Penaeid shrimp has a distinctive metamorphosis stage during early development. Although morphological and biochemical studies about this ontogeny have been developed for decades, researches on gene ...expression level are still scarce. In this study, we have investigated the transcriptomes of five continuous developmental stages in Pacific white shrimp (Litopenaeus vannamei) with high throughput Illumina sequencing technology. The reads were assembled and clustered into 66,815 unigenes, of which 32,398 have putative homologues in nr database, 14,981 have been classified into diverse functional categories by Gene Ontology (GO) annotation and 26,257 have been associated with 255 pathways by KEGG pathway mapping. Meanwhile, the differentially expressed genes (DEGs) between adjacent developmental stages were identified and gene expression patterns were clustered. By GO term enrichment analysis, KEGG pathway enrichment analysis and functional gene profiling, the physiological changes during shrimp metamorphosis could be better understood, especially histogenesis, diet transition, muscle development and exoskeleton reconstruction. In conclusion, this is the first study that characterized the integrated transcriptomic profiles during early development of penaeid shrimp, and these findings will serve as significant references for shrimp developmental biology and aquaculture research.
Green tides are marine disasters caused by the explosive proliferation or high concentration of certain large algae in seawater, which causes discoloration of the water body. Accurate monitoring of ...its distribution area is highly important for early warning and the protection of marine ecology. However, existing deep learning methods have difficulty in effectively identifying green tides with anisotropic characteristics due to the complex and variable shapes of the patches and the wide range of scales. To address this issue, this paper presents an anisotropic green tide patch extraction network (AGE-Net) based on deformable convolution. The main structure of AGE-Net consists of stacked anisotropic feature extraction (AFEB) modules. Each AFEB module contains two branches for extracting green tide patches. The first branch consists of multiple connected dense blocks. The second branch introduces a deformable convolution module and a depth residual module based on a multiresolution feature extraction network for extracting anisotropic features of green tide patches. Finally, an irregular green tide patch feature enhancement module is used to fuse the high-level semantic features extracted from the two branches. To verify the effectiveness of the AGE-Net model, experiments were conducted on the MODIS Green Tide dataset. The results show that AGE-Net has better recognition performance, with F1-scores and IoUs reaching 0.8317 and 71.19% on multi-view test images, outperforming other comparison methods.
Valnemulin (VML) is a semi-synthetic pleuromutilin derivative widely used to treat animal bacterial diseases. However, no study has comprehensively evaluated VML metabolism in aquatic animals, ...including crucian carp. This study aimed to investigate VML metabolism in crucian carp. VML metabolites in crucian carp were quantified via intraperitoneal injection and analyzed via ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Three VML metabolites were detected in crucian carp via ultra-performance liquid chromatography-tandem quadrupole and time-of-flight mass spectrometry (UPLC-Q-TOF/MS) structural analysis. The enrichment and metabolism rules of the metabolites were summarized based on tissue distribution and concentration changes of the three metabolites. The metabolites were mainly found in the liver at 0.1 h after VML injection. The levels of the metabolites were abundant in the bile from 4 h to 12 h and in the skin after 72 h. The levels of the metabolites in the bile first increased, then decreased. The metabolism in the liver was completed at 72 h. The metabolites were detected in the skin following a 72 h period, which increased with time.
In the field of mine blasting practice, accurate prediction of blasting vibration is considered as a critical task. In accordance with the theory of elastic wave, the reflection of blasting vibration ...wave at the interface of negative elevation is analyzed in this paper. The negative elevation effect exerted by blasting vibration is interpreted considering the mechanism, and the formula of the blasting vibration prediction step (negative) V=KQ1/3/Rα⋅1/Hβ is derived, reflecting the negative elevation effect. Besides, the formula accuracy is verified by the measured vibration velocity of the mass in the Jinou coal mine. The step (negative) formula acts as a more feasible candidate for the prediction of step blasting vibration.
Machine learning for causal inference, particularly at the individual level, has attracted intense interest in many domains. Existing techniques focus on controlling differences in distribution ...between treatment groups in a data-driven manner, eliminating the effects of confounding factors. However, few of the current methods adequately discuss the difference in treatment group sizes. Two approaches, a direct and an indirect one, deal with potential missing data for estimating individual treatment with binary treatments and different treatment group sizes. We embed the two methods into certain frameworks based on the domain adaption and representation. We validate the performance of our method by two benchmarks in the causal inference community: simulated data and real-world data. Experiment results verify that our methods perform well.
Two triple interpenetrating Zn(II)-based MOFs were studied in this paper. Named Zn6(1,4-bpeb)4(IPA)6(H2O)n (MOF-1) and {Zn3(1,4-bpeb)1.5(DDBA)3n·2DMF} (MOF-2), {1,4-bpeb = 1,4-bis 2-(4-pyridy1) ...ethenylbenze, IPA = Isophthalic acid, DDBA = 3,3′-Azodibenzoic acid}, they were synthesized by the hydrothermal method and were characterized and stability tested. The results showed that MOF-1 had good acid–base stability and solvent stability. Furthermore, MOF-1 had excellent green fluorescence and with different phenomena in different solvents, which was almost completely quenched in acetone. Based on this phenomenon, an acetone sensing test was carried out, where the detection limit of acetone was calculated to be 0.00365% (volume ratio). Excitingly, the MOF-1 could also be used as a proportional fluorescent probe to specifically detect tryptophan, with a calculated detection limit of 34.84 μM. Furthermore, the mechanism was explained through energy transfer and competitive absorption (fluorescence resonance energy transfer (FRET)) and internal filtration effect (IFE). For antibacterial purposes, the minimum inhibitory concentrations of MOF-1 against Escherichia coli and Staphylococcus aureus were 19.52 µg/mL and 39.06 µg/mL, respectively, and the minimum inhibitory concentrations of MOF-2 against Escherichia coli and Staphylococcus aureus were 68.36 µg/mL and 136.72 µg/mL, respectively.
Objective. This study aimed to systematically assess the effect of recombinant human epidermal growth factor (rhEGF) associated with conventional drugs on the score of dry eye symptoms in patients ...with dry eyes after cataract surgery. Methods. The online database was searched for the clinical controlled trials of rhEGF associated with conventional drugs in the therapy of dry eyes after cataract surgery. Until now, the retrieval timeframe is based on the establishment of the database. Separately, two researchers extracted the data. The bias risk of each included literature was assessed. Results. Eight clinical controlled studies were finally included, with 878 samples. The success rate of the study group was greatly higher, and the difference was statistically significant (P<0.05). The fluorescein staining (FL) score of the research group after treatment was lower, and the difference was statistically significant (P<0.05). Compared with the control group, the tear break up time (BUT) of the study group after treatment was notably prolonged. The dry eye symptom score of the research group after treatment was notably lower, and the difference was statistically significant (P<0.05). Conclusion. Sodium hyaluronate associated with rhEGF eye drops is successful to treat xerophthalmia after cataract operation. It successfully promotes corneal healing, promotes tear film stability, and increases basic tear secretion. This treatment scheme is worth popularizing and applying in clinics.
In this paper, we develop a novel method to construct Goethals–Seidel (GS) sequences with special structures. In the existing methods, utilizing Turyn sequences is an effective and convenient ...approach; however, this method cannot cover all GS sequences. Motivated by this, we are devoted to designing some sequences that can potentially construct all GS sequences. Firstly, it is proven that a quad of ±1 polynomials can be considered a linear combination of eight polynomials with coefficients uniquely belonging to {0,±1}. Based on this fact, we change the construction of a quad of Goethals–Seidel sequences to find eight sequences consisting of 0 and ±1. One more motivation is to obtain these sequences more efficiently. To this end, we make use of the k-block, of which some properties of (anti) symmetry are discussed. After this, we can then look for the sequences with the help of computers since the symmetry properties facilitate reducing the search range. Moreover, we find that one of the eight blocks, which we utilize to construct GS sequences directly, can also be combined with Williamson sequences to generate GS sequences with more order. Several examples are provided to verify the theoretical results. The main contribution of this work is in building a bridge linking the GS sequences and eight polynomials, and the paper also provides a novel insight through which to consider the existence of GS sequences.
White spot syndrome virus (WSSV) is a major pathogen in shrimp cultures. The interactions between viral proteins and their receptors on the surface of cells in a frontier target tissue are crucial ...for triggering an infection. In this study, a yeast two-hybrid (Y2H) library was constructed using cDNA obtained from the stomach and gut of Litopenaeus vannamei, to ascertain the role of envelope proteins in WSSV infection. For this purpose, VP37 was used as the bait in the Y2H library screening. Forty positive clones were detected after screening. The positive clones were analyzed and discriminated, and two clones belonging to the peritrophin family were subsequently confirmed as genuine positive clones. Sequence analysis revealed that both clones could be considered as the same gene, LV-peritrophin (LvPT). Co-immunoprecipitation confirmed the interaction between LvPT and VP37. Further studies in the Y2H system revealed that LvPT could also interact with other WSSV envelope proteins such as VP32, VP38A, VP39B, and VP41A. The distribution of LvPT in tissues revealed that LvPT was mainly expressed in the stomach than in other tissues. In addition, LvPT was found to be a secretory protein, and its chitin-binding ability was also confirmed.
Cu(2)O/MWCNT (multi-walled carbon nanotubes) nanocomposites were successfully prepared in large quantities with a new fixure-reduction method under low temperature. The morphology and shape of the ...Cu(2)O/MWCNTs nanocomposites were characterized by field emission scanning electron microscopes (FESEMs), energy dispersive X-ray (EDX), X-ray photoelectron spectroscopy (XPS) and X-ray powder diffraction (XRD), respectively. Cyclic voltammetry (CV) was used to evaluate the electrochemical performance of the Cu(2)O/MWCNTs nanocomposites modified electrode towards glucose. Compared to the bare GCE, the Cu(2)O nanoparticles and the MWCNTs modified electrode, the Cu(2)O/MWCNTs modified electrode displays high electrocatalytic activity towards the oxidation of glucose. With a potential of -0.20 V, the Cu(2)O/MWCNTs modified electrode was used to determine glucose by amperometric, showing significantly lower overvoltage and a linear dependence (R=0.9958) in the concentration up to 10 microM with a sensitivity of 6.53 microA micromol L(-1) and a detection limit of 0.05 micromol L(-1) (signal-to-noise ratio of 3). In summary, the preparation process of the nanocomposites is very simple and the nanocomposites could be used for the development of enzyme-free glucose sensor.
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