The objective of this study is to characterize Streptococcus pyogenes isolates with a mucoid phenotype and to compare them with non-mucoid isolates obtained between April and August 2016.
...Identification and antimicrobial susceptibility were performed in all isolates. The emm type and exotoxin genes speA, speB, speC, speF, speG, speH, speJ, speZ and ssa were analyzed. Clinical and demographic data were collected.
From 96 isolates analyzed, 47% had a mucoid phenotype and 95.5% of them presented speA-speB-speF-speG-ssa genes and emm3 genotype. The main clinical manifestation was pharyngotonsillitis (77.1%) evolving to scarlet fever in 67.5% of the cases.
This study describes the circulation of a mucoid phenotype strain with a speA-speB-speF-speG-ssa toxin profile and emm3.1 genotype considered one of the most frequent and virulent of SGA.
El objetivo de este estudio es la caracterización de cepas de Streptococcus pyogenes con fenotipo mucoide y su comparación con las cepas no mucoides aisladas entre abril y agosto de 2016.
Se llevó a cabo la caracterización y el estudio de sensibilidad antimicrobiana de todos los aislados. Se determinó el tipo emm y se analizaron los genes de exotoxinas speA, speB, speC, speF, speG, speH, speJ, speZ y ssa. Se recogieron datos clínicos y demográficos.
De 96 aislados analizados, el 47% presentaron un fenotipo mucoide, y de estos últimos, el 95,5% presentaron los genes speA-speB-speF-speG-ssa y genotipo emm3. La principal manifestación clínica entre todos los pacientes fue faringoamigdalitis (77,1%) que evolucionó a escarlatina en el 67,5% de los casos.
Se describe la circulación de una cepa de aspecto mucoide con perfil de toxinas speA-speB-speF-speG-ssa y genotipo emm3.1 considerado de los más frecuentes y más virulentos de SGA.
Organic olive farming in Andalusia, Spain. A review Pleguezuelo, Carmen Rocío Rodríguez; Zuazo, Víctor Hugo Durán; Martínez, José Ramón Francia ...
Agronomy for sustainable development,
04/2018, Volume:
38, Issue:
2
Journal Article
Peer reviewed
Open access
Olive is a key crop in the Mediterranean basin, socially, economically, and environmentally, being a major source of rural employment. Organic products such as olive oil have attracted many types of ...consumers in recent years. In this context, the Mediterranean basin represents 5 Mha of the 10 Mha of olive farming worldwide, with Spain being the leading producer. Also, during the recent decades, olive farmers met the challenges of sustainability by implementing principles of agroecological production. Therefore, the focal point is no longer exclusively on yields but also on the quality, health, and environmental aspects of products and systems. In this work, we review and analyse the global situation of organic farming, focusing on the development, current status, perspectives, and opportunities of organic olive farming in Andalusia (S Spain). In addition, we assess the environmental benefits and consequences of the shift from conventional to organic management of olive orchards, focusing on the improvement of soil quality and biodiversity. Also, the general factors affecting the decision making for acquiring organic olive oil are discussed. Our main findings show the following: (1) It is necessary to encourage educational and research programmes to promote the demand for these products, positively affecting consumer health, protecting the environment, and improving rural economies. (2) The future of organic agriculture will depend on its economic viability and on the determination shown by governments to protect these practices. (3) Further support for funding research is needed to continue studying the effect of olive cultivation on soil biological quality, changes in soil properties, and biodiversity. More importantly, these beneficial extra outputs produced for society must be interiorised in economic value to compensate organic oil producers. (4) Finally, a clear understanding of attitudes and preferences, as well as the motivations of consumer when making the choice for organic olive products, is essential in responding to this specific demand. However, as stated above, major institutional and educational actions are also needed in order to boost sustainable organic olive farming and thus the preference for organic olive oil.
Swallowed topical-acting corticosteroids are recommended as first-line therapy for eosinophilic esophagitis (EoE). Asthma medications not optimized for esophageal delivery are sometimes effective, ...although given off-label. We performed a randomized, placebo-controlled trial to assess the effectiveness and tolerability of a budesonide orodispersible tablet (BOT), which allows the drug to be delivered to the esophagus in adults with active EoE.
We performed a double-blind, parallel study of 88 adults with active EoE in Europe. Patients were randomly assigned to groups that received BOT (1 mg twice daily; n = 59) or placebo (n = 29) for 6 weeks. The primary end point was complete remission, based on clinical and histologic factors, including dysphagia and odynophagia severity ≤2 on a scale of 0–10 on each of the 7 days before the end of the double-blind phase and a peak eosinophil count <5 eosinophils/high power field. Patients who did not achieve complete remission at the end of the 6-week double-blind phase were offered 6 weeks of open-label treatment with BOT (1 mg twice daily).
At 6 weeks, 58% of patients given BOT were in complete remission compared with no patients given placebo (P < .0001). The secondary end point of histologic remission was achieved by 93% of patients given BOT vs no patients given placebo (P < .0001). After 12 weeks, 85% of patients had achieved remission. Six-week and 12-week BOT administration were safe and well tolerated; 5% of patients who received BOT developed symptomatic, mild candida, which was easily treated with an oral antifungal agent.
In a randomized trial of adults with active EoE, we found that budesonide oral tablets were significantly more effective than placebo in inducing clinical and histologic remission. Eudra-CT number 2014-001485-99; ClinicalTrials.gov ID NCT02434029.
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The neuromuscular junction (NMJ) is the peripheral synapse that controls the coordinated movement of many organisms. The NMJ is also an archetypical model to study synaptic morphology and function. ...As the NMJ is the primary target of neuromuscular diseases and traumatic injuries, the establishment of suitable models to study the contribution of specific postsynaptic muscle-derived proteins on NMJ maintenance and regeneration is a permanent need. Considering the unique experimental advantages of the levator auris longus (LAL) muscle, here we present a method allowing for efficient electroporation-mediated gene transfer and subsequent detailed studies of the morphology and function of the NMJ and muscle fibers. In addition, we have standardized efficient facial nerve injury protocols to analyze LAL muscle NMJ degeneration and regeneration. Our results show that the expression of a control fluorescent protein does not alter either the muscle structural organization, the apposition of the pre- and post-synaptic domains, or the functional neurotransmission parameters of the LAL muscle NMJs; in turn, the overexpression of MuSK, a major regulator of postsynaptic assembly, induces the formation of ectopic acetylcholine receptor clusters. Our NMJ denervation experiments showed complete reinnervation of LAL muscle NMJs four weeks after facial nerve injury. Together, these experimental strategies in the LAL muscle constitute effective methods to combine protein expression with accurate analyses at the levels of structure, function and regeneration of the NMJ.
Using beneficial microorganisms along with sustainable strategies such as agronomic biofortification offers eco-friendly alternatives to combat climate change in ecosystems like dehesas. This study ...analyzes the combined effects of four wild Trichoderma spp. isolated from Extremadura, Spain (T. koningiopsis, two T. gamsii, and T. koningii, with negative and positive controls) and four Zn biofortification treatments (no Zn application; soil application of 5 mg of ZnSOsub.4·7Hsub.2O per kg of soil, labeled soil Zn; two foliar applications of 5 mL 0.5% ZnSOsub.4·7Hsub.2O, labeled foliar Zn; and soil + foliar combination, labeled SF) on Trifolium subterraneum performance. The combination of T. koningiopsis and T. gamsii with foliar Zn improved plant growth by up to 34.4%. Zinc accumulation was about 30% higher when T. gamsii and T. koningii were applied with SF, and their inoculation resulted in a 2.5-fold increase in ash. Trichoderma spp. affected nodulation differently; both T. gamsii inhibited nodulation by 24%, whereas neither T. koningiopsis nor T. koningii showed differences from the controls. These results highlight the potential of combining beneficial microorganisms with biofortification strategies to address nutrient deficiencies and improve agricultural sustainability. However, the complex interactions between both factors underscore the importance of strain selection and call for further research to optimize application strategies and elucidate underlying mechanisms.
The main purposes of this study were to describe teacher education students’ values and degree of satisfaction with life, to analyze whether any differences by educational program, gender or living ...standard and to analyze the association between values and satisfaction with life. A total of 565 students of teacher degree programs (girls 415 (73.5%)) answered a self-administered questionnaire composed by two validates scales about their values (Portrait Values Questionnaire (PVQ) and satisfaction with life (Satisfaction with Life Scale)). The results showed that the most important values were self-direction, benevolence and hedonism, while the least important values were power, tradition and achievements. Females reported higher importance for benevolence, universalism, self-direction, stimulation, hedonism and security. Males reported higher importance for power. Students who live with family reported also more high values for power. Concerning satisfaction with life, it was associated to higher values of power. Current intervention programs have focused different approaches by gender and living standard. Programs focus on increasing satisfaction with life should consider the values structure of students.
Our objective was to improve the results of extracorporeal shock waves lithotripsy using hydroxycitric acid (HCA) like adjuvant therapy. Double blind randomized clinical trial using hydroxycitric ...acid versus placebo (ID NCT05525130). Multicenter study of adjuvant exposure to a food supplement with hydroxycitric acid (HCA), vs. placebo in patients with calcium oxalate and calcium phosphate lithiasis with indication for extracorporeal shock wave lithotripsy (ESWL). 81 patients were included in the study to compare the effect of HCA versus placebo. Stone fragmentation, the main efficacy variable. Other variables analyzed were stone size, Hounsfield Unit Stone and tolerability. Statistical study with SPSS, statistical significance p ≤ 0.05. Eighty-one patients were included, 40 in the intervention group with HCA and 41 in the control group with placebo. The average stone area was 174,36 mm
2
(SD: 32,83 mm
2
) and the average hardness was 1128,11 (SD: 257,65), with no statistically significant differences between groups. Significant statistical differences were obtained in the analysis of the population by intention to treat and by protocol of the main variable, no fragmentation vs. fragmentation where 100% of the patients, who were given ESWL and took HCA, presented fragmentation while 17% of the patients with placebo did not reach fragmentation (p = 0.03). The adjuvant use of HCA in patients for whom ESWL has been indicated, facilitates stone fragmentation in all cases, which is not achieved in up to 17% of the patients who did not use HCA. We recommend the use of HCA in patients prior to shock wave treatment to improve their fragmentation in calcium stones.
Using beneficial microorganisms along with sustainable strategies such as agronomic biofortification offers eco-friendly alternatives to combat climate change in ecosystems like dehesas. This study ...analyzes the combined effects of four wild Trichoderma spp. isolated from Extremadura, Spain (T. koningiopsis, two T. gamsii, and T. koningii, with negative and positive controls) and four Zn biofortification treatments (no Zn application; soil application of 5 mg of ZnSO4·7H2O per kg of soil, labeled soil Zn; two foliar applications of 5 mL 0.5% ZnSO4·7H2O, labeled foliar Zn; and soil + foliar combination, labeled SF) on Trifolium subterraneum performance. The combination of T. koningiopsis and T. gamsii with foliar Zn improved plant growth by up to 34.4%. Zinc accumulation was about 30% higher when T. gamsii and T. koningii were applied with SF, and their inoculation resulted in a 2.5-fold increase in ash. Trichoderma spp. affected nodulation differently; both T. gamsii inhibited nodulation by 24%, whereas neither T. koningiopsis nor T. koningii showed differences from the controls. These results highlight the potential of combining beneficial microorganisms with biofortification strategies to address nutrient deficiencies and improve agricultural sustainability. However, the complex interactions between both factors underscore the importance of strain selection and call for further research to optimize application strategies and elucidate underlying mechanisms.
Background: Paravalvular leak occurs in 5–17% of patients following surgical valve replacement, more often in mitral position. The prognosis without treatment is poor. Percutaneous device closure ...represents an alternative to repeat surgery. The objective of this work is to evaluate the medium and long-term results in the percutaneous closure of PVL in mitral prosthesis. Methods: This observational study is based on a retrospective registry including consecutive mitral PVL cases undergoing percutaneous closure at a single tertiary-care center from April 2010 to December 2020. The safety and efficacy results of the procedure, at 90 days and in the long term, were analyzed. Also, predictors of procedure failure and long-term events were identified. Results: A total of 128 consecutive mitral paravalvular leak closure procedures were included. Technical success was achieved in 115 (89.8%) procedures. The presence of multiple PVLs was the sole factor that independently predicted procedural failure. Median follow-up of our sample was 41.8 months (mean 47.7 ± 35.7 months). Underlying hemolytic anemia as the indication for PVL closure, a recent admission for decompensated HF, and lack of improvement in functional class emerged as consistent predictors of MACE and death during long-term follow-up, while lack of procedural success during the first PVL procedure and chronic kidney disease were also associated with MACE during follow-up. Conclusions: Percutaneous mitral PVL closure displayed high technical and procedural success rates, with an acceptable safety profile, in a high-risk population. Percutaneous mitral PVL closure achieved an improvement in short- and long-term functional class and a reduction of hemolysis in the vast majority of patients. In addition, long-term survival in our study was good, in particular for patients undergoing successful PVL closure procedures.
We analyzed the species distribution and susceptibility patterns of 433 strains of Aspergillus spp. isolated from respiratory samples of 419 in-patients included in multicenter prospective study ...(FUNGAE-IFI) between July 2014 and October 2015. Identification was carried out by conventional methods at each participating center and by molecular sequencing of a portion of the β-tubulin gene at one of the centers. In vitro susceptibility was evaluated by broth microdilution methods and using the E-test (for cryptic species). Species identified included 249 A. fumigatus sensu stricto, 60 A. terreus sensu stricto, 47 A. flavus sensu stricto, 44 A. tubingensis, 18 A. niger sensu stricto , five A. nidulans sensu stricto, three A. tamarii, two A. calidoustus, two A. carneus, one A. acuelatus, one A. carbonarius, and one A. sydowii. Cryptic species were found in 12.5% of isolates (n = 54). The frequency of non-wild-type isolates for amphotericin B was 3.4% (n = 15) of the isolates tested and for azoles 3% (n = 10). None of the Aspergillus spp. were non-wild type to echinocandins. Of the 54 cryptic species only two strains were non-wild-type strains by microdilution method (3.7%) (two A. tubingensis, one to amphotericin B and another one to voriconazole) and by E-test method five strains of A. tubingensis showed high minimal inhibitory concentration (MIC) to amphotericin B (11.4%) and five to azoles (12.1%), one A. calidoustus strain showed high MICs for three azoles (50%), A. carneus to itraconazole (100%) and A. sydowii to amphotericin B and itraconazole (100%). These results provide relevant information on susceptibility patterns, frequency, and epidemiology of species involved in respiratory tract samples and of the incidence of recently described cryptic species.
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