t(6;9)(p22;q34.1)/DEK-NUP214 is a recurrent genetic abnormality that occurs in 1–2% of patients with acute myeloid leukemia (AML), and rarely in myelodysplastic syndrome (MDS). It has been suggested ...by others that all myeloid neoplasms with t(6;9)/DEK-NUP214 may be considered as AML, even when blast count is <20%. In this study, we compared the clinicopathologic features of 107 patients with myeloid neoplasms harboring t(6;9)/DEK-NUP214: 33 MDS and 74 AML. Compared with patients with AML, patients with MDS were older (p = 0.10), had a lower white blood cell count (p = 0.0017), a lower blast count in the peripheral blood (p < 0.0001) and bone marrow (p < 0.0001), a higher platelet count (p = 0.022), and a lower frequency of FLT3-ITD mutation (p = 0.01). In addition, basophilia was not a common feature in the patients of this cohort. Although there was no difference in overall survival between MDS and AML patients (p = 0.18) in the entire cohort, the survival curves did show a trend toward favorable survival in MDS patients. Multivariate analyses showed that initial diagnosis of MDS vs. AML and allogeneic hematopoietic stem cell transplantation were prognostic factors for survival of patients with t(6;9)/DEK-NUP214 (p = 0.008 and p < 0.0001, respectively). Our data suggest that MDS with t(6;9)/DEK-NUP214 is prognostically not equivalent to AML with t(6;9)/DEK-NUP214. These data also show that stem cell transplantation greatly improves the survival of MDS and AML patients with myeloid neoplasms associated with t(6;9)/DEK-NUP214.
The survival and sustainability of regional species is constrained by habitat quality. In recent decades, the intensification of human activities on a global scale has had a profound impact on ...regional ecosystems and poses a serious threat to regional sustainable development. Scientific measurement of the drivers of habitat quality can provide important support for the development of effective biodiversity conservation and sustainable land-use policies. Taking the Hung River Valley as an example, the InVEST model was used to assess the habitat quality of the study area in 2000, 2005, 2010, 2015, and 2020 and to explore its spatial and temporal variation and distribution characteristics in combination with the spatial autocorrelation model, and the geographically weighted regression (GWR) model was used to explore the drivers of habitat quality change. The results show the following: (1) The overall habitat quality shows an increasing trend during 2000–2020, but the expansion of construction land in the central region plays a dominant role in the degradation of regional habitat quality. (2) The “Guide-Ledu” line is the dividing line of habitat quality in the Hung River Valley, with a general distribution of “south is good, north is bad” and “south is hot, north is cold”. (3) Natural factors such as slope and elevation basically shape the overall distribution pattern of habitat quality, while urbanisation factors such as population density, gross domestic product, and the night-time lighting index are generally negatively correlated with habitat quality. The results of the study can reveal the linkage between ecosystems and land-use change in the context of urbanisation.
Fusion partners of
KMT2A
affect disease phenotype and influence the current World Health Organization classification of hematologic neoplasms. The t(11;16)(q23;p13)/
KMT2A-CREBBP
is considered ...presumptive evidence of a myelodysplastic syndrome (MDS) and a MDS-related cytogenetic abnormality in the classification of acute myeloid leukemia (AML). Here, we report 18 cases of hematologic neoplasms with t(11;16). There were 8 males and 10 females with a median age of 51.9 years at time of detection of t(11;16). Of 17 patients with enough clinical information and pathological materials for review, 16 had a history of cytotoxic therapies for various malignancies including 12/15 patients who received topoisomerase II inhibitors, and 15 were classified as having therapy-related neoplasms. The median interval from the diagnosis of primary malignancy to the detection of t(11;16) was 23.2 months. Dysplasia, usually mild, was observed in 7/17 patients. Blasts demonstrated monocytic differentiation in 8/8 patients who developed AML at the time or following detection of t(11;16). t(11;16) was observed as the sole chromosomal abnormality in 10/18 patients.
KMT2A
rearrangement was confirmed in 11/11 patients. The median survival from the detection of t(11;16) was 15.4 months. In summary, t(11;16)(q23;p13) is rare and overwhelmingly associated with prior exposure of cytotoxic therapy. Instead of being considered presumptive evidence of myelodysplasia, we suggest that the detection of t(11;16) should automatically prompt a search for a history of malignancy and cytotoxic therapy so that proper risk stratification and clinical management are made accordingly. The dismal outcome of patients with t(11;16) is in keeping with that of therapy-related neoplasms.
Urban agglomerations have gradually formed in different Chinese cities, exerting great pressure on the ecological environment. Ecosystem health is an important index for the evaluation of the ...sustainable development of cities, but it has rarely been used for urban agglomerations. In this study, the ecosystem health in the middle reaches of the Yangtze River Urban Agglomeration was assessed using the ecosystem vigor, organization, resilience, and services framework at the county scale. A GeoDetector was used to determine the effects of seven factors on ecosystem health. The results show that: (1) The spatial distribution of ecosystem health differs significantly. The ecosystem health in the centers of Wuhan Metropolis, Changsha-Zhuzhou-Xiangtan City Group, and Poyang Lake City Group is significantly lower than in surrounding areas. (2) Temporally, well-level research units improve gradually; research units with relatively weak levels remain relatively stable. (3) The land use degree is the main factor affecting ecosystem health, with interactions between the different factors. The effects of these factors on ecosystem health are enhanced or nonlinear; (4) The effect of the proportion of construction land on ecosystem health increases over time. The layout used in urban land use planning significantly affects ecosystem health.
A new diketopiperazine (DKP) derivative, (6 R ,3 Z)-3-benzylidene-6-isobutyl-1-methyl piperazine-2,5-dione (1), as well as five known DKPs 2 – 6 was isolated from a deep sea-derived Streptomyces sp. ...SCSIO 04496. The structure of 1 was elucidated using a combination of 1D and 2D NMR, HR-ESI-MS and chiral-phase HPLC techniques. Compounds 1 – 6 did not show cytotoxic activity at a concentration of 100 μM in bioactivity assay.
As a transitional zone between urban and rural areas, the peri-urban areas are the areas with the most intense urban expansion and the most frequent spatial reconfiguration, and in this context, it ...is particularly important to reveal the evolution pattern of rural settlements in the peri-urban areas to provide reference for the rearrangement of rural settlements. The study takes five townships in the urban suburbs, and explores the scale, shape, spatial layout, and spatial characteristics of the urban suburbs of Hefei from 1980 to 2030 under the influence of urban-lake symbiosis based on spatial mathematical analysis and geographical simulation software. The study shows that: (1) the overall layout of rural settlements in the study area is randomly distributed due to the hilly terrain, but in small areas there is a high and low clustering phenomenon, and the spatial density shows the distribution characteristics of "high in the east and low in the west"; (2) since the reform and opening up, there are large spatial differences in the scale of rural settlements in the study area. (3) Different development scenarios have a strong impact on the future spatial pattern of rural settlement land use within the study area, which is a strong reflection of policy.
Lymphoblastic leukemia (ALL) following myelodysplastic syndrome (MDS) or myelodysplastic/myeloproliferative neoplasm (MDS/MPN) is very rare. We report five cases: four had ALL diagnosed after MDS or ...MDS/MPN and one had ALL and MDS diagnosed simultaneously. At the onset of ALL, all patients showed co-existing MDS or MDS/MPN. Map-back FISH was performed in four patients, showing that ALL and MDS were cytogenetically related in two patients and unrelated in the other two patients. All five patients were treated with ALL-based chemotherapies, two patients with ALL clonally related to MDS were refractory to the therapies, whereas the other three patients achieved remission. We conclude that ALL developed after MDS is extremely rare. In some patients, ALL is clonally related to MDS and these patients may be refractory to ALL-based chemotherapies. In other patients who have no evidence of clonal relation between ALL and MDS, these patients more likely respond to ALL-based treatment regimens.
We design and implement the first private and anonymous decentralized crowdsourcing system ZebraLancer, and overcome two fundamental challenges of decentralizing crowdsourcing, i.e. data leakage and ...identity breach. First, our outsource-then-prove methodology resolves the tension between blockchain transparency and data confidentiality, which is critical in crowdsourcing use-case. ZebraLancer ensures: (i) a requester will not pay more than what data deserve, according to a policy announced when her task is published via the blockchain; (ii) each worker indeed gets a payment based on the policy, if he submits data to the blockchain; (iii) the above properties are realized not only without a central arbiter, but also without leaking the data to the open blockchain. Furthermore, the transparency of blockchain allows one to infer private information about workers and requesters through their participation history. On the other hand, allowing anonymity will enable a malicious worker to submit multiple times to reap rewards. ZebraLancer overcomes this problem by allowing anonymous requests/submissions without sacrificing the accountability. The idea behind is a subtle linkability: if a worker submits twice to a task, anyone can link the submissions, or else he stays anonymous and unlinkable across tasks. To realize this delicate linkability, we put forward a novel cryptographic concept, i.e. the common-prefix-linkable anonymous authentication. We remark the new anonymous authentication scheme might be of independent interest. Finally, we implement our protocol for a common image annotation task and deploy it in a test net of Ethereum. The experiment results show the applicability of our protocol with the existing real-world blockchain.
Antibiotics are widely used in the treatment of bacterial infections and as food additives in the livestock industry. The wide usage of antibiotics causes residues in animal products, like milk, eggs ...and meat. A number of studies have reported that antibiotic residues exist at high concentrations in watercourses around the world. Doxycycline (DH), oxytetracycline (OTCC) and florfenicol (FF) are the three most commonly used veterinary antibiotics in China. However, studies of the toxic effects of DH, OTCC and FF are limited. In this study, six-moth-old healthy male adult zebrafish were exposed to 0, 10, 30, 100 μg/L DH, OTCC or FF for 21 days. After exposure, some biochemical parameters changed significantly, including total cholesterol (TC), triglyceride (TG), pyruvate and acid phosphatase (ACP). In addition, mucus secretion in the gut decreased and the transcription of related genes also decreased significantly. Moreover, the composition of microbiota in the gut changed significantly. DH, OTCC and FF exposure caused the decrease of diversity of gut microbiota. The relative abundance of Proteobacteria increased significantly after OTCC and FF exposure and Fusobacteria decreased in all antibiotic-treated groups. Further functional prediction analysis also suggested changes in gut microbiota in the OTCC and FF-treated groups, especially those linked to metabolism. To support this idea, we confirmed that some glycolipid related genes also increased significantly in the liver of adult zebrafish after antibiotic exposure. According to these results, DH, OTCC or FF exposure could cause the gut microbiota dysbiosis and dysfunction, and hepatic metabolic disorder in adult male zebrafish.
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•Doxycycline, oxytetracycline and florfenicol decreased gut mucus secretion.•Antibiotic exposure induced gut microbiota dysbiosis and dysfunction.•The hepatic glycolipid metabolism was also affected.
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