Atmospheric particulate matter is a wide-ranging environmental pollutant that can cause serious harm and poses a serious threat to public health. In this study, the near-surface particulate matter ...removal ability was quantitatively analyzed and compared for different land types under different pollution levels. The results showed that the concentrations of particulate matter 10 μm or less in diameter (PM10) and 2.5 μm or less in diameter (PM2.5) were higher in the morning and lower in the afternoon and that the seasonal variation was autumn > winter > spring > summer at a forest site. The diurnal concentration of particulate matter at a wetland site decreased continuously, with a seasonal variation of winter > autumn > spring > summer. The annual variation in the particulate matter concentration was higher in 2017 than in 2016 at both the forest and wetland sites. Forests remove particulate matter via plant leaves and root absorption, and wetlands rely on the enhancement of the relative air humidity to promote the absorption and accumulation of particles. For different air pollution levels, the deposition flux of PM2.5 increased with the pollution gradient. For the same air quality pollution level, the deposition flux of PM2.5 at the forest site was approximately 1.29 times higher than that at the wetland site. Data concerning PM10 in forests and wetlands are lacking. The results show that the deposition effect of the forest on particulate matter was better than that of the wetland.
Drought stress is one of the most impactful abiotic stresses to global wheat production. Therefore, identifying key regulators such as the calcineurin B-like protein interacting protein kinase (CIPK) ...in the signaling cascades known to coordinate developmental cues and environmental stimuli represents a useful approach to improve drought tolerance. However, functional studies have been very limited partly due to the difficulties in prioritizing candidate genes from the large TaCIPK family. To address this issue, we demonstrate a straight-forward strategy by analyzing gene expression patterns in response to phytohormones or stresses and identified TaCIPK19 as a new regulator to improve drought tolerance. The effects of TaCIPK19 on drought tolerance were evaluated in both tobacco and wheat through transgenic approach. Ectopic expression of TaCIPK19 in tobacco greatly improves drought tolerance with enhanced ABA biosynthesis/signaling and ROS scavenging capacity. TaCIPK19 overexpression in wheat also confers the drought tolerance at both seedling and mature stages with enhanced ROS scavenging capacity. Additionally, potential CBL partners interacting with TaCIPK19 were investigated. Collectively, our finding exemplifies a straight-forward approach to facilitate reverse genetics related to abiotic stress improvement and demonstrates TaCIPK19 as a new candidate gene to improve ROS scavenging capacity and drought tolerance, which is useful for genetic improvement and breeding application in wheat.
•A straight-forward strategy to prioritize functional studies is proposed for large gene families.•TaCIPK19 overexpression in tobacco enhances ABA sensitivity and reduces water loss.•TaCIPK19 improves the drought tolerance in plants by enhancing the ROS scavenging capacity.
Lung cancer is responsible for the leading cause of cancer-related death worldwide, which lacks effective therapies. In recent years, accumulating evidence on the understanding of the antitumor ...activity of the immune system has demonstrated that immunotherapy is one of the powerful alternatives in lung cancer therapy. T cells are the core of cellular immunotherapy, which are critical for tumorigenesis and the treatment of lung cancer. Based on the different expressions of surface molecules and functional points, T cells can be subdivided into regulatory T cells, T helper cells, cytotoxic T lymphocytes, and other unconventional T cells, including γδ T cells, nature killer T cells and mucosal-associated invariant T cells. Advances in our understanding of T cells' functional mechanism will lead to a number of clinical trials on the discovery and development of new treatment strategies. Thus, we summarize the biological functions and regulations of T cells on tumorigenesis, progression, metastasis, and prognosis in lung cancer. Furthermore, we discuss the current advancements of technologies and potentials of T-cell-oriented therapeutic targets for lung cancer.
Nitrogen dioxide (NO2) is one of the most dangerous air pollutants that can affect human health even at the ppb (part per billion) level. Thus, the superior sensing performance of nitrogen dioxide ...gas sensors is an imperative for real-time environmental monitoring. Traditional solid-state sensors based on metal-oxide transistors have the drawbacks of high power consumption, high operating temperature, poor selectivity, and difficult integration with other electronics. In that respect, graphene-based gas sensors have been extensively studied as potential replacements. However, their advantages of high sensing efficiency, low power consumption, and simple electronic integration have been countered by their slow response and poor repeatability. Here, we report the fabrication of high-performance ultraviolet (UV)-assisted room temperature NO2 sensors based on chemical vapor deposition-grown graphene. UV irradiation improves the response of the sensor sevenfold with respect to the dark condition attaining 26% change in resistance at 100 ppm NO2 concentration with a practical detection limit below 1 ppm (42.18 ppb). In addition, the recovery time was shortened fivefold to a few minutes and the excellent repeatability. This work may provide a promising and practical method to mass produce room-temperature NO2 gas sensors for real-time environment monitoring due to its simple fabrication process, low cost, and practicality.
Oil spill has led to severe environmental and ecological problems. Due to the harsh environmental conditions, the bioremediation technology is not successfully used to remedy the oil spill in marine ...environment. In this study, immobilization technology was used to immobilize bacteria on natural organic carriers (i.e., wood chips and maize straw). The higher surface area of in wood chips leads to larger biomass density (0.0242 gVSS/g) than that of maize straw of 0.0097 gVSS/g carrier. Compared with biodegradation efficiency of free bacteria (44.79%), the immobilized bacteria on wood chips and maize straw reached to 73.39% and 52.28%, respectively. The high biological activity of the immobilized bacteria can be also explained by nutrients, such as TN (total nitrogen) and TP (total phosphorus), released from wood chips and maize straw, which was 8.83 mg/g and 5.53 mg/g, 0.0624 mg/g and 0.0099 mg/g, respectively.
Iron is important for a remarkable array of essential functions during brain development, and it needs to be provided in adequate amounts, especially to preterm infants. In this review article, we ...provide an overview of iron metabolism and homeostasis at the cellular level, as well as its regulation at the mRNA translation level, and we emphasize the importance of iron for brain development in fetal and early life in preterm infants. We also review the risk factors for disrupted iron metabolism that lead to high risk of developing iron deficiency and subsequent adverse effects on neurodevelopment in preterm infants. At the other extreme, iron overload, which is usually caused by excess iron supplementation in iron-replete preterm infants, might negatively impact brain development or even induce brain injury. Maintaining the balance of iron during the fetal and neonatal periods is important, and thus iron status should be monitored routinely and evaluated thoroughly during the neonatal period or before discharge of preterm infants so that iron supplementation can be individualized.
Nanothermometers enable the detection of temperature changes at the microscopic scale, which is crucial for elucidating biological mechanisms and guiding treatment strategies. However, temperature ...monitoring of micron-scale structures in vivo using luminescent nanothermometers remains challenging, primarily due to the severe scattering effect of biological tissue that compromises the imaging resolution. Herein, a lanthanide luminescence nanothermometer with a working wavelength beyond 1500 nm is developed to achieve high-resolution temperature imaging in vivo. The energy transfer between lanthanide ions (Er
and Yb
) and H
O molecules, called the environment quenching assisted downshifting process, is utilized to establish temperature-sensitive emissions at 1550 and 980 nm. Using an optimized thin active shell doped with Yb
ions, the nanothermometer's thermal sensitivity and the 1550 nm emission intensity are enhanced by modulating the environment quenching assisted downshifting process. Consequently, minimally invasive temperature imaging of the cerebrovascular system in mice with an imaging resolution of nearly 200 μm is achieved using the nanothermometer. This work points to a method for high-resolution temperature imaging of micron-level structures in vivo, potentially giving insights into research in temperature sensing, disease diagnosis, and treatment development.
In-person social events bring people to places, while people and places influence where and what social events occur. Knowing what people do and where they build social relationships gives insights ...into the distribution and availability of places for social functions. We developed a Bayesian Network model, integrating points of interest (POIs) and sociodemographic characteristics, to estimate the probabilistic effects of places and people on the presence of social events. A case study in Dallas demonstrated the utility and performance of the model. The Bayesian Network model predicted the presence likelihoods for seven types of social events with an R2 value around 0.83 (95% confidence interval). For both the presence and absence of social events at locations, the model predictions were within a 20% error for most event types. Furthermore, the model suggested POI, age, education, and population density configurations as important contextual variables for place–event associations across locations. A spatial cluster analysis identified likely multifunctional hotspots for social events (i.e., socially vibrant places). While psychological and cultural factors likely contribute further to local likelihoods of social event occurrences, the proposed conceptually informed geospatial data-science approach elucidated intricate place–people–event relationships and implicates inclusive, participatory places for urban development.
Structures of peptide-loaded major histocompatibility complex class I (pMHC-I) and class II (pMHC-II) complexes are similar. However, whereas pMHC-II complexes include similar-sized IIα and IIβ ...chains, pMHC-I complexes include a heavy chain (HC) and a single domain molecule β
2
-microglobulin (β
2
-m). Recently, we elucidated several pMHC-I and pMHC-II structures of primitive vertebrate species. In the present study, a comprehensive comparison of pMHC-I and pMHC-II structures helps to understand pMHC structural evolution and supports the earlier proposed—though debated—direction of MHC evolution from class II-type to class I. Extant pMHC-II structures share major functional characteristics with a deduced MHC-II-type homodimer ancestor. Evolutionary establishment of pMHC-I presumably involved important new functions such as (i) increased peptide selectivity by binding the peptides in a closed groove (ii), structural amplification of peptide ligand sequence differences by binding in a non-relaxed fashion, and (iii) increased peptide selectivity by syngeneic heterotrimer complex formation between peptide, HC, and β
2
-m. These new functions were associated with structures that since their establishment in early pMHC-I have been very well conserved, including a shifted and reorganized P1 pocket (aka A pocket), and insertion of a β
2
-m hydrophobic knob into the peptide binding domain β-sheet floor. A comparison between divergent species indicates better sequence conservation of peptide binding domains among MHC-I than among MHC-II, agreeing with more demanding interactions within pMHC-I complexes. In lungfishes, genes encoding fusions of all MHC-IIα and MHC-IIβ extracellular domains were identified, and although these lungfish genes presumably derived from classical MHC-II, they provide an alternative mechanistic hypothesis for how evolution from class II-type to class I may have occurred.
Generic object detection is a crucial task for autonomous driving. To devise a safe and efficient object detector, the following aspects are required to be considered: high accuracy, real‐time ...inference speed and small model size. Herein, a simple yet effective anchor‐free object detector named L4Net is proposed, which incorporates a keypoint detection backbone and a co‐attention scheme into a unified framework, and achieves lower computation cost with higher detection accuracy than prior art across a wide spectrum of resource constrains. Specifically, the backbone utilizes Multi‐scale Receptive‐fields Enhancement module (MRE) to capture context‐wise information, where the features of object scale and shape invariance are simultaneously considered. The co‐attention scheme integrates the strength of both Class‐agnostic Attention (CA) and Semantic Attention (SA), and explores the valuable features from low‐level to high‐level to generate more accurate prediction boxes. Compared with previous feature fusion strategy, multi‐scale features are selectively integrated by fully exploiting the different characteristics of low‐level and high‐level features, which leads to a small model size and faster inference speed. Extensive experiments on four well‐known datasets demonstrate the effectiveness of our method. For instance, L4Net achieves 71.68% mAP on KITTI test set, with 13.7 M model size at the speed of 149 FPS on NVIDIA TX and 30.7 FPS on Qualcomm‐based device, respectively, which is 4x smaller and 2x faster than baseline model.
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