Ovaj rad objedinjuje i stavlja u međusobni odnos rezultate ispitivanja stavova dvije različite grupe ispitanika prema nedrvnim šumskim uslugama i proizvodima, služeći se metodom triangulacije (Denzin ...1978, Denzin i Lincoln 1998, 2003 i 2005). Podaci su prikupljeni pomoću dva dijelom ista upitnika, s time da se s lokalnim stanovništvom razgovaralo licem-u-lice, dok su stanovnici Karlovca svoj upitnik dobili poštom. Uz to je ocijenjena povoljnost šuma područja Petrove gore za pružanje rekreacijskih i krajobraznih usluga.
Rezultati ukazuju na visok stupanj slaganja stavova ispitanika ove dvije grupe te prepoznavanje važnosti ekološke i rekreacijske usluge šuma. Također je zanimljivo da obje grupe ispitanika vrlo rado i često odlaze u šumu, najčešće radi odmora i rekreacije, kao i zdravlja, a prilikom boravka u šumi gotovo redovno sakupljaju razne plodove i proizvode.
Uglavnom su zadovoljni stanjem šuma, no to zadovoljstvo opada povećanjem stupnja obrazovanja, ali nisu zadovoljni stanjem i održavanjem postojećih sadržaja u šumi. Također im nedostaju sadržaji prilagođeni osobama s posebnim potrebama.
Izuzetno dobru povoljnost cijeloga područja Petrove gore za pružanjem rekreacijskih i krajobraznih usluga može se bolje koristiti u promidžbene svrhe samog rekreacijskog centra, ali i kao dobru podlogu za privatno poduzetništvo. Obje grupe su prepoznale važnost šumskih plodova i iskazale interes za njihovim sakupljanjem te korištenjem, ponajprije za osobne svrhe.
Za dobivanje potpunije slike, potrebno je provesti dodatna istraživanja s posebnom pozornošću usmjerenom na posjetitelje šume.
The limited number of medicinal products available to treat of fungal infections makes control of fungal pathogens problematic, especially since the number of fungal resistance incidents increases. ...Given the high costs and slow development of new antifungal treatment options, repurposing of already known compounds is one of the proposed strategies. The objective of this study was to perform in vitro experimental tests of already identified lead compounds in our previous in silico drug repurposing study, which had been conducted on the known Drugbank database using a seven-step procedure which includes machine learning and molecular docking. This study identifies siramesine as a novel antifungal agent. This novel indication was confirmed through in vitro testing using several yeast species and one mold. The results showed susceptibility of Candida species to siramesine with MIC at concentration 12.5 µg/mL, whereas other candidates had no antifungal activity. Siramesine was also effective against in vitro biofilm formation and already formed biofilm was reduced following 24 h treatment with a MBEC range of 50–62.5 µg/mL. Siramesine is involved in modulation of ergosterol biosynthesis in vitro, which indicates it is a potential target for its antifungal activity. This implicates the possibility of siramesine repurposing, especially since there are already published data about nontoxicity. Following our in vitro results, we provide additional in depth in silico analysis of siramesine and compounds structurally similar to siramesine, providing an extended lead set for further preclinical and clinical investigation, which is needed to clearly define molecular targets and to elucidate its in vivo effectiveness as well.
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