Ultrasounds (US) are one of the most used imaging techniques in medicine for assessing the physiological and pathological state of soft tissue. Apart from therapeutic applications, most of the ...interaction of the acoustic beams with tissues occur passively and without substantial modification to the physiology of the latter. However, US can also be used to remotely power implantable devices with sensing capabilities. In this study, we propose small-form devices interfaced with functionalized electrochemical electrodes for the detection of pH and lactate levels, powered by ultrasounds and data transmission through a Frequency Shift Keying (FSK) modulation technique. A custom-made piezoelectric transducer is responsible for converting the acoustic waves into electrical voltage at the device with operational levels as low as 0.5 V (power consumption of 10 μW) obtained from implantation distances of 50 mm inside tissue. This conjugated with the high sensitivity of the developed electrochemical sensors allows to detect and transmit local parameter variations below 0.1 pH (4.2 mV) and 1 mM lactate (70 nA). Potential applications include real-time access to intrabody tissue monitoring post-operatively, with the view of assessing proper soft tissue healing or infection detection by bacteria, as well as tissue cancer screening in structures such as the human breast.
•Fully implantable device powered by ultrasounds with data transmission on the backscattered acoustic echoes.•Interface to functionalized electrodes for detection of pH and lactate levels in soft tissue.•Sensitivities below 0.1 pH (4.2 mV potential) and 1 mM lactate (70 nA current) levels.•Device operation up to distances of 50 mm (10 μW) as tested inside an anatomical model of the human breast.•Potential application in soft tissue monitoring post-operatively for healing assessment or infection detection.
Formal safety assessment (FSA), as a structured and systematic risk evaluation methodology, has been increasingly and broadly used in the shipping industry around the world. Concerns have been raised ...as to navigational safety of the Yangtze River, China's largest and the world's busiest inland waterway. Over the last few decades, the throughput of ships in the Yangtze River has increased rapidly due to the national development of the Middle and Western parts of China. Accidents such as collisions, groundings, contacts, oil-spills and fires occur repeatedly, often causing serious consequences. In order to improve the navigational safety in the Yangtze River, this paper estimates the navigational risk of the Yangtze River using the FSA concept and a Bayesian network (BN) technique. The navigational risk model is established by considering both probability and consequences of accidents with respect to a risk matrix method, followed by a scenario analysis to demonstrate the application of the proposed model.
Quercetin (3,3′,4′,5,7-pentahydroxyflavone, Qu) is a promising cancer chemo-preventive agent for various cancers because it inhibits disease progression and promotes apoptotic cell death. In our ...previous study, we demonstrated that Qu could evoke ER stress to enhance drug cytotoxicity in ovarian cancer (OC). However, Qu-induced ER stress in OC is still poorly understood. Here, we demonstrated that Qu evoked ER stress to involve in mitochondria apoptosis pathway via the p-STAT3/Bcl-2 axis in OC cell lines and in primary OC cells. Unexpectedly, inhibition of ER stress did not reverse Qu-induced cell death. Further functional studies revealed that Qu-induced ER stress could activate protective autophagy concomitantly by activating the p-STAT3/Bcl-2 axis in this process. Moreover, the autophagy scavenger 3-MA was shown to enhance Qu’s anticancer effects in an ovarian cancer mice xenograft model. These findings revealed a novel role of ER stress as a “double edge sword” participating in Qu-induced apoptosis of OC and might provide a new angle to consider in clinical studies of biological modifiers that may circumvent drug resistance in patients by targeting protective autophagy pathways.
A unified hypoplastic model is formulated by incorporating the anisotropic critical state theory to describe the fabric effect in sand under both monotonic and cyclic loading conditions. An evolving ...deviatoric fabric tensor that characterizes the internal microstructure of sand is introduced into the hypoplastic model in conjunction with the intergranular strain concept. A scalar-valued fabric anisotropic variable indicating the interplay between the fabric and the loading direction is employed to account for the impact of fabric anisotropy on both the dilatancy and shear strength of sand. The model is demonstrated to be capable of simulating the anisotropic behavior of sand, using a single set of parameters under both monotonic and cyclic loading conditions, as evidenced by the satisfactory match with experimental results from various sources. In particular, by considering the influence of fabric evolution on the dilatancy of sand, the model adequately accounts for the fabric change effect and accurately captures the deviatoric strain accumulation, cyclic mobility, and the flow liquefaction phenomenon under cyclic loading condition.
Despite much recent effort, the highest reported T c of the infinite-layer nickelates remains lower than 15 K. Here, the authors apply pressure to Pr0.82Sr0.18NiO2 thin films and observe a monotonic ...increase of T c to 31 K at 12.1 GPa, an increase that does not level off with increasing pressure.
Ecdysteroids, as the key growth hormones, regulate moulting, metamorphosis and reproduction in arthropods. Ecdysteroid biosynthesis is catalysed by a series of cytochrome P450 monooxygenases ...(CYP450s) encoded by Halloween genes, including spook (spo), phantom (phm), disembodied (dib), shadow (sad) and shade (shd). The ecdysteroid biosynthesis in insects is clear with 20‐hydroxyecdysone (20E) as the main ecdysteroid. However, the information on the major ecdysteroids in arachnids is limited. In this study, Halloween genes spo, dib, sad and shd, but not phm, were identified in the pond wolf spider, Pardosa pseudoannulata. Phylogenetic analysis grouped arachnid and insect Halloween gene products into two CYP450 clades, the CYP2 clan (spo and phm) and the mitochondrial clan (dib, sad, and shd). In P. pseudoannulata, the temporal expression profile of the four Halloween genes in concurrence with spiderling moulting with steady increase in the course of the 2nd instar followed by a rapid dropdown once moulting was completed. Spatially, the four Halloween genes were highly expressed in spiderling abdomen and in the ovaries of female adults. In parallel, ponasterone A (PA), but not 20E, was detected by LC–MS/MS analysis in P. pseudoannulata, and it was demonstrated as a functional ecdysteroid in the spider by accelerating of moulting with PA addition. The present study revealed the different ecdysteroid biosynthesis pathways in spiders and insects.
Four Halloween genes were identified in the spider Pardosa pseudoannulata.
The four genes have an increasing temporal expression within a moulting cycle and a specific spatial expression in abdomen and ovary.
Ponasterone A was detected and demonstrated as a functional ecdysteroid in the spider.
Recovering a sparse signal from an undersampled set of random linear measurements is the main problem of interest in compressed sensing. In this paper, we consider the case where both the signal and ...the measurements are complex-valued. We study the popular recovery method of l 1 -regularized least squares or LASSO. While several studies have shown that LASSO provides desirable solutions under certain conditions, the precise asymptotic performance of this algorithm in the complex setting is not yet known. In this paper, we extend the approximate message passing (AMP) algorithm to solve the complex-valued LASSO problem and obtain the complex approximate message passing algorithm (CAMP). We then generalize the state evolution framework recently introduced for the analysis of AMP to the complex setting. Using the state evolution, we derive accurate formulas for the phase transition and noise sensitivity of both LASSO and CAMP. Our theoretical results are concerned with the case of i.i.d. Gaussian sensing matrices. Simulations confirm that our results hold for a larger class of random matrices.
The objective of this experiment was to investigate the effects of betaine on growth performance, serum parameters, intestinal health, and immune performance of goslings in response to ...lipopolysaccharide (LPS) challenge. A total of 168 healthy male 15-day-old Jiangnan White Goslings were randomly divided into 4 groups, with 6 replicates per treatment and seven goslings per replicate. A 2 × 2 factorial arrangement included 2 factors, that is, LPS challenge (injection of LPS or physiological saline) and betaine (added 0 or 0.06% betaine in diet). The results indicated that LPS challenge significantly reduced the average daily feed intake (ADFI), average daily gain (ADG), and body weight (BW) at 21 D of the goslings, while dietary betaine supplementation tended to increase the ADFI during the LPS stress period (P = 0.08) and BW at 21 D of the goslings (P = 0.09). The LPS-challenged goslings showed higher pro-inflammatory cytokines (interleukin-1 IL-1β, interleukin-6 IL-6, tumor necrosis factor-α (TNF-α), and Interferon-gamma IFN-γ) and lower anti-inflammatory cytokine (Interleukin-10 IL-10) (P < 0.05) at 21 D of age. Dietary betaine supplementation alleviated LPS-induced increase in pro-inflammatory cytokines. The LPS challenge significantly decreased duodenal and jejunal villus height (VH) and villus height and crypt depth ratio (VCR), while the addition of betaine significantly increased duodenal VH and VCR (P < 0.05). On the other hand, addition of betaine significantly alleviated decline of enzyme activity on lipase, amylase, trypsin, and chymotrypsin in the intestinal of goslings. The LPS challenge significantly increased the content of serum D-lactic acid (D-LA) and the activity of diamine oxidase (DAO) at 21 D of the goslings. The LPS challenge and betaine addition significantly increased the mRNA expression of Occcludin (OCLN) in jejunal mucosa at 28 D of the goslings (P < 0.05). In conclusion, our research demonstrated that betaine can alleviate the decline of growth performance and immune performance in goslings caused by LPS. The results also indicate betaine possesses anti-inflammation properties and improves intestinal barrier functions. We recommend that 0.06% betaine be added into the diet to improve the intestinal health and immune performance of goslings.
•Status of implementation of wastewater treatment for reuse as potable water reviewed.•>50% of all schemes use RO followed by UV disinfection, a few with UV AOP final step.•QMRA studies show risk ...imposed by controlled reuse smaller than from de facto reuse.•Implementation becomes challenging if unbounded environmental waters used as buffers.•Pivotal requirement is reliable, on-line monitoring of pathogens and key CECs.
A review of the current status of direct and indirect potable water reuse (DPR/IPR) implementation has been conducted, focusing on the regulatory and practical aspects and with reference to the most recent published literature. The review encompasses (a) the principal contaminant types, their required removal and the methods by which their concentration is monitored, (b) regulatory approaches and stipulations in assessing/ratifying treatment schemes and maintaining treated water quality, and (c) existing full-scale installations. Analytical methods discussed include established in-line monitoring tools, such as turbidity measurement, to more recent polymerase chain reaction (PCR)-based assay methods for microbial detection. The key risk assessment tools of quantitative microbial risk assessment (QMRA) and water safety plans (WSPs) are considered in relation to their use in selecting/ratifying treatment schemes, and the components of the treatment schemes from 40 existing IPR/DPR installations summarised. Five specific schemes are considered in more detail.
The review reveals:1over half of the schemes identified employ reverse osmosis (RO) followed by UV disinfection, with UV-based advanced oxidation used in many modern schemes as the final step;2Whilst quantitative PCR appears to offer many advantages for microbial detection, due to its sensitivity and specificity, it nonetheless demands pre-concentration of the sample and is subject to interference leading to possible false positives;3QMRA studies suggest that the risk imposed by DPR and, in particular, IPR is very small compared with de facto reuse, the latter being subject to far less regulatory scrutiny;4There appears to be no evidence of acute conditions, and diarrhoeal disease specifically, from the few epidemiological studies which have been conducted; and.5IPR implementation becomes challenging if unbounded environmental waters are used as a buffer, since “zero deterioration” in environmental quality must then be demonstrated.Whilst there are a number of ongoing projects where RO is not used because of the challenge imposed by disposal of RO concentrate, the prevalence of the sequential RO-UV combination implies the importance of quantifying the impact of process upsets on these unit operations.
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