Protein regulator of cytokinesis 1 (PRC1) has been reported in correlation with various malignancies. Functionality of PRC1 in nasopharyngeal carcinoma (NPC) was investigated, in perspective of long ...noncoding RNA (lncRNA) regulatory circuitry. Aberrant expressed messenger RNA and lncRNA were screened out from the Gene Expression Omnibus microarray database. NPC cell line CNE‐2 was adopted for in vitro study and transfected with mimic or short hairpin RNA of miR‐194‐3p and PTPRG‐AS1. The radioactive sensitivity, cell viability, migration, invasion, and apoptosis were detected. PTPRG‐AS1 and PRC1 were upregulated in NPC, whereas miR‐194‐3p was downregulated. PTPRG‐AS1 was found to specifically bind to miR‐194‐3p as a competing endogenous RNA and miR‐194‐3p targets and negatively regulates PRC1. Overexpressed miR‐194‐3p or silenced PTPRG‐AS1 resulted in enhanced sensitivity to radiotherapy and cell apoptosis along with suppressed cell migration, invasion and proliferation in NPC. Furthermore, impaired tumor formation was also caused by miR‐194‐3p overexpression or PTPRG‐AS1 suppression through xenograft tumor in nude mice. In our study, PTPRG‐AS1/miR‐194‐3p/PRC1 regulatory circuitry was revealed in NPC, the mechanism of which can be of clinical significance for treatment of NPC.
In our study, PTPRG‐AS1/miR‐194‐3p/PRC1 regulatory circuitry was revealed in NPC, the mechanism of which can be of clinical significance for the treatment of NPC.
The cubature Kalman filter (CKF), which is based on the third degree spherical–radial cubature rule, is numerically more stable than the unscented Kalman filter (UKF) but less accurate than the ...Gauss–Hermite quadrature filter (GHQF). To improve the performance of the CKF, a new class of CKFs with arbitrary degrees of accuracy in computing the spherical and radial integrals is proposed. The third-degree CKF is a special case of the class. The high-degree CKFs of the class can achieve the accuracy and stability performances close to those of the GHQF but at lower computational cost. A numerical integration problem and a target tracking problem are utilized to demonstrate the necessity of using the high-degree cubature rules to improve the performance. The target tracking simulation shows that the fifth-degree CKF can achieve higher accuracy than the extended Kalman filter, the UKF, the third-degree CKF, and the particle filter, and is computationally much more efficient than the GHQF.
In this paper, a novel nonlinear filter named Sparse-grid Quadrature Filter (SGQF) is proposed. The filter utilizes weighted sparse-grid quadrature points to approximate the multi-dimensional ...integrals in the nonlinear Bayesian estimation algorithm. The locations and weights of the univariate quadrature points with a range of accuracy levels are determined by the moment matching method. Then the univariate quadrature point sets are extended to form a multi-dimensional grid using the sparse-grid theory. Compared with the conventional point-based methods, the estimation accuracy level of the SGQF can be flexibly controlled and the number of sparse-grid quadrature points for the SGQF is a polynomial of the dimension of the system, which alleviates the curse of dimensionality for high dimensional problems. The Unscented Kalman Filter (UKF) is proven to be a subset of the SGQF at the level-2 accuracy. The performance of this filter is demonstrated by an orbit estimation problem. The simulation results show that the SGQF achieves higher accuracy than the Extended Kalman Filter (EKF), the UKF, and the Cubature Kalman Filter (CKF). In addition, the SGQF is computationally much more efficient than the multi-dimensional Gauss–Hermite Quadrature Filter (GHQF) with the same performance.
The tumor-related myeloid derived suppressor cells (MDSCs), important immunosuppressive cells in tumor microenvironment, play an important role in the cancer progression. This study is aimed to ...investigate the crosstalk between MDSCs and oral squamous cell carcinoma (OSCC) cells and their role in the malignant progression of OSCC.
Immunochemistry (IHC) was used to investigate the expression of CD33 in 200 OSCC, 36 premalignant. CD33+ MDSCs were sorted and enriched via magnetic-activated cell sorting (MACS) from OSCC patients or health donor, and their phenotypes were identified by flow cytometry. With a co-culture system of MDSCs and OSCC, the effects of MDSCs on OSCC proliferation, apoptosis, migration invasion, epithelial-mesenchymal transition (EMT), and vasculogenic mimicry formation (VM) formation were assessed, respectively. Besides, peripheral blood mononuclear cells (PBMCs) from health donor were cultured with OSCC supernatant, the level of MDSCs and expressions of Arginase (Arg-1) and inducible nitric oxide synthase (iNOS) were measured.
The number of MDSCs was increased in tumor tissues of OSCC patients, and was positively related to the T stage, pathological grade, lymph node metastasis and poor prognosis. Tumor-related MDSCs of the co-culture system promoted OSCC progression by contributing to cell proliferation, migration and invasion as well as inducing EMT and VM. In turn, OSCC cells had potential to induce MDSCs differentiation from PBMCs and increase the expression of Arg-1 and iNOS.
These indicated that the crosstalk between MDSCs and tumor cells facilitated the malignant progression of OSCC cells and the immune suppressive properties of MDSCs, which may provide new insights into tumor treatment on targeting tumor-associated immunosuppressive cells.
•Heterostructured p-CuO/n-SnO2 core-shell NWs with precisely controlled shell thickness were synthesized by ALD technique.•The p-CuO/n-SnO2 core-shell NW structured gas sensors with 24 nm SnO2 shell ...layer exhibited enhanced sensing performance.•The well-structured core-shell NWs also have excellent selectivity for HCHO.
Highly sensitive and selective gas sensors based on heterostructured p-CuO/n-SnO2 core-shell nanowires (NWs) with precisely controlled shell thickness were synthesized through a sequential process combining a solution processing and atomic layer deposition. The gas sensing devices were fabricated on micro-electromechanical systems, which has triggered great research interest for low power consumption and highly integrated design. The designed p-CuO/n-SnO2 core-shell NW structured gas sensors exhibited superior gas sensing performance, which is closely related to the thickness of the SnO2 shell. Specifically, p-CuO/n-SnO2 core-shell NWs with a 24 nm thick SnO2 shell displayed a high sensitivity (Ra/Rg) of 2.42, whose rate of resistance change (i.e. 1.42) is 3 times higher than the pristine CuO NW sensor when detecting 50 ppm formaldehyde (HCHO) at 250 °C. The enhanced gas sensing performance could be attributed to the formation of p-n heterojunction which was revealed by specific band alignment and the heterojunction-depletion model. Besides, the well-structured p-CuO/n-SnO2 core-shell NWs achieved excellent selectivity for HCHO from commonly occurred reducing gases. In a word, such heterostructured p-CuO/n-SnO2 core-shell NW gas sensors demonstrate a feasible approach for enhanced sensitive and selective HCHO detection.
Two rare 5/5/5/6 four–ring system iridoids, allamancins A and B (1 and 2) together with one known biogenetically related iridoid derivative, 3‐O‐methyallamancin (3) were isolated from the flowers of ...Plumeria alba L. The structures of these iridoid derivatives were determined by comprehensive spectroscopic analyses. The absolute configuration of 1 was confirmed by X‐ray crystallographic analysis. The inhibitory activities of compounds 1–3 against nitric oxide (NO) production induced and three cancer cell lines were evaluated in vitro. Compounds 1 and 3 showed inhibitory activities on NO production with IC50 values of 18.3±0.12 and 22.1±0.14 μM, respectively. Compounds 1–3 showed moderate inhibitory activities against cancer cell lines of A549, Hela and MCF‐7.
A promising anode material consisting of bimetallic thiophosphate ZnxCo1−xPS3 and CoS2 with 2D/3D heterostructure is designed and prepared by an effective chemical transformation. Density functional ...theory calculations illustrate that the Zn2+ can effectively modulate the electrical ordering of ZnxCo1−xPS3 on the nanoscale: the reduced charge distribution emerging around the Zn ions can enhance the local built‐in electric field, which will accelerate the ions migration rate by Coulomb forces and provide tempting opportunities for manipulating Li+ storage behavior. Moreover, the merits of the large planar size enable ZnxCo1–xPS3 to provide abundant anchoring sites for metallic CoS2 nanocubes, generating a 2D/3D heterostructure with a strong electric field. The resultant ZnxCo1−xPS3/CoS2 can offer the combined advantages of bimetallic alloying and heterostructure in lithium storage applications, leading to outstanding performance as an anode material for lithium‐ion batteries. Consequently, a high capacity of 794 mA h g−1 can be retained after 100 cycles at 0.2 A g−1. Even at 3.0 A g−1, a satisfactory capacity of 465 mA h g−1 can be delivered. The appealing alloying‐heterostructure and electrochemical performance of this bimetallic thiophosphate demonstrate its great promise for applications in practical rechargeable batteries.
A promising anode material consisting of bimetallic thiophosphate ZnxCo1−xPS3 and CoS2 with 2D/3D heterostructure is prepared. The hetero‐Zn alloying can produce an enhanced asymmetric E‐field to accelerate electron transfer and adjust the interlayer distance to create small volume changed MPS3 electrodes. Additionally, metallic CoS2 deposited on semiconductor ZnxCo1−xPS3 can form a strong E‐field, favoring the transportation of electrons.
The present study evaluated the effects of dietary
Allium mongolicum Regel
polysaccharide (AMRP) on growth, lipopolysaccharide-induced antioxidant responses and immune responses in
Channa argus
. A ...basal diet was supplemented with AMRP at 0, 1, 1.5 or 2 g/kg feed for 56 days. After the 56 days feeding period, weight gain (WG), specific growth rate (SGR) and feed conversion ratio (FCR) were significantly increased or decreased (
P
< 0.05) by dietary AMRP, with the highest WG, SGR and the minimum FCR occurring in 1.5 g/kg AMRP group. Furthermore, AMRP supplementation conferred significant protective effects against LPS challenge by preventing alterations in the levels of complements 3 (C3) and complements 4 (C4), lysozyme, superoxide dismutase (SOD), glutathione-S-transferase (GST), interleukin-1β (IL-1β) and tumour necrosis factor-α (TNF-α) while regulating the expression of immune-related genes including heat shock protein 70 (HSP70), heat shock protein 90 (HSP90), SOD, GST, IL-1 and TNF-α. Finally, AMRP supplementation significantly increased serum total protein, albumin and globulin concentrations and reduced mortality after LPS challenge. Taken together, our results suggest that the administration of AMRP could attenuate LPS-induced negative effects in
C. argus
, with 1.5 g/kg considered a suitable dose.
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