be useful for studying and clinically managing metabolic bone diseases. Substantial evidence ...supporting the diagnostic use of BTMs has accumulated in recent years, together with the publication of several guidelines. Most clinical trials and observational and reference-interval studies have been performed in the Northern Hemisphere and have mainly involved Caucasian populations. This review focuses on the available data for populations from the Asia-Pacific region and offers guidance for using BTMs as diagnostic biomarkers in these populations. The procollagen I N-terminal propeptide and β-isomerized C-terminal telopeptide of type-I collagen (measured in plasma) are reference BTMs used for investigating osteoporosis in clinical settings. Premenopausal reference intervals (established for use with Asia-Pacific populations) and reference change values and treatment targets (used to monitor osteoporosis treatment) help guide the management of osteoporosis. Measuring BTMs that are not affected by renal failure, such as the bone-specific isoenzyme alkaline phosphatase and tartrate-resistant acid phosphatase 5b, may be advantageous for patients with advanced chronic kidney disease. Further studies of the use of BTMs in individuals with metabolic bone disease, coupled with the harmonization of commercial assays to provide equivalent results, will further enhance their clinical applications.
This paper is the seventh in the Fungal Diversity Notes series, where 131 taxa accommodated in 28 families are mainly described from
Rosa
(
Rosaceae
) and a few other hosts. Novel fungal taxa are ...described in the present study, including 17 new genera, 93 new species, four combinations, a sexual record for a species and new host records for 16 species.
Bhatiellae
,
Cycasicola
,
Dactylidina
,
Embarria
,
Hawksworthiana
,
Italica
,
Melanocucurbitaria
,
Melanodiplodia
,
Monoseptella
,
Uzbekistanica
,
Neoconiothyrium
,
Neopaucispora
,
Pararoussoella
,
Paraxylaria
,
Marjia
,
Sporormurispora and Xenomassariosphaeria
are introduced as new ascomycete genera. We also introduce the new species
Absidia jindoensis
,
Alternaria doliconidium
,
A
.
hampshirensis
,
Angustimassarina rosarum
,
Astragalicola vasilyevae
,
Backusella locustae
,
Bartalinia rosicola
,
Bhatiellae rosae
,
Broomella rosae
,
Castanediella camelliae
,
Coelodictyosporium rosarum
,
Comoclathris rosae
,
C
.
rosarum
,
Comoclathris rosigena
,
Coniochaeta baysunika
,
C. rosae
,
Cycasicola goaensis
,
Dactylidina shoemakeri
,
Dematiopleospora donetzica
,
D
.
rosicola
,
D
.
salsolae
,
Diaporthe rosae
,
D
.
rosicola
,
Endoconidioma rosae
-
hissaricae
,
Epicoccum rosae
,
Hawksworthiana clematidicola
,
H
.
lonicerae
,
Italica achilleae
,
Keissleriella phragmiticola
,
K
.
rosacearum
,
K
.
rosae
,
K
.
rosarum
,
Lophiostoma rosae
,
Marjia tianschanica
,
M
.
uzbekistanica
,
Melanocucurbitaria uzbekistanica
,
Melanodiplodia tianschanica
,
Monoseptella rosae
,
Mucor fluvius
,
Muriformistrickeria rosae
,
Murilentithecium rosae
,
Neoascochyta rosicola
,
Neoconiothyrium rosae
,
Neopaucispora rosaecae
,
Neosetophoma rosarum
,
N
.
rosae
,
N
.
rosigena
,
Neostagonospora artemisiae
,
Ophiobolus artemisiicola
,
Paraconiothyrium rosae
,
Paraphaeosphaeria rosae
,
P
.
rosicola
,
Pararoussoella rosarum
,
Parathyridaria rosae
,
Paraxylaria rosacearum
,
Penicillium acidum
,
P
.
aquaticum
,
Phragmocamarosporium rosae
,
Pleospora rosae
,
P
.
rosae
-
caninae
,
Poaceicola agrostina
,
P
.
arundinicola
,
P
.
rosae
,
Populocrescentia ammophilae
,
P
.
rosae
,
Pseudocamarosporium pteleae
,
P
.
ulmi
-
minoris
,
Pseudocercospora rosae
,
Pseudopithomyces rosae
,
Pseudostrickeria rosae
,
Sclerostagonospora lathyri
,
S
.
rosae
,
S
.
rosicola
,
Seimatosporium rosigenum
,
S
.
rosicola
,
Seiridium rosarum
,
Setoseptoria arundelensis
,
S
.
englandensis
,
S
.
lulworthcovensis
,
Sigarispora agrostidis
,
S
.
caryophyllacearum
,
S
.
junci
,
S
.
medicaginicola
,
S
.
rosicola
,
S
.
scrophulariae
,
S
.
thymi
,
Sporormurispora atraphaxidis
,
S
.
pruni
,
Suttonomyces rosae
,
Umbelopsis sinsidoensis
,
Uzbekistanica rosae
-
hissaricae
,
U
.
yakutkhanika
,
Wojnowicia rosicola
,
Xenomassariosphaeria rosae
. New host records are provided for
Amandinea punctata
,
Angustimassarina quercicola
,
Diaporthe rhusicola
,
D. eres
,
D. foeniculina
,
D. rudis
,
Diplodia seriata
,
Dothiorella iberica
,
Lasiodiplodia theobromae
,
Lecidella elaeochroma
,
Muriformistrickeria rubi
,
Neofusicoccum australe
,
Paraphaeosphaeria michotii
,
Pleurophoma pleurospora
,
Sigarispora caulium
and
Teichospora rubriostiolata
. The new combinations are
Dactylidina dactylidis
(=
Allophaeosphaeria dactylidis
),
Embarria clematidis
(=
Allophaeosphaeria clematidis
),
Hawksworthiana alliariae
(=
Dematiopleospora alliariae
) and
Italica luzulae
(=
Dematiopleospora luzulae
). This study also provides some insights into the diversity of fungi on
Rosa
species and especially those on
Rosa
spines that resulted in the characterisation of eight new genera, 45 new species, and nine new host records. We also collected taxa from
Rosa
stems and there was 31% (20/65) overlap with taxa found on stems with that on spines. Because of the limited and non-targeted sampling for comparison with collections from spines and stems of the same host and location, it is not possible to say that the fungi on spines of
Rosa
differ from those on stems. The study however, does illustrate how spines are interesting substrates with high fungal biodiversity. This may be because of their hard structure resulting in slow decay and hence are suitable substrates leading to fungal colonisation. All data presented herein are based on morphological examination of specimens, coupled with phylogenetic sequence data to better integrate taxa into appropriate taxonomic ranks and infer their evolutionary relationships.
The genera Lophiostoma, Misturatosphaeria and several other allied taxa in Lophiostomataceae are revisited. Accounts of these taxa, including their history, morphology, and family placement, based on ...molecular phylogeny, are provided. Type or representative specimens of Lophiostoma and Misturatosphaeria were examined and fresh specimens were obtained from Germany, Italy, Japan and Thailand. A multi-gene phylogenetic analysis of the lophiostomataceous genera Floricola, Lophiostoma, Misturatosphaeria and related taxa is provided. Sixteen genera including Lophiostoma, Lophiohelichrysum, Dimorphiopsis, Platystomum and Vaginatispora, plus eleven newly introduced genera Biappendiculispora, Alpestrisphaeria, Capulatispora, Coelodictyosporium, Guttulispora, Lophiopoacea, Neotrematosphaeria, Paucispora, Pseudolophiostoma, Pseudoplatystomum and Sigarispora are accepted in Lophiostomataceae based on morphology and phylogeny. Lophiostoma caulium, Lophiostoma arundinis and Lophiostoma caudatum are accommodated in Sigarispora. Lophiostoma winteri and Lophiostoma fuckelii are placed in the genera Lophiopoacea and Vaginatispora respectively. Three Curreya species and Misturatosphaeria claviformis are transferred to a new genus, Neocurreya. All other Misturatosphaeria species except Misturatosphaeria aurantiinotata and M. uniseptata are separated in the new genera Asymmetrispora, Aurantiascoma, Magnibotryascoma, Pseudoaurantiascoma and Pseudomisturatosphaeria based on their morphological and phylogenetic affinities. Another new genus, Ramusculicola is introduced for a new collection from Thailand. These seven new genera are accommodated in a new family Floricolaceae, together with Floricola and Misturatosphaeria. Several massarina-like species clustered as a sister clade to Amorosia littoralis and are accommodated in a new genus Angustimassarina. A new family Amorosiaceae is proposed to accommodate the genera Amorosia and Angustimassarina. The putatively named species Decaisnella formosa and Thyridaria macrostomoides form a separate clade together with a new genus Lignosphaeria which is placed in Dothideomycetes, genera incertae sedis.
Acquired methemoglobinemia, predominantly due to oxidizing medications occurs when heme iron in hemoglobin is oxidized from ferrous to ferric ion and binds oxygen irreversibly leading to functional ...anemia, cyanosis, and tissue hypoxia. We report a case of a 60-year-old man with multiple comorbidities who was diagnosed with coronavirus disease 2019 (COVID-19) and developed methemoglobinemia after consumption of prescribed supplements. He presented with dyspnea and cyanosis. An oxygen saturation gap with characteristic chocolate-brown arterial blood indicated methemoglobinemia. Outsourced methemoglobin (MetHb) was increased at 9.0%. Despite aggressive intervention, he succumbed to his illness. In this case, we discuss the pathophysiology of why some individuals, especially the elderly with COVID-19 are more susceptible to develop methemoglobinemia after possibly being exposed to oxidizing agents. Laboratory methods for assessing oxygen saturation, including pulse oximetry, arterial blood gas and co-oximetry are examined in relation to this case. The importance of considering a diagnosis of methemoglobinemia based on clinical and biochemical findings although MetHb assay or co-oximetry are not readily available is also emphasized.
•Acute methemoglobinemia occurs when heme iron is oxidized from ferrous to ferric ion in Hb.•Ferric iron is unable to bind and transport oxygen leading to functional anaemia, cyanosis, and tissue hypoxia.•Causes of methemoglobinemia can be congenital or more commonly, acquired due to exposure to oxidizing substances.•Refractory hypoxia, chocolate-brown arterial blood, and oxygen saturation gap are diagnostic clues.•Disease severity in elderly COVID-19 patients is further exacerbated by the pro-inflammatory properties of MetHb.
Osteoporosis (OP) is a condition where there is low bone density and microarchitectural deterioration which can predispose to fragility fractures. There is a wealth of literature on OP from the ...developed countries, but less so from Asia. This review will explore the field of OP research in South-East Asia with regard to the epidemiology, the diagnosis of OP and the role of laboratory tests in the management of OP, with emphasis on 25-dihydroxyvitamin D and bone turnover markers.
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