Host behavior can affect host-pathogen dynamics, and sociality is predicted to increase risk of pathogen exposure. Many species minimize costs of parasitism by only aggregating seasonally, such as ...during reproductive periods, but colonial species may still be limited in their potential to evade pathogens. Bats are among the most gregarious mammals and females of many temperate species form maternity colonies in summer where they communally raise pups in both natural and anthropogenic roost structures. Social network structure may differ between natural and anthropogenic roosts in ways that affect pathogen dynamics. We used social network analysis to quantify interactions of big brown bats (Eptesicus fuscus) in a tree-roosting colony, where the colony is divided among multiple trees each day, and a building colony, where most of the colony roosts together each day. We simulated transmission of a pathogen throughout both sets of networks. We tested three hypotheses: (1) network metrics differ between pregnancy and lactation; (2) changing network structure between reproductive stages influences predicted pathogen dynamics; and (3) network metrics and predicted pathogen dynamics differ between colonies of bats in trees versus buildings. Network structure was weaker for bats roosting in trees during pregnancy and lactation compared to bats roosting in a building, and our models showed that a hypothetical pathogen would spread more rapidly for bats in the building colony. Our results are important for understanding variation in social tendencies and pathogen transmission among colonies of bats and have implications for conservation and public health. SIGNIFICANCE STATEMENT: Host behavior, particularly social behavior, can affect dynamics of wildlife pathogens. Bats are highly social mammals and females of temperate species form colonies in spring and early summer in tree or building roosts. Thermal characteristics of trees and buildings appear to differ in ways that affect roosting behavior and social interactions. We used social network analyses to quantify interactions of big brown bats in tree and building roosts and simulated consequences for pathogen dynamics. Network structure was weaker for bats roosting in trees with more frequent roost switching and relatively diffuse contacts across the network. Our models showed that a hypothetical pathogen could spread up to four times faster in a building colony compared to a colony of bats roosting in trees. Our results are important for understanding how sociality can influence pathogen dynamics in bats and have implications for conservation and public health.
To determine whether visual field (VF) loss is associated with lower levels of accelerometer-defined walking or physical activity in a nationally representative sample of American adults.
...Cross-sectional study.
A total of 2934 adults aged 40 years or older who participated in the examination component of the 2005-2006 National Health and Nutritional Examination Survey.
Frequency-doubling technology (FDT) testing was performed in both eyes and used to categorize subjects as having no VF loss, unilateral VF loss, or bilateral VF loss. Accelerometer data were collected over 7 days of normal activity.
Steps per day and daily minutes of moderate or vigorous physical activity (MVPA).
A total of 1468 participants (50.0%) had complete FDT and accelerometer data. Individuals without VF loss averaged 9751 steps/day and 20.8 minutes/day of MVPA, compared with 8023 steps/day and 14.5 minutes/day for subjects with unilateral VF loss (age-adjusted P = 0.11 and P = 0.51) and 6840 steps/day and 10.1 minutes/day for subjects with bilateral VF loss (age-adjusted P = 0.02 and 0.09, respectively). In multivariable models adjusted for age, sex, race/ethnicity, education, and several comorbid illnesses, individuals with bilateral VF loss took 17% fewer steps per day (P < 0.01) and engaged in 30% less MVPA (P = 0.02) than individuals without VF loss. No significant difference in steps per day or MVPA was observed between individuals with unilateral VF loss and no VF loss (P > 0.05). In addition to VF loss, older age, female sex, arthritis, diabetes, congestive heart failure (CHF), and stroke were significantly associated with fewer daily steps and minutes of MVPA (P < 0.05).
Bilateral VF loss is associated with less walking and physical activity in American adults. Patients with bilateral VF loss should be encouraged to engage safely in more physical activity.
The author(s) have no proprietary or commercial interest in any materials discussed in this article.
Context. Methyl isocyanide (CH3NC) is the isocyanide with the largest number of atoms confirmed in the interstellar medium (ISM), but it is not an abundant molecule, having only been detected towards ...a handful of objects. Conversely, its isomer, methyl cyanide (CH3CN), is one of the most abundant complex organic molecules detected in the ISM, with detections in a variety of low- and high-mass sources. Aims. The aims of this work are to determine the abundances of methyl isocyanide in the solar-type protostellar binary IRAS 16293–2422 and to understand the stark abundance differences observed between methyl isocyanide and methyl cyanide in the ISM. Methods. We use Atacama Large Millimeter/submillimeter Array (ALMA) observations from the Protostellar Interferometric Line Survey (PILS) to search for methyl isocyanide and compare its abundance with that of its isomer methyl cyanide. We use a new line catalogue from the Cologne Database for Molecular Spectroscopy (CDMS) to identify methyl isocyanide lines. We also model the chemistry with an updated version of the three-phase chemical kinetics model MAGICKAL, presenting the first chemical modelling of methyl isocyanide to date. Results. We detect methyl isocyanide for the first time in a solar-type protostar, IRAS 16293–2422 B, and present upper limits for its companion protostar, IRAS 16293–2422 A. Methyl isocyanide is found to be at least 20 times more abundant in source B compared to source A, with a CH3CN/CH3NC abundance ratio of 200 in IRAS 16293–2422 B and >5517 in IRAS 16293–2422 A. We also present the results of a chemical model of methyl isocyanide chemistry in both sources, and discuss the implications for methyl isocyanide formation mechanisms and the relative evolutionary stages of both sources. The chemical modelling is unable to match the observed CH3CN/CH3NC abundance ratio towards the B source at densities representative of that source. The modelling, however, is consistent with the upper limits for the A source. There are many uncertainties in the formation and destruction pathways of methyl isocyanide, and it is therefore not surprising that the initial modelling attempts do not reproduce observations. In particular, it is clear that some destruction mechanism of methyl isocyanide that does not destroy methyl cyanide is needed. Furthermore, these initial model results suggest that the final density plays a key role in setting the abundance ratio. The next steps are therefore to obtain further detections of methyl isocyanide in more objects, as well as undertaking more detailed physico-chemical modelling of sources such as IRAS16293.
Synopsis White-nose syndrome (WNS) has caused alarming declines of North American bat populations in the 5 years since its discovery. Affected bats appear to starve during hibernation, possibly ...because of disruption of normal cycles of torpor and arousal. The importance of hydration state and evaporative water loss (EWL) for influencing the duration of torpor bouts in hibernating mammals recently led to "the dehydration hypothesis," that cutaneous infection of the wing membranes of bats with the fungus Geomyces destructans causes dehydration which in turn, increases arousal frequency during hibernation. This hypothesis predicts that uninfected individuals of species most susceptible to WNS, like little brown bats (Myotis lucifugus), exhibit high rates of EWL compared to less susceptible species. We tested the feasibility of this prediction using data from the literature and new data quantifying EWL in Natterer's bats (Myotis nattereri), a species that is, like other European bats, sympatric with G. destructans but does not appear to suffer significant mortality from WNS. We found that little brown bats exhibited significantly higher rates of normothermic EWL than did other bat species for which comparable EWL data are available. We also found that Natterer's bats exhibited significantly lower rates of EWL, in both wet and dry air, compared with values predicted for little brown bats exposed to identical relative humidity (RH). We used a population model to show that the increase in EWL required to cause the pattern of mortality observed for WNS-affected little brown bats was small, equivalent to a solitary bat hibernating exposed to RH of ∼95%, or clusters hibernating in ∼87% RH, as opposed to typical near-saturation conditions. Both of these results suggest the dehydration hypothesis is plausible and worth pursuing as a possible explanation for mortality of bats from WNS.
Metal–organic frameworks with unsaturated metal centers in their crystal structures, such as Ni/DOBDC and Mg/DOBDC, are promising adsorbents for carbon dioxide capture from flue gas due to their high ...CO2 capacities at subatmospheric pressures. However, stability is a critical issue for their application. In this paper, the stabilities of Ni/DOBDC and Mg/DOBDC are investigated. Effects of steam conditioning, simulated flue gas conditioning, and long-term storage on CO2 adsorption capacities are considered. Results show that Ni/DOBDC can maintain its CO2 capacity after steam conditioning and long-term storage, whereas Mg/DOBDC does not. Nitrogen isotherms for Mg/DOBDC show a drop in surface area after steaming, corresponding to the decrease in CO2 adsorption, which may be caused by a reduction of unsaturated metal centers in its structure. Conditioning with dry simulated flue gas at room temperature only slightly affects CO2 adsorption in Ni/DOBDC. However, introducing water vapor into the simulated flue gas further reduces the CO2 capacity of Ni/DOBDC.
Structural flexibility in germline gene-encoded antibodies allows promiscuous binding to diverse antigens. The binding affinity and specificity for a particular epitope typically increase as antibody ...genes acquire somatic mutations in antigen-stimulated B cells. In this work, we investigated whether germline gene-encoded antibodies are optimal for polyspecificity by determining the basis for recognition of diverse antigens by antibodies encoded by three VH gene segments. Panels of somatically mutated antibodies encoded by a common VH gene, but each binding to a different antigen, were computationally redesigned to predict antibodies that could engage multiple antigens at once. The Rosetta multi-state design process predicted antibody sequences for the entire heavy chain variable region, including framework, CDR1, and CDR2 mutations. The predicted sequences matched the germline gene sequences to a remarkable degree, revealing by computational design the residues that are predicted to enable polyspecificity, i.e., binding of many unrelated antigens with a common sequence. The process thereby reverses antibody maturation in silico. In contrast, when designing antibodies to bind a single antigen, a sequence similar to that of the mature antibody sequence was returned, mimicking natural antibody maturation in silico. We demonstrated that the Rosetta computational design algorithm captures important aspects of antibody/antigen recognition. While the hypervariable region CDR3 often mediates much of the specificity of mature antibodies, we identified key positions in the VH gene encoding CDR1, CDR2, and the immunoglobulin framework that are critical contributors for polyspecificity in germline antibodies. Computational design of antibodies capable of binding multiple antigens may allow the rational design of antibodies that retain polyspecificity for diverse epitope binding.
To estimate the prevalence of Alzheimer's disease (AD) and other dementias in the USA using a nationally representative sample.
The Aging, Demographics, and Memory Study sample was composed of 856 ...individuals aged 71 years and older from the nationally representative Health and Retirement Study (HRS) who were evaluated for dementia using a comprehensive in-home assessment. An expert consensus panel used this information to assign a diagnosis of normal cognition, cognitive impairment but not demented, or dementia (and dementia subtype). Using sampling weights derived from the HRS, we estimated the national prevalence of dementia, AD and vascular dementia by age and gender.
The prevalence of dementia among individuals aged 71 and older was 13.9%, comprising about 3.4 million individuals in the USA in 2002. The corresponding values for AD were 9.7% and 2.4 million individuals. Dementia prevalence increased with age, from 5.0% of those aged 71-79 years to 37.4% of those aged 90 and older.
Dementia prevalence estimates from this first nationally representative population-based study of dementia in the USA to include subjects from all regions of the country can provide essential information for effective planning for the impending healthcare needs of the large and increasing number of individuals at risk for dementia as our population ages.
Structural restrictions are present even in the most sequence diverse portions of antibodies, the complementary determining region (CDR) loops. Previous studies identified robust rules that define ...canonical structures for five of the six CDR loops, however the heavy chain CDR 3 (HCDR3) defies standard classification attempts. The HCDR3 loop can be subdivided into two domains referred to as the "torso" and the "head" domains and two major families of canonical torso structures have been identified; the more prevalent "bulged" and less frequent "non-bulged" torsos. In the present study, we found that Rosetta loop modeling of 28 benchmark bulged HCDR3 loops is improved with knowledge-based structural restraints developed from available antibody crystal structures in the PDB. These restraints restrict the sampling space Rosetta searches in the torso domain, limiting the φ and ψ angles of these residues to conformations that have been experimentally observed. The application of these restraints in Rosetta result in more native-like structure sampling and improved score-based differentiation of native-like HCDR3 models, significantly improving our ability to model antibody HCDR3 loops.
Milton and Willis Milton, G.W., Willis, J.R., 2007. On modifications of Newton’s second law and linear continuum elastodynamics. Proc. R. Soc. A 463, 855–880 have recently discussed the general form ...of constitutive relations that should apply for the description of the dynamics of media with microstructure below the scale of measurement, and concluded that these relations should have the form, somewhat generalized, of effective relations for composites implied by a formulation of Willis Willis, J.R., 1981. Variational and related methods for the overall properties of composites. In: Yih, C.S. (Ed.), Advances in Applied Mechanics, vol. 21. Academic Press, New York, pp. l–78; Willis, J.R., 1981. Variational principles for dynamic problems for inhomogeneous elastic media, Wave Motion 3, 1–11. Whereas for a general composite (treated as a random medium), these relations can only be found at some level of approximation, they can be found exactly for a laminate with periodic microstructure, restricted to one-dimensional propagation normal to the lamination, for which Floquet theory is available. Such a medium becomes random if it is randomly translated: the exact position of any one interface is then not known, and ensemble averages may correspondingly be calculated. Example calculations demonstrate, completely explicitly, that “effective modulus” and “effective density” are operators, non-local in space and time; furthermore, if the medium does not display symmetry under reflection, mean stress is coupled not only to mean strain but also to mean velocity, and mean momentum density is likewise coupled not only to mean velocity but also to mean strain. A precisely similar structure applies to the propagation of electromagnetic waves: the effective medium is non-local and bi-anisotropic. The same structure is preserved under a generalized formulation, applicable to cases where only some component of the microstructure is observable.
Holter monitoring in dogs: 24 h vs. 48 h Mavropoulou, A.; Oliveira, P.; Willis, R.
The veterinary journal (1997),
June 2021, 2021-Jun, 2021-06-00, 20210601, Volume:
272
Journal Article
Peer reviewed
•Holter monitoring in dogs is useful for the detection and assessment of arrhythmias.•In 25% of cases, Holter diagnosed an arrhythmia that caused clinical signs.•A 48 h Holter increased the ...likelihood of documenting an arrhythmia during an event.•A 48 h Holter only slightly increased the likelihood of identifying an arrhythmia.
Holter monitoring has an important role in the diagnosis of cardiac arrhythmias in dogs with underlying heart disease or clinical signs such as intermittent weakness/ collapse or exercise intolerance, and in the assessment of antiarrhythmic treatment efficacy. A typical recording lasts for 24 h, although 48 h or any number of days up to 7 are possible, especially when investigating clinical signs that may not happen during the first 24 h. The objective of this study was to review retrospectively a large number of 48 h Holter recordings obtained from dogs to assess the possible incremental diagnostic yield of the second 24 h period in comparison to the first 24 h.
Three hundred and fifty four 48 h Holter recordings were included in the study for analysis. A 48 h Holter recording contributed to a 14.5% increase in the likelihood of documenting the cardiac rhythm during an event of interest; a 24 h recording increased the diagnostic yield from 32.2% to 46.7%. When the recordings were grouped according to the most important rhythm abnormalities (supraventricular arrhythmias group, ventricular arrhythmias group, bradyarrhythmias group, no arrhythmias group), the 48 h Holter monitor contributed to a 5% increase in the likelihood of identifying a relevant cardiac rhythm abnormality, increasing the diagnostic yield from 58% to 63%. This benefit occurred mostly in dogs with iterative or paroxysmal supraventricular arrhythmias. In dogs with bradyarrhythmias or ventricular arrhythmias, an additional 24 h of analysis was useful in only a limited number of cases.
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