•3D printing performance of wheat-based gels was affected by materials’ components.•Physicochemical and structural properties about the printed samples were assayed.•LF-NMR can be used to explore ...changes of water distribution during storage.
The effects of material composition on the quality of 3D-printed food using wheat starch, flour and whole meal were explored. Results showed that samples molded by wheat starch and whole meal obtained a more accurate and better quality printing than wheat flour. Wheat flour had the worst printable capacity as it gets stuck easily to the wall of the printer. Starch-protein complexes appeared in the samples of wheat flour and whole meal. Whole meal constituted protein, fat, fiber and starch to form various complexes, which strengthen the gel network cross-linking, retard starch aging significantly, and enhance the water binding capacity than just the starch only sample. Printed samples using whole meal still possess a soft and elastic texture after long-time storage. Overall, whole meal with higher protein, fat and fiber content was better for food 3D printing than wheat starch and flour.
Targeted imaging of cancer cells is crucial for early diagnosis. Mucin is a transmembrane protein that is overexpressed by cancer cells and is considered a cancer target. Specific recognition of ...mucin by aptamers has been receiving increasing attention in recent years. In this study, we use DNA MUC1 aptamer as a protective agent and target molecule in the synthesis of ultra-small fluorescent gold nanoclusters (MUC1-AuNCs) via a simple one-step method. MUC1-AuNCs exhibited red fluorescence emission with excellent stability over a wide pH range and under strong illumination. Confocal images showed that the synthesized MUC1-AuNCs efficiently targeted mucin overexpressing 4T1 cancer cells, but were not observed in 293T normal cells. Furthermore, the MUC1-AuNCs had a 5667 ns lifetime and 235 nm Stokes shifts and markedly eliminated background interference, suggesting they are a promising fluorescent probe for cell-targeted labeling and imaging.
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•Luminescent gold nanoclusters were synthesized by thiolated MUC1 DNA aptamer as template.•The MUC1-AuNCs demonstrate good stability, low toxicity and specific cancer cell targeting properties.•The MUC1-AuNCs with long fluorescence lifetimes and large stokes shift can reduce background interference.
A total of 151 recombinant inbred lines (RILs) were derived from the cross between ‘
Cucumis sativus
L.
hardwickii
’ (HW) and a cultivated Northern Chinese inbred line ‘XinTaiMiCi’ (XTMC). We used ...resequencing to construct the genetic map and analyze the genetic background of RIL population, and combined with the phenotypes of RIL population and the analysis of RNA-seq data, we located the major loci controlling the fruit length of cucumber and related analysis. A genetic map containing 600 bin markers was constructed via re-sequencing. Based on the phenotype data collected in two different seasons (spring 2021 and autumn 2022), the major quantitative trait loci (QTLs) controlling cucumber fruit length were located and their transcriptomic analysis carried out. The results revealed three QTLs (
Fl2.1
,
Fl4.1
, and
Fl6.1
) detected repeatedly in the two seasons, of which
Fl4.1
was the dominant QTL. From the functional annotation of corresponding genes there, we discovered the gene
Csa4G337340
encoding an auxin efflux carrier family protein. The expression of that gene was significantly lower in XTMC and the long-fruit RIL lines than in HW and the short-fruit RIL lines; hence, we speculated the gene could be negatively correlated with the fruit length of cucumber. Transcriptomic analysis showed that 259 differentially expressed genes (DEGs) were enriched in the plant hormone signal transduction pathway. In addition, among those DEGs, 509 transcription factors were detected, these distributed in several transcription factor gene families, such as bHLH, AP2/ErF -ERF, C2H2, and NAC. Therefore, we concluded that the major gene controlling the fruit length of cucumber is located in the interval of
Fl4.1
, whose gene
Csa4G337340
may be involved in the negative regulation of fruit length. Further, genes related to plant hormone signal transduction and several transcription factors were also found involved in the regulation of cucumber fruit length. Our results provide a reference for the fine mapping of major genes and analyzing the mechanism of cucumber fruit length.
ObjectiveTo investigate the effects of the willingness to live in elder care institutions associated with individual factors, family environment and the community environment in the elderly in ...China.DesignCross-sectional survey.SettingHeilongjiang Province, China.ParticipantsA total of 1003 elderly people were selected through multistage sampling in Heilongjiang Province.Primary and secondary outcome measuresA multistage, stratified sampling design was employed. Differences in health status, family environment and community environment of the respondents were compared with the t-test and χ2 test. Logistic regression analysis was performed to assess key determinants of willingness to live in institutions.ResultsThis study showed that 45.4% of respondents were willing to live in elder care institutions in the future. Factors influencing willingness to live in elder care institutions were age, house ownership, living with spouse and children, disease caregivers and availability of home healthcare services. The elders who had no property (OR=2.37, 95% CI 1.750 to 3.200, p<0.01) and those aged 80 or above (OR=2.25, 95% CI 1.490 to 3.400, p<0.01) were, respectively, 2.370 and 2.250 times more receptive to living in elder care institutions than their control groups. However, those living with a spouse (OR=0.47, 95% CI 0.287 to 0.762, p<0.01), living with children (OR=0.25, 95% CI 0.158 to 0.402, p<0.01) or living with a spouse and children (OR=0.29, 95% CI 0.160 to 0.509, p<0.01) were less willing to live in elder care institutions.ConclusionsThese results suggest that the willingness to enter elder care institutions is affected by individual, family environmental and community environmental factors. We should vigorously develop community-centred intensive home-based elder care services by improving the quality and availability of home health services by expanding investment in the community.
SLC3A2, the heavy chain of the CD98 protein, is highly expressed in many cancers, including lung cancer. It can regulate the proliferation and the metastasis of cancer cells via the integrin ...signaling pathway. Liquid biopsy is a novel method for tumor diagnosis. The diagnostic or prognostic roles of serum SLC3A2 in lung cancer are still not clear. In this study, we analyzed SLC3A2 mRNA levels in human lung squamous cell carcinoma (LUSC) and lung adenocarcinoma (LUAD) using the TCGA database and serum SLC3A2 protein levels using ELISA. We confirmed high SLC3A2 levels in both the serum and tissue of LUAD and LUSC patients. Both serum and tissue SLC3A2 could be used as prognostic markers for overall LUAD and subgroups of LUSC patients. SLC3A2 induced tumorigenesis via the MEK/ERK signaling pathway in LUAD and LUSC cells.
•Exposure to individual and mixed PFASs impacted thyroid hormones.•Sex and age-specific effects were observed in PFAS-related changes.•Critical exposure thresholds were identified.•Novel findings ...were found within a large, representative Chinese population.
Disruption of thyroid homeostasis has been indicated in human studies on the effects of per- and polyfluoroalkyl substances (PFASs). However, limited research exists on this topic within the general Chinese population. Based on a substantial and representative sample of the Chinese adult population, our study provides insight into how PFASs specifically affect thyroid homeostasis. The study included 10 853 participants, aged 18 years and above, sampled from nationally representative data provided by the China National Human Biomonitoring (CNHBM). Weighted multiple linear regression and restricted cubic spline (RCS) models were used to explore the associations between eight individual PFAS concentrations and total thyroxine (T4), total triiodothyronine (T3), and the T4/T3 ratio. Bayesian kernel machine regression (BKMR) and quantile-based g-computation (qgcomp) were employed to explore the joint and independent effects of PFASs on thyroid homeostasis. Both individual PFASs and PFAS mixtures exhibited a significant inverse association with serum T3 and T4 levels, and displayed a positive association with the T4/T3 ratio. Perfluoroundecanoic acid (PFUnDA) –0.07 (95 % confidence interval (CI): –0.08, –0.05) exhibited the largest change in T3 level. PFUnDA also exhibited a higher weight compared to other PFAS compounds in qgcomp models. Additionally, a critical exposure threshold for each PFAS was identified based on nonlinear dose–response associations; beyond these thresholds, the decreases in T3 and T4 levels plateaued. Specifically, for perfluoroheptane sulfonic acid (PFHpS) and 6:2 chlorinated polyfluorinated ether sulfonate (6:2 Cl-PFESA), an initial decline in hormone levels was observed, followed by a slight increase when concentrations surpassed 0.7 ng/mL and 2.5 ng/mL, respectively. Sex-specific effects were more pronounced in females, and significant associations were observed predominantly in younger age groups. These insights contribute to our understanding of how PFAS compounds impact thyroid health and emphasize the need for further research and environmental management measures to address these complexities.
Background:
Growing evidence suggests a bidirectional association between bipolar disorder (BD) and inflammatory bowel disease (IBD); however, observational studies are prone to confounding, making ...causal inference and directional determination of these associations difficult.
Methods:
We performed bidirectional two-sample Mendelian randomization (MR) and selected single nucleotide polymorphisms (SNPs) associated with BD and IBD as instrumental variables (IV). SNPs and genetic associations with BD and IBD were obtained from the latest genome-wide association studies (GWAS) in Europeans (BD: cases/controls: 20352/31358; IBD: 12882/21770; Crohn’s disease (CD): 5,956/14927; ulcerative colitis (UC): 6968/20464). The inverse-variance-weighted method was the major method used in MR analyses. MR-Egger, weight mode, simple mode, and weighted median were used for quality control.
Results:
Genetically predicted BD (per log-odds ratio increase) was significantly positively associated with risk of IBD (OR: 1.18, 95% CI: 1.04–1.33), and UC (OR = 1.19, 95% CI: 1.05–1.35), but not CD (OR = 1.18, 95% CI: 0.95–1.48). The validation analysis found that combined OR of IBD, CD, and UC increased per log-OR of BD were 1.16(95% CI: 1.02–1.31), 1.20(95% CI: 0.98–1.48) 1.17(95% CI: 1.02–1.35), respectively. In contrast, no causal relationship was identified between genetically influenced IBD and BD.
Conclusion:
Our results confirm a causal relationship between BD and IBD, which may influence clinical decisions on the management of BD patients with intestinal symptoms. Although the reverse MR results did not support a causal effect of IBD on BD, the effect of the IBD active period on BD remains to be further investigated.
In this paper, taking the generalized synchronization problem of discrete chaotic systems as a starting point, a generalized synchronization method incorporating error-feedback coefficients into the ...controller based on the generalized chaos synchronization theory and stability theorem for nonlinear systems is proposed. Two discrete chaotic systems with different dimensions are constructed in this paper, the dynamics of the proposed systems are analyzed, and finally, the phase diagrams, Lyapunov exponent diagrams, and bifurcation diagrams of these are shown and described. The experimental results show that the design of the adaptive generalized synchronization system is achievable in cases in which the error-feedback coefficient satisfies certain conditions. Finally, a chaotic hiding image encryption transmission system based on a generalized synchronization approach is proposed, in which an error-feedback coefficient is introduced into the controller.
The physical implementation of the continuous-time memristor makes it widely used in chaotic circuits, whereas the discrete-time memristor has not received much attention. In this paper, the ...backward-Euler method is used to discretize the TiO2 memristor model, and the discretized model also meets the three fingerprints characteristics of the generalized memristor. The short period phenomenon and uneven output distribution of one-dimensional chaotic systems affect their applications in some fields, so it is necessary to improve the dynamic characteristics of one-dimensional chaotic systems. In this paper, a two-dimensional discrete-time memristor model is obtained by linear coupling of the proposed TiO2 memristor model and one-dimensional chaotic systems. Since the two-dimensional model has infinite fixed points, the stability of these fixed points depends on the coupling parameters and the initial state of the discrete TiO2 memristor model. Furthermore, the dynamic characteristics of one-dimensional chaotic systems can be enhanced by the proposed method. Finally, we apply the generated chaotic sequence to secure communication.
Abstract
Placenta plays essential role in successful pregnancy, as the most important organ connecting and interplaying between mother and fetus. However, the cellular characteristics and molecular ...interaction of cell populations within the fetomaternal interface is still poorly understood. Here, we surveyed the single-cell transcriptomic landscape of human full-term placenta and revealed the heterogeneity of cytotrophoblast cell (CTB) and stromal cell (STR) with the fetal/maternal origin consecutively localized from fetal section (FS), middle section (Mid_S) to maternal section (Mat_S) of maternal–fetal interface. Then, we highlighted a subpopulation of CTB, named trophoblast progenitor-like cells (TPLCs) existed in the full-term placenta and mainly distributed in Mid_S, with high expression of a pool of putative cell surface markers. Further, we revealed the putative key transcription factor
PRDM6
that might promote the differentiation of endovascular extravillous trophoblast cells (enEVT) by inhibiting cell proliferation, and down-regulation of
PRDM6
might lead to an abnormal enEVT differentiation process in PE. Together, our study offers important resources for better understanding of human placenta and stem cell-based therapy, and provides new insights on the study of tissue heterogeneity, the clinical prevention and control of PE as well as the maternal–fetal interface.
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