Background：Non-small cell lung cancer （NSCLC） is a prolific and high-mortality disease with few effective treatments.Although the detection and surgical techniques for NSCLC continue to advance,the ...survival rate for the patients with NSCLC remains poor.Enhanced predictive biomarkers such as microRNAs （miRNAs） are needed at the time of diagnosis to better tailor therapies for patients.This study focused on the expression ofmiR-1280 in NSCLC tissues and distal normal tissues in order to explore the association between miR-1280 expression and NSCLC.Methods：A total of 72 newly diagnosed primary NSCLC patients were enrolled in this study.Quantitative real-time polymerase chain reaction （PCR） was performed to identify the expression level ofmiR-1280 in the NSCLC tissues and distal normal tissues of these patients.Results：The miR-1280 expression was significantly higher in the NSCLC tissues （0.084 ± 0.099） than distal normal tissues （0.014 ± 0.015,P =0.009）.In 54 patients （75%）,the miR-1280 expression in the NSCLC tissues was upregulated （2-△△ct 〉 2）,and no case showed a downregulation of miR-1280 expression.Conclusions：The expression level ofmiR-1280 could be regarded as a biomarker for NSCLC.
WC-8Co hardmetals with different proportions of prismatic WC grains and plate-like WC grains were directly produced through sintering the W-C-8Co ele- mental powder mixture which was fabricated by ...dielectric barrier discharge plasma （DBDP）-assisted milling. The morphology of prepared WC-8Co hardmetals, geometry and the preferential orientation of plate-like WC were investigated by X-ray diffraction （XRD） and scanning electron microscopy （SEM） analysis. The results demon- strate that the microstructure and mechanical properties of the sintered hardmetals are related to the morphology of W grain which is dependent on DBDP-milling time. The DBDP for 1 h （DBDP-1 h）-milled W-C-Co powder contains granular W particles that tend to form prismatic WC grains, while the DBDP for 3 h （DBDP-3 h）-milled powder contains lamellar W particles that generate plate-like WC grains. By adjusting the weight ratio of DBDP-1 h powder and DBDP-3 h powder in W-C-8Co mixture, the proportion of plate-like WC in the hardmetals can be controlled, and relatively high transverse rupture strength （TRS） is obtained as the proportion of plate-like WC grain in the hardmetals is about 35 % in present experimental condition.
Aim： To find new kinase inhibitors that overcome time imatinib resistance in treatment of chronic myeloid leukemia （CML）, we synthesized C817, a novel derivative of curcumin, and tested its ...activities against wild-type （WT） and imatinib-resistant mutant Abl kinases, as well as in imatinib-sensitive and resistant CML cells in vitro. Methods： 32D cells harboring WT or mutant Abl kinases （nucleotide binding P-loop mutants Q252H, Y253F, and imatinib contact residue mutant T3151）, as well as K562/G01 cells （with whole Bcr-Abl gene amplication） were tested. Kinase activity was measured using Kinase-GIo Luminescent Kinase Assay Platform in recombinant WT and mutant （Q252H, Y253F, and T3151） Abl kinases. Cell proliferation and apoptosis were examined using MTT assay and flow cytometry, respectively. The phosphorylation levels of Bcr-Abl initiated signaling proteins were analyzed using Western blotting. Colony forming units （CFU） growth and long term culture-initiating cells （LTC-ICs） were used to test the effects of C817 on human leukemia progenitor/stem cells. Results： C817 potently inhibited both WT and mutant （Q252H, Y253F, and T3151） Abl kinase activities in a non-ATP competitive manner with the values of ICso at low nanomole levels. In consistent with above results, C817 suppressed the growth of both imatinib-sensitive and resistant CML cells, including wild-type K562, K562/GO$, 32D-T3151, 32D-Q252H, and 32D-Y253F cells with the values of ICso at low micromole levels. C817 （0.5 or 1 tJmol/L） dose-dependently inhibited the phosphorylation of Bcr-Abl and downstream proteins STAT-5 and CrkL in imatinib-resistant K562/G01 cells. Furthermore, C817 significantly suppressed CFU growth and LTC-ICs, implicating that C817 could eradiate human leukemia progenitor/stem cells. Conclusion： C817 is a promising compound for treatment of CML patients with Bcr-Abl kinase domain mutations that confer imatinib resistance.
The recently proposed weak form quadrature element method （QEM） is applied to flexural and vibrational analysis of thin plates The integrals involved in the variational description of a thin plate ...are evaluated by an efficient numerical scheme and the par- tial derivatives at the integration sampling points are then approximated using differential quadrature analogs. Neither the grid pattern nor the number of nodes is fixed, being adjustable according to convergence need. The C~ continuity conditions char- acterizing the thin plate theory are discussed and the robustness of the weak form quadrature element for thin plates against shape distortion is examined. Examples are presented and comparisons with analytical solutions and the results of the finite element method are made to demonstrate the convergence and computational efficiency of the weak form quadrature element method. It is shown that the present formulation is applicable to thin plates with varying thickness as well as uniform plates.
Aim:Carvacrol (2-methyl-5-isopropylphenol), a phenolic monoterpene in the essential oils of the genera Origanum and Thymus, has been shown to exert a variety of therapeutic effects. Here we examined ...whether carvacrol protected neuroblastoma SH-SY5Y cells against Fe2+ -induced apoptosis and explored the underlying mechanisms.Methods:Neuroblastoma SH-SY5Y cells were incubated with Fe2+ for 24 h, and the cell viability was assessed with CCK-8 assay. TUNEL assay and flow cytometric analysis were performed to evaluate cell apoptosis. The mRNA levels of pro-inflammatory cytokines and NF-κB p65 were determined using qPCR. The expression of relevant proteins was determined using Western blot analysis or immunofluorescence staining.Results:Treatment of SH-SY5Y cells with Fe2+ (50-200 μmol/L) dose-dependently decreased the cell viability, which was significantly attenuated by pretreatment with carvacrol (164 and 333 μmol/L). Treatment with Fe2+ increased the Bax level and caspase-3 activity, and decreased the Bcl-2 level, resulting in cell apoptosis. Furthermore, treatment with Fe2+ significantly increased the gene expression of IL-1β, IL-6 and TNF-α, and induced the nuclear translocation of NF-κB. Treatment with Fe2+ also significantly increased the phosphorylation of p38, ERK, JNK and IKK in the cells. Pretreatment with carvacrol significantly inhibited Fe2+ -induced activation of NF-κB, expression of the pro-inflammatory cytokines, and cell apoptosis. Moreover, pretreatment with carvacrol inhibited Fe2+ -induced phosphorylation of JNK and IKK, but not p38 and ERK in the cells.Conclusion:Carvacrol protects neuroblastoma SH-SY5Y cells against Fe2+ -induced apoptosis, which may result from suppressing the MAPK/JNK-NF-κB signaling pathways.
Aim: Carvacrol (2-methyl-5-isopropylphenol), a phenolic monoterpene in the essential oils of the genera Origanum and Thymus, has been shown to exert a variety of therapeutic effects. Here we examined ...whether carvacrol protected neuroblastoma SH-SY5Y cells against Fe super(2+)-induced apoptosis and explored the underlying mechanisms. Methods: Neuroblastoma SH-SY5Y cells were incubated with Fe super(2+) for 24 h, and the cell viability was assessed with CCK-8 assay. TUNEL assay and flow cytometric analysis were performed to evaluate cell apoptosis. The mRNA levels of pro-inflammatory cytokines and NF- Kappa B p65 were determined using qPCR. The expression of relevant proteins was determined using Western blot analysis or immunofluorescence staining. Results: Treatment of SH-SY5Y cells with Fe super(2+) (50-200 mu mol/L) dose-dependently decreased the cell viability, which was significantly attenuated by pretreatment with carvacrol (164 and 333 mu mol/L). Treatment with Fe super(2+) increased the Bax level and caspase-3 activity, and decreased the Bcl-2 level, resulting in cell apoptosis. Furthermore, treatment with Fe super(2+) significantly increased the gene expression of IL-1 beta , IL-6 and TNF- alpha , and induced the nuclear translocation of NF- Kappa B. Treatment with Fe super(2+) also significantly increased the phosphorylation of p38, ERK, JNK and IKK in the cells. Pretreatment with carvacrol significantly inhibited Fe super(2+)-induced activation of NF- Kappa B, expression of the pro-inflammatory cytokines, and cell apoptosis. Moreover, pretreatment with carvacrol inhibited Fe super(2+)-induced phosphorylation of JNK and IKK, but not p38 and ERK in the cells. Conclusion: Carvacrol protects neuroblastoma SH-SY5Y cells against Fe super(2+)-induced apoptosis, which may result from suppressing the MAPK/JNK-NF- Kappa B signaling pathways.
String similarity join(SSJ) is essential for many applications where near-duplicate objects need to be found. This paper targets SSJ with edit distance constraints. The existing algorithms usually ...adopt the filter-andrefine framework. They cannot catch the dissimilarity between string subsets, and do not fully exploit the statistics such as the frequencies of characters. We investigate to develop a partition-based algorithm by using such statistics.The frequency vectors are used to partition datasets into data chunks with dissimilarity between them being caught easily. A novel algorithm is designed to accelerate SSJ via the partitioned data. A new filter is proposed to leverage the statistics to avoid computing edit distances for a noticeable proportion of candidate pairs which survive the existing filters. Our algorithm outperforms alternative methods notably on real datasets.
Aim： Carvacrol （2-methyl-5-isopropylphenol）, a phenolic monoterpene in the essential oils of the genera Origanum and Thymus, has been shown to exert a variety of therapeutic effects. Here we examined ...whether carvacrol protected neuroblastoma SH-SY5Y cells against Fe2＋-induced apoptosis and explored the underlying mechanisms. Methods： Neuroblastoma SH-SY5Y cells were incubated with Fe2＋ for 24 h, and the cell viability was assessed with CCK-8 assay. TUNEL assay and flow cytometric analysis were performed to evaluate cell apoptosis. The mRNA levels of pro-inflammatory cytokines and NF-KB p65 were determined using qPCR. The expression of relevant proteins was determined using Western blot analysis or immunofluorescence staining. Results： Treatment of SH-SY5Y cells with Fe2＋ （50-200 pmol/L） dose-dependently decreased the cell viability, which was significantly attenuated by pretreatment with carvacrol （164 and 333 pmol/L）. Treatment with Fe2＋ increased the Bax level and caspase-3 activity, and decreased the Bcl-2 level, resulting in cell apoptosis. Furthermore, treatment with Fe2＋ significantly increased the gene expression of IL-1β, IL-6 and TNF-α, and induced the nuclear translocation of NF-KB. Treatment with Fe2. also significantly increased the phosphorylation of p38, ERK, JNK and IKK in the cells. Pretreatment with carvacrol significantly inhibited Fe2＋-induced activation of NF-KB, expression of the pro-inflammatory cytokines, and cell apoptosis. Moreover, pretreatment with carvacrol inhibited Fe2＋-induced phosphorylation of JNK and IKK, but not p38 and ERK in the cells. Conclusion： Carvacrol protects neuroblastoma SH-SY5Y cells against Fe2＋-induced apoptosis, which may result from suppressing the MAPK/JNK-NF-KB signaling pathways.
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