A light water nuclear Reactor has been exergy analyzed, and the rate of irreversible exergy loss and exergy destruction is calculated for each of its components. The ratio of these losses compared to ...the total input exergy loss is calculated, which shows that most irreversible losses occur in the reactors, turbines, steam generators, respectively, as well as in the downstream operations. The main aim of this paper is to optimize the power plant using an innovative firefly algorithm and then to propose a novel strategy to improve the overall performance of the plant. As shown in the results, the exergy destruction rate of the plant decreased by 1.18% using the firefly method, and the exergy efficiency of the plant reached 29.3% comparing to the operational amount of 28.99%. Also, the results of the firefly optimization process compared to the Genetic algorithm and gravitational search algorithm to study the accuracy of the model for exergy analysis fitness problems in the power plants and the results of this comparison has shown that the results are nearly similar in the mentioned methods. However, the firefly is faster and more accurate in limited iterations.
•Iranian Bushehr reactor can be developed to a 1000 MW electricity generation power plant.•The firefly optimization method is the most reliable technic for thermodynamic optimization.•Exergy analysis is more accurate for performance analysis in the nuclear power plants.•A fossil-nuclear hybrid plant show better performance compared to the stand-alone nuclear plants.
The tendency to renewables is one of the consequences of changing attitudes towards energy issues. As a result, solar energy, which is the leader among renewable energies based on availability and ...potential, plays a crucial role in full filing global needs. Significant problems with the solar thermal power plants (STPP) are the operation time, which is limited by daylight and is approximately half of the power plants with fossil fuels, and the capital cost. Exergy analysis survey of STPP hybrid with PCM storage carried out using Engineering Equation Solver (EES) program with genetic algorithm (GA) for three different scenarios, based on eight decision variables, which led us to decrease final product cost (electricity) in optimized scenario up to 30% compare to base case scenario from 28.99 $/kWh to 20.27 $/kWh for the case study. Also, in the optimal third scenario of this plant, the inner carbon dioxide gas cycle produces 1200 kW power with a thermal efficiency of 59% and also 1000 m3/h water with an exergy efficiency of 23.4% and 79.70 kg/h with an overall exergy efficiency of 34% is produced in the tetrageneration plant.
•This paper presents a new cogeneration plant named nuclear tetrageneration.•In the tetrageneration plants a combined water, heat, power and chemicals generation occurs.•SMR reactor is used in this plant as a source of power supported with the solar thermal collectors.•Tetrageneration plant provides a 45% increase in the overall exergy efficiency of the plant.•Formic acid which was produced in this plant by solar fuel technology can be used as a chemical energy storage.
This paper proposes using sodium-cooled fast reactor technologies for use in hydrogen vapor methane (SMR) modification. Using three independent energy rings in the Russian BN-600 fast reactor, steam ...is generated in one of the steam-generating cycles with a pressure of 13.1 MPa and a temperature of 505 °C. The reactor's second energy cycles can increase the gas-steam mixture's temperature to the required amount for efficient correction. The 620 ton/hr 540 °C steam generated in this cycle is sufficient to supply a high-temperature synthesis current source (700 °C), which raises the steam-gas mixture's temperature in the reactor. The proposed technology provides a high rate of hydrogen production (approximately 144.5 ton/hr of standard H2), also up to 25% of the original natural gas, in line with existing SMR technology for preparing and heating steam and gas mixtures will be saved. Also, exergy analysis results show that the plant's efficiency reaches 78.5% using HTR heat for combined hydrogen and power generation.
•A microarray meta-analysis revealed 32 dysregulated circRNAs in gastric adenocarcinoma.•hsa_circ_0002019 & hsa_circ_0074736 showed no significant fold change in plasma.•hsa_circ_0002019 & ...hsa_circ_0074736 regulate 4 mRNAs affecting gastric cancer survival.
Gastric cancer is the fourth leading cause of cancer-related mortality and one of the most commonly diagnosed malignancies worldwide. Gastric adenocarcinoma (GAC) accounts for the majority of gastric cancer cases. Circular RNAs (circRNAs) have been shown to be associated with carcinogenesis and cancer progression. This research aims to investigate GAC-associated circRNAs and the underlying mechanisms of circRNA-miRNA-mRNA networks in the development and progression of GAC. Differentially expressed miRNAs and mRNAs (DEMs and DEGs) were identified in Gene Expression Omnibus (GEO) microarray datasets using the R package Limma. A microarray meta-analysis was performed to identify potential GAC-associated circRNAs with high statistical power, resulting in 13 up-regulated and 19 down-regulated circRNAs. CircRNA-miRNA-mRNA networks were constructed by combining predicted and experimentally validated databases and considering differentially expressed miRNAs and mRNAs. The constructed ceRNA networks revealed the potential regulatory effect of hsa_circ_0002019 and hsa_circ_0074736 on key survival-related genes. The expression levels of these two circRNAs were measured in plasma samples from GAC patients and healthy controls using SYBR Green-based real-time PCR. Axon guidance, cellular senescence, AGE-RAGE signaling pathway in diabetic complications, and AMPK signaling pathway were among the major significant (P-value <0.05) enriched pathways of "main mRNAs" in the constructed ceRNA networks. In conclusion, we identified strongly correlated circRNAs and their likely mechanisms of action in GAC, which may improve the knowledge of regulatory networks underlying GAC formation and contribute to developing better strategies for early diagnosis, prognosis, and treatment.
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Chronic myeloid leukemia (CML) is a model of leukemogenesis in which the exact molecular mechanisms underlying blast crisis still remained unexplored. The current study identified multiple common and ...rare important findings in myeloid blast crisis CML (MBC-CML) using integrated genomic sequencing, covering all classes of genes implicated in the leukemogenesis model. Integrated genomic sequencing via Whole Exome Sequencing (WES), Chromosome-seq and RNA-sequencing were conducted on the peripheral blood samples of three CML patients in the myeloid blast crisis. An in-house filtering pipeline was applied to assess important variants in cancer-related genes. Standard variant interpretation guidelines were used for the interpretation of potentially important findings (PIFs) and potentially actionable findings (PAFs). Single nucleotide variation (SNV) and small InDel analysis by WES detected sixteen PIFs affecting all five known classes of leukemogenic genes in myeloid malignancies including signaling pathway components (ABL1, PIK3CB, PTPN11), transcription factors (GATA2, PHF6, IKZF1, WT1), epigenetic regulators (ASXL1), tumor suppressor and DNA repair genes (BRCA2, ATM, CHEK2) and components of spliceosome (PRPF8). These variants affect genes involved in leukemia stem cell proliferation, self-renewal, and differentiation. Both patients No.1 and No.2 had actionable known missense variants on ABL1 (p.Y272H, p.F359V) and frameshift variants on ASXL1 (p.A627Gfs*8, p.G646Wfs*12). The GATA2-L359S in patient No.1, PTPN11-G503V and IKZF1-R208Q variants in the patient No.3 were also PAFs. RNA-sequencing was used to confirm all of the identified variants. In the patient No. 3, chromosome sequencing revealed multiple pathogenic deletions in the short and long arms of chromosome 7, affecting at least three critical leukemogenic genes (IKZF1, EZH2, and CUX1). The large deletion discovered on the short arm of chromosome 17 in patient No. 2 resulted in the deletion of TP53 gene as well. Integrated genomic sequencing combined with RNA-sequencing can successfully discover and confirm a wide range of variants, from SNVs to CNVs. This strategy may be an effective method for identifying actionable findings and understanding the pathophysiological mechanisms underlying MBC-CML, as well as providing further insights into the genetic basis of MBC-CML and its management in the future.
Introduction
Nephrogenic diabetes insipidus (NDI) is a rare genetic disease that causes water imbalance. The kidneys play a crucial role in regulating body fluids by controlling water balance through ...urine excretion. This highlights their essential function in managing the body's water levels, but individuals with NDI may have excess urine production (polyuria), that leads to excessive thirst (polydipsia). Untreated affected individuals may exhibit poor feeding and failure to thrive. This disease is caused by mutations in the AVPR2 and the AQP2 genes which have the X‐linked and autosomal recessive/dominant inheritance, respectively. Both of these genes are expressed in the kidney.
Methods
Twelve Iranian patients from 10 consanguineous families were studied in this project. DNA was extracted from the whole blood samples of the patients and their parents. All coding exons and exon‐intron boundaries of the AVPR2 and AQP2 genes were sequenced in the affected individuals, and the identified variants were investigated in the parents. All variants were analyzed according to the ACMG (American College of Medical Genetics and Genomics) guidelines.
Results
In this study, 6 different mutations were identified in the patients, including 5 in the AQP2 gene (c.439G>A, c.538G>A, c.140C>T, c.450T>A, and the novel c.668T>C) and 1 in the AVPR2 gene (c.337C>T) in the present study.
Discussion
As expected, all the detected mutations in this study were missense. According to the ACMG guideline, the identified mutations were categorized as pathogenic or likely pathogenic. Unlike previous studies which showed more than 90% of mutations were in the AVPR2 gene, and only less than 10% of the mutations were in the AQP2 gene, it was found that more than 90% of our identified mutations located in the AQP2 gene, and only one mutation was observed in the AVPR2 gene, which seems it may be a result of the high rate of consanguineous marriages in the Iranian population. We observed genotype‐phenotype correlation in some of our affected individuals, and some of the mutations were observed in unrelated families from same ethnicity which could be suggestive of a founder mutation.
The new identified mutation found in the present study.
CDC27 is one of the core components of Anaphase Promoting complex/cyclosome. The main role of this protein is defined at cellular division to control cell cycle transitions. Here we review the ...molecular aspects that may affect CDC27 regulation from cell cycle and mitosis to cancer pathogenesis and prognosis.
It has been suggested that CDC27 may play either like a tumor suppressor gene or oncogene in different neoplasms. Divergent variations in CDC27 DNA sequence and alterations in transcription of CDC27 have been detected in different solid tumors and hematological malignancies. Elevated CDC27 expression level may increase cell proliferation, invasiveness and metastasis in some malignancies. It has been proposed that CDC27 upregulation may increase stemness in cancer stem cells. On the other hand, downregulation of CDC27 may increase the cancer cell survival, decrease radiosensitivity and increase chemoresistancy. In addition, CDC27 downregulation may stimulate efferocytosis and improve tumor microenvironment.
CDC27 dysregulation, either increased or decreased activity, may aggravate neoplasms. CDC27 may be suggested as a prognostic biomarker in different malignancies.
X-linked congenital adrenal hypoplasia due to NR0B1 mutation is characterized by hypogonadotropic hypogonadism (HH) and infertility. Here, we describe a novel pathogenic frameshift variant in NR0B1 ...associated with congenital adrenal hypoplasia by whole exome sequencing in an Iranian case with high level of testosterone. Clinical evaluations and pedigree drawing were performed. Point mutations, gene conversions, and large deletions of the CYP21A2 gene were checked. WES and segregation analyses were conducted. In silico analysis was also performed for the novel variant. The ACTH, 17-hydroxy progesterone c, and DHEA sulfate values were elevated up to 624.6 pg/mL, 8.6 pmol/L, and 17.8UMOL/L, respectively. No mutation was found in the CYP21A2 gene. WES identified a novel hemizygous frameshift insertion c.218_219insACCA: p.His73GlnfsTer41 variant in the NR0B1 gene with a pathogenic effect according to ACMG criteria. Genetic testing is helpful for differential diagnosis in primary adrenal insufficiency disorders. NR0B1 may be a common cause of congenital adrenal hypoplasia in our population.
CD4
+
T cells are known as a noteworthy potential modulator of inflammation in multiple sclerosis (MS). In the current study, we investigated the transcriptome profile of CD4
+
T cells in patients ...with relapsing–remitting MS (RRMS) at the relapse phase. We performed RNA sequencing of CD4
+
T cells isolated from four relapsing–remitting MS (RRMS) patients at the relapse phase and four age- and sex-matched healthy controls. The edgeR statistical method was employed to determine differentially expressed genes (DEGs). Gene set enrichment analysis was subsequently performed. Applying a physical interaction network, genes with higher degrees were selected as hub genes. A total of 1278 and 1034 genes were defined at significantly higher or lower levels, respectively, in CD4
+
T cells of RRMS patients at the relapse phase as compared with healthy controls. The top up- and downregulated genes were
JAML
and
KDM3A.
The detected DEGs were remarkable on chromosomes 1 and 2, respectively. The DEGs were mainly enriched in the pathways “regulation of transcription, DNA-templated,” “regulation of B cell receptor signaling pathway,” “protein phosphorylation,” “epidermal growth factor receptor signaling pathway,” and “positive regulation of neurogenesis.” Moreover, 16 KEGG pathways mostly associated with the immune system and viral infections were enriched. In the constructed physical interaction networks,
UBA52
and
TP53
were shown to be the most highly ranked hub genes among upregulated and downregulated genes, respectively. By applying global transcriptome profiling of CD4
+
T cells, we deciphered the involvement of several novel genes and pathways in MS pathogenesis. The present results must be confirmed by in vivo and in vitro studies.
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