Hydrogel fibers have attracted substantial interest for application in flexible electronics due to their ionic conductivity, high specific surface area, and ease of constructing multidimensional ...structures. However, universal continuous spinning methods for hydrogel fibers are yet lacking. Based on the hydrophobic mold induced regional heterogeneous polymerization, a universal self‐lubricating spinning (SLS) strategy for the continuous fabrication of hydrogel fibers from monomers is developed. The universality of the SLS strategy is demonstrated by the successful spinning of 10 vinyl monomer‐based hydrogel fibers. Benefiting from the universality of the SLS strategy, the SLS strategy can be combined with pre‐gel design and post‐treatment toughening to prepare highly entangled polyacrylamide (PAM) and ionic crosslinked poly(acrylamide‐co‐acrylic acid)/Fe3+ (W‐PAMAA/Fe3+) hydrogel fibers, respectively. In particular, the W‐PAMAA/Fe3+ hydrogel fiber exhibited excellent mechanical properties (tensile stress > 4 MPa, tensile strain > 400%) even after 120 days of swelling in the pH of 3–9. Furthermore, owing to the excellent multi‐faceted performance and one‐dimensionality of W‐PAMAA/Fe3+ hydrogel fibers, flexible sensors with different dimensions and functions can be constructed bottom‐up, including the one‐dimensional (1D) strain sensor, two‐dimensional (2D) direction sensor, three‐dimensional (3D) pressure sensor, and underwater communication sensor to present the great potential of hydrogel fibers in flexible electronics.
A universal self‐lubricating spinning strategy (SLS) is developed for the continuous fabrication of hydrogel fibers and its spinning mechanism is discussed. The high‐tough hydrogel fibers prepared based on the SLS strategy demonstrate the ability to construct flexible electronic devices from the bottom up.
Development of versatile theranostic agents that simultaneously integrate therapeutic and diagnostic features remains a clinical urgent. Herein, we aimed to prepare uniform PEGylated ...(lactic-co-glycolic acid) (PLGA) microcapsules (PB@(Fe3O4@PEG-PLGA) MCs) with superparamagnetic Fe3O4 nanoparticles embedded in the shell and Prussian blue (PB) NPs inbuilt in the cavity via a premix membrane emulsification (PME) method. On account of the eligible geometry and multiple load capacity, these MCs could be used as efficient multi-modality contrast agents to simultaneously enhance the contrasts of US, MR and PAT imaging. In-built PB NPs furnished the MCs with excellent photothermal conversion property and embedded Fe3O4 NPs endowed the magnetic location for fabrication of targeted drug delivery system. Notably, after further in-situ encapsulation of antitumor drug of DOX, (PB+DOX)@(Fe3O4@PEG-PLGA) MCs possessed more unique advantages on achieving near infrared (NIR)-responsive drug delivery and magnetic-guided chemo-photothermal synergistic osteosarcoma therapy. In vitro and in vivo studies revealed these biocompatible (PB+DOX)@(Fe3O4@PEG-PLGA) MCs could effectively target to the tumor tissue with superior therapeutic effect against the invasion of osteosarcoma and alleviation of osteolytic lesions, which will be developed as a smart platform integrating multi-modality imaging capabilities and synergistic effect with high therapy efficacy.
Schematic illustration of a theranostic agent of (PB+DOX)@(Fe3O4@PEG-PLGA) MCs towards multi-modality imaging, magnetic targeting ability and NIR-triggered chemo-photothermal osteosarcoma therapy. Display omitted
•A facile and efficient premix membrane emulsification method to prepare multifunctional theranostic agents.•These MCs possess excellent US/MR/PAT multi-modality imaging performance.•These MCs achieve the synergistic therapeutic outcomes of chemotherapy and photothermal therapy in response to NIR stimuli.•A combined effect of magnetic targeting and chemo-photothermal osteosarcoma therapy with prominent anticancer efficacy.
The goal of biometrics is to recognize humans based on their physical and behavioral characteristics. Preliminary studies have demonstrated that the electroencephalogram(EEG) is potentially more ...secure and private than traditional biometric identifiers. At present, the EEG identification method targets specific tasks and cannot be generalized. In this study, a novel EEG-based biometric identification method that extracts the phase synchronization (PS) features for subject identification is proposed under a variety of tasks. We quantified the PS features by the phase locking value (PLV) in different frequency bands. Subsequently, we employed the principal component analysis (PCA) to reduce the dimension. Then, we used the linear discriminant analysis (LDA) to construct a projection space and projected the features onto the projection space. Finally, a feature vector was assigned to the class label. The experimental results of the proposed method used on 3 datasets with different cognitive tasks showed high classification accuracies and relatively good stabilities. From the results, we found that particularly in the beta and gamma bands, the average accuracies are more than 97% with the standard deviation equal to or less than the magnitude 10e-2 for both Dataset 1 and Dataset 2. For Dataset 3, the PS feature vectors in all off the bands have high classification accuracies, which are more than 97% with the standard deviation of the same magnitude. Our work demonstrated that the phase synchronization of EEG signals has task-free biometric properties, which can be used for subject identification.
We reported a novel African swine fever virus (ASFV) strain that had a three-large-fragment deletion and unique variations in genome. This isolate displayed a nonhemadsorbing phenotype and had ...homogeneous proliferation compared with the wild-type ASFV strain. Our findings highlighted the urgent need for further investigation of ASFV variations in China.
African swine fever virus (ASFV) has been circulating in China for 5 years, and low virulent strains with changes in the genome have been reported. Nevertheless, there is still a lack of knowledge about the epidemic strains at the whole-genome level. This study reported a novel strain and further analyzed its genomic and biological characteristics. In addition, our study also suggests that whole-genome sequencing plays a key role in the epidemiology investigation of ASFV variations.
Adaptive thermogenesis is essential for survival, and therefore is tightly regulated by a central neural circuit. Here, we show that microRNA (miR)-33 in the brain is indispensable for adaptive ...thermogenesis. Cold stress increases miR-33 levels in the hypothalamus and miR-33
mice are unable to maintain body temperature in cold environments due to reduced sympathetic nerve activity and impaired brown adipose tissue (BAT) thermogenesis. Analysis of miR-33
dopamine-β-hydroxylase (DBH)-Cre mice indicates the importance of miR-33 in Dbh-positive cells. Mechanistically, miR-33 deficiency upregulates gamma-aminobutyric acid (GABA)
receptor subunit genes such as Gabrb2 and Gabra4. Knock-down of these genes in Dbh-positive neurons rescues the impaired cold-induced thermogenesis in miR-33
DBH-Cre mice. Conversely, increased gene dosage of miR-33 in mice enhances thermogenesis. Thus, miR-33 in the brain contributes to maintenance of BAT thermogenesis and whole-body metabolism via enhanced sympathetic nerve tone through suppressing GABAergic inhibitory neurotransmission. This miR-33-mediated neural mechanism may serve as a physiological adaptive defense mechanism for several stresses including cold stress.
Survivors of sepsis may encounter cognitive impairment following their recovery from critical condition. At present, there is no standardized treatment for addressing sepsis-associated ...encephalopathy.
GG (LGG) is a prevalent bacterium found in the gut microbiota and is an active component of probiotic supplements. LGG has demonstrated to be associated with cognitive improvement. This study explored whether LGG administration prior to and following induced sepsis could ameliorate cognitive deficits, and explored potential mechanisms.
Female C57BL/6 mice were randomly divided into three groups: sham surgery, cecal ligation and puncture (CLP), and CLP+LGG. Cognitive behavior was assessed longitudinally at 7-9d, 14-16d, and 21-23d after surgery using an open field test and novel object recognition test. The impact of LGG treatment on pathological changes, the expression level of brain-derived neurotrophic factor (BDNF), and the phosphorylation level of the TrkB receptor (p-TrkB) in the hippocampus of mice at two weeks post-CLP (16d) were evaluated using histological, immunofluorescence, immunohistochemistry, and western blot analyses.
The CLP surgery induced and sustained cognitive impairment in mice with sepsis for a minimum of three weeks following the surgery. Compared to mice subjected to CLP alone, the administration of LGG improved the survival of mice with sepsis and notably enhanced their cognitive functioning. Moreover, LGG supplementation significantly alleviated the decrease in hippocampal BDNF expression and p-TrkB phosphorylation levels caused by sepsis, preserving neuronal survival and mitigating the pathological changes within the hippocampus of mice with sepsis. LGG supplementation mitigates sepsis-related cognitive impairment in mice and preserves BDNF expression and p-TrkB levels in the hippocampus.
In order to avoid the unsafe operation and raise efficiency of yokeless and segmented armature axial flux motors at high speed, the control current of air gap flux is expected to be as small as ...possible with the same field-weakening effect. To reduce the control complexity, a new structure of module poles with a combination of permanent magnet and soft magnetic material is proposed, which has the characteristics of lower d-axis reluctance and a higher performance of yokeless and segmented armature axial flux motor with surface mounted permanent magnet. According to finite element analysis (FEA), the flux distributions of a rotor pole in no-load and demagnetization condition are contrasted, and under this new configuration, the derivative analytical models of back electromotive-force (EMF), electromagnetic torque, and air gap flux are validated, moreover, the influence of soft magnetic material of rotor poles on controlling the air gap flux is investigated in different load. Based on a particular objective function, the combination of permanent magnet and soft magnetic material is optimized. The results show that optimal solution of field-weakening performance of yokeless and segmented armature axial flux motors can be improved effectively and legitimately.
Anti-icing coatings on outdoor infrastructures inevitably suffer from mechanical injuries in numerous icing scenarios such as hailstorms, sandstorms, impacts of foreign objects, and icing-deicing ...cycles. Herein, the mechanisms of surface-defect-induced icing are clarified. At the defects, water molecules exhibit stronger adsorption and the heat transfer rate increases, accelerating the condensation of water vapor as well as ice nucleation and propagation. Moreover, the ice-defect interlocking structure increases the ice adhesion strength. Thus, a self-healing (at -20 °C) antifreeze-protein (AFP)-inspired anti-icing coating is developed. The coating is based on a design that mimics the ice-binding and non-ice-binding sites in AFPs. It enables the coating to markedly inhibit ice nucleation (nucleation temperature < -29.4 °C), prevent ice propagation (propagation rate < 0.00048 cm
/s), and reduce ice adhesion on the surface (adhesion strength < 38.9 kPa). More importantly, the coating can also autonomously self-heal at -20 °C, as a result of multiple dynamic bonds in its structure, to inhibit defect-induced icing processes. The healed coating sustains high anti-icing and deicing performance even under various extreme conditions. This work reveals the in-depth mechanism of defect-induced ice formation as well as adhesion, and proposes a self-healing anti-icing coating for outdoor infrastructures.
The tunable photoluminescence (PL) property is very important for gallium nitride (GaN) nanoparticles in the application of ultraviolet and blue optoelectronic devices, while conventional methods are ...not so satisfactory that alternative methods for preparing GaN nanoparticles should be studied. In this paper, ultra-small and well dispersed GaN nanoparticles are fabricated through femtosecond pulse laser ablation in air, water and ethanol. For the PL spectra of GaN nanoparticles, there are no shifts in air, red shifts in water and blue shifts in ethanol compared with the intrinsic PL spectra of bulk GaN. The X-ray photoelectron spectroscopy (XPS) results demonstrate that the various PL spectra can be due to the different components inside the GaN nanoparticles, which not only have effect on the PL emissions, but also greatly influence the intensity of PL. This study validates that the ablation environment has a great adjustable effect on the properties of GaN nanoparticles.
Id helix-loop-helix (HLH) proteins (Id1–4) bind E protein bHLH transcription factors, preventing them from forming active transcription complexes that drive changes in cell states. Id proteins are ...primarily expressed during development to inhibit differentiation, but they become re-expressed in adult tissues in diseases of the vasculature and cancer. We show that the genetic loss of Id1/Id3 reduces ocular neovascularization in mouse models of wet age-related macular degeneration (AMD) and retinopathy of prematurity (ROP). An in silico screen identifies AGX51, a small-molecule Id antagonist. AGX51 inhibits the Id1-E47 interaction, leading to ubiquitin-mediated degradation of Ids, cell growth arrest, and reduced viability. AGX51 is well-tolerated in mice and phenocopies the genetic loss of Id expression in AMD and ROP models by inhibiting retinal neovascularization. Thus, AGX51 is a first-in-class compound that antagonizes an interaction formerly considered undruggable and that may have utility in the management of multiple diseases.
Display omitted
•Genetic knockdown of Id proteins inhibits ocular neovascularization in mouse models•In silico screen and in vitro assays identified AGX51, an anti-Id small molecule•AGX51 treatment lead to pan-Id degradation, cell cycle arrest, and cell death•AGX51 phenocopies genetic Id loss, inhibiting pathologic ocular neovascularization
Wojnarowicz et al., describe the identification, by an in silico screen, and characterization of a small molecule, AGX51, that targets Id proteins. AGX51 treatment of cells lead to Id protein degradation, cell cycle arrest, and reduced cell viability. AGX51 inhibited pathologic ocular neovascularization in mouse models, phenocopying genetic Id loss.
PDF
