Background and objectives
We studied the effects of different cultivars of non‐degermed millet flour fraction and the particle size on Chinese steamed bread to promote the industrial development of ...the whole‐grain food industry.
Findings
We used a texture analyzer to analyze the changes introduced by different cultivars of non‐degermed millet flour fraction, and the effect of its particle size on the hardness, adhesiveness, springiness, gumminess, and chewiness parameters, based on the analysis of height‐to‐diameter ratio and specific volume. We obtained samples of different particle size fractions by using 40, 60, 80, 100, 120, and 140 mesh sieves and determined that the particle substitution of non‐degermed millet flour fraction was at a level of 30%. We conducted partial least squares discriminant analysis (PLS‐DA) screening to identify the signature differences of quality components.
Conclusions
According to Hunter Laboratory values (whiteness/darkness, L*; redness/greenness, a*; and yellowness/blueness, b*), the results indicated that as the particle size decreased, the L* and whiteness index (W) values decreased but the a* and b* values increased, and the hardness, cohesiveness, adhesiveness, springiness, gumminess, and chewiness parameters of Chinese steamed bread with different particle sizes were significantly different (p < .05).
Significance and novelty
Partial least squares discriminant analysis could distinguish japonica cultivar samples and glutinous cultivar samples. According to the variable influence on projection (VIP) values, we screened chewiness, gumminess, and hardness as the signature differences of the quality components.
We study the ground state phases of quasi-one-dimensional spin-orbit coupled spin-1 Bose-Einstein condensates trapped in harmonic potential. Based on variational analysis, the conditions of phase ...transition between magnetized and unmagnetized phases are obtained analytically for the first time, and the physical mechanism of inducing phase transition is revealed. The competition relationship among spin-independent interaction, spin-dependent interaction, spin-orbit (SO) coupling and harmonic potential for generating phase transition is discussed systematically. We predict that the spin-dependent interaction plays key role in ground state phase transition. Interestingly, the phase transition of the ground state degenerate to the single particle case in free space when spin-dependent interaction is absent. In addition, the harmonic potential strengthens the influence of atomic interactions on the phase boundary. Our results provide theoretical evidence for deep understanding the ground sate phase transition of the system.
•Magnetized and non-magnetized phases of trapped SO coupled spin-1 BECs are studied.•The phase transition condition is obtained analytically for the first time.•The physical mechanism of inducing phase transition is revealed explicitly.•The spin-dependent interaction and trapping potential play key role in phase transition.•We provide theoretical evidence for manipulating the ground sate of the system.
Ladder climbing (LC) and autoresonance (AR) of the spherical plasma density wave are studied for the first time. The governing equation of the perturbed spherical density wave in the energy level ...space based on a hydrodynamic model of the electron plasma is presented, and it is demonstrated that the quantum LC and classical AR transition can be achieved in the spherical plasma. The asymptotic thresholds of the LC and AR transition of the spherical plasma wave are obtained analytically and confirmed numerically. We find that the spherical wave energy is concentrated to the sphere center as the density wave climbs to the higher level, the spherical plasma behaves obvious compression character, and the perturbed density of the sphere center even can be amplified to 100 times larger of the initial perturbed density. Compared to the one-dimensional case, the energy spectrum of the spherical plasma wave shifts upward, and the energy level spacing of the spherical plasma wave is broadened. These result in the facts that the spherical plasma needs the larger driving strength to achieve the LC and AR, while the total perturbed density of the spherical plasma always is larger than that of the one-dimensional case.
The realization and detection of two-leg ladders with the interplay of the artificial gauge field provide an ideal platform for investigations of the quantum phase transition and quantum dynamics. ...Here, we realize the spatio-temporal evolution dynamics of two-leg ladder systems with magnetic flux analytically and numerically. The quench dynamics induced by giving sudden quenches of initial density distributions of left and right leg components, rung-to-leg coupling ratios and magnetic flux from the ground state are discussed in detail. Affected by the chirality of ladder systems together with the tunneling between the two legs, rich and interesting quench dynamics observed, which including the Josephson Oscillations (JOs), Chiral Density Wave (CDW), Scissors Mode (SM), Periodic Torsional Vortex (PTV) and Periodic Torsional-Scissors Vortex (PTSV). Remarkably, we find that the oscillation frequency of the quench dynamics associated with coupling ratios and magnetic flux can be used to identify the phase transition between the Meissner phase and Vortex phase. Therefore, our work may provide an effective way to distinguish phase transitions by using quench dynamics.
The enzyme flavone synthase Is (FNS Is) converts flavanones to flavones, whereas flavanone 3β-hydroxylases (F3Hs) catalyze the formation of dihydroflavonols, a precursor of flavonols and ...anthocyanins. Canonical F3Hs have been characterized in seed plants, which are evolutionarily related to liverwort FNS Is. However, as important evolutionary lineages between liverworts and seed plants, ferns FNS Is and F3Hs have not been identified. In the present study, we characterized a bifunctional enzyme PnFNS I/F3H from the fern Psilotum nudum. We found that PnFNS I/F3H catalyzed the conversion of naringenin to apigenin and dihydrokaempferol. In addition, it catalyzed five different flavanones to generate the corresponding flavones. Site-directed mutagenesis results indicated that the P228-Y228 mutant protein displayed the FNS I/F2H activity (catalyzing naringenin to generate apigenin and 2-hydroxynaringenin), thus having similar functions as liverwort FNS I/F2H. Moreover, the overexpression of PnFNS I/F3H in Arabidopsis tt6 and dmr6 mutants increased the content of flavones and flavonols in plants, further indicating that PnFNS I/F3H showed FNS I and F3H activities in planta. This is the first study to characterize a bifunctional enzyme FNS I/F3H in ferns. The functional transition from FNS I/F3H to FNS I/F2H will be helpful in further elucidating the relationship between angiosperm F3Hs and liverwort FNS Is.
•It is the first report of conducting activity analyses for ferns FNS I/F3H.•The PnFNS I/F3H-P228Y mutant activity was the same as FNS I/F2H from liverworts.•PnFNS I/F3H was probably responsible for the biosynthesis of both flavones and flavonols in planta.
The efficient simultaneous activation of cyclohexenones or simple alkyl acyclic enones and 2‐hydroxynitrostyrenes was realized by using a prolinol thioether catalyst. Thus, a highly chemo‐, ...diastereo‐ and enantioselective synthesis of functionalized tetrahydroxanthenones and chromanes with up to three contiguous stereocenters was developed.
Acetolactate synthase (ALS)-inhibiting herbicide tribenuron-methyl (TBM) is an efficient gametocide that can cause rapeseed (Brassica napus L.) to become male sterile and outcrossing. To find the ...reason the TBM treatment leads to male sterility, an integrated study using cytological, physiological, and transcriptomic methods was conducted.
Some temporary symptoms, including the discoloration of young leaves and a short halt of raceme elongation, were observed in the rapeseed plants exposed to TBM at an application rate of 1 μg per plant. Both chloroplasts in young leaves and plastids in anthers were deformed. TBM also reduced the leaf photosynthetic rate and the contents of chlorophyll, soluble sugar and pyruvate. Both the tapetal cells and uni-nucleate microspores in the treated plants showed large autophagic vacuoles, and the tissue degenerated quickly. A transcriptomic comparison with the control identified 200 upregulated and 163 downregulated differential expression genes in the small flower buds of the TBM treatment. The genes encoding functionally important proteins, including glucan endo-1,3-beta-glucosidase A6, QUARTET3 (QRT3), ARABIDOPSIS ANTHER 7 (ATA7), non-specific lipid-transfer protein LTP11 and LTP12, histone-lysine N-methyltransferase ATXR6, spermidine coumaroyl-CoA acyltransferase (SCT), and photosystem II reaction centre protein psbB, were downregulated by TBM exposure. Some important genes encoding autophagy-related protein ATG8a and metabolic detoxification related proteins, including DTX1, DTX6, DTX35, cytosolic sulfotransferase SOT12, and six members of glutathione S-transferase, were upregulated. In addition, several genes related to hormone stimulus, such as 1-aminocyclopropane-1-carboxylate synthase 8 (ACS8), ethylene-responsive factor ERF1A, ERF1, ERF71, CRF6, and RAP2-3, were also upregulated. The transcriptional regulation is in accordance with the functional abnormalities of pollen wall formation, lipid metabolism, chloroplast structure, ethylene generation, cell cycle, and tissue autophagy.
The results suggested that except for ALS, the metabolic pathways related to lipid metabolism, pollen exine formation, photosynthesis and hormone response are associated with male sterility induced by TBM. The results provide new insight into the molecular mechanisms of inducing male sterility by sulfonylurea.
Isoflavones are rich natural compounds present in legumes and are essential for plant growth and development. Moreover, they are beneficial for animals and humans. Isoflavones are primarily found as ...glycoconjugates, including calycosin-7-O-β-d-glucoside (CG) in Astragalus membranaceus, a legume. However, the glycosylation mechanism of isoflavones in A. membranaceus remains unclear. In the present study, three uridine diphosphate (UDP)-glycosyltransferases (UGTs) that may be involved in the biosynthesis of isoflavone were identified in the transcriptome of A. membranaceus. Enzymatic analysis revealed that AmUGT88E29 and AmUGT88E30 had high catalytic activity toward isoflavones in vitro. In addition, AmUGT88E29 and AmUGT88E30 could accept various flavones, flavanones, flavonols, dihydroflavonols, and dihydrochalcones as substrates. AmUGT71G10 was only active against phloretin and dihydroresveratrol. Overexpression of AmUGT88E29 significantly increased the contents of CG, an isoflavone glucoside, in the hairy roots of A. membranaceus. This study provided candidate AmUGT genes for the potential metabolic engineering of flavonoid compounds in plants and a valuable resource for studying the calycosin glycosides biosynthesis pathway.
A highly efficient organocatalytic Michael addition/I2‐mediated cyclization reaction sequence between 1,3‐diketones and unsaturated pyrazolones was achieved in the presence of a chiral ...cinchonidine‐derived squaramide catalyst and I2 under mild reaction conditions. A variety of optically active spiro‐dihydrofuran pyrazolones were obtained in good to high yields (71–97 %) with excellent diastereoselectivities and high to excellent enantioselectivities (up to >99:1 dr and 99 % ee). A gram‐scale synthesis of chiral spiro‐dihydrofuran pyrazolones was also successfully realized.
A highly efficient organocatalytic Michael addition/I2‐mediated cyclization reaction sequence between 1,3‐diketones and unsaturated pyrazolones was achieved. A variety of optically active spiro‐dihydrofuran pyrazolones were obtained by using this method. The gram‐scale synthesis of chiral spiro‐dihydrofuran pyrazolones was also successfully realized.
The biosynthesis of flavonoids and anthocyanidins has been exclusively investigated in angiosperms but largely unknown in ferns. This study integrated metabolomics and transcriptome to analyze the ...fronds from different development stages (S1 without spores and S2 with brown spores) of
Cyclosorus parasiticus
. About 221 flavonoid and anthocyanin metabolites were identified between S1 and S2. Transcriptome analysis revealed several genes encoding the key enzymes involved in the biosynthesis of flavonoids, and anthocyanins were upregulated in S2, which were validated by qRT-PCR. Functional characterization of two chalcone synthases (CpCHS1 and CpCHS2) indicated that CpCHS1 can catalyze the formation of pinocembrin, naringenin, and eriodictyol, respectively; however, CpCHS2 was inactive. The crystallization investigation of CpCHS1 indicated that it has a highly similar conformation and shares a similar general catalytic mechanism to other plants CHSs. And by site-directed mutagenesis, we found seven residues, especially Leu199 and Thr203 that are critical to the catalytic activity for CpCHS1.
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