Za svakog učenika koji završi devet godina osnovne škole veliki je korak u životu kada mora odlučiti što će raditi i što uopće želi raditi u životu. To je slučaj i nakon završetka srednje škole, iako ...je učenik pohađao strukovnu školu, za koju se opredijelio za stjecanje profesije do kraja četiri godine; u našem slučaju govorimo o srednjoj obrazovnoj školi koja vam nakon uspješno položene stručne mature daje i profesiju, a to je pomoćna učiteljica. Povezivanje vrtića i predškolskog obrazovanja sa srednjim strukovnim obrazovanjem od ključnog je značenja za kvalitetu obrazovanja učenika, posebno na početku njegove karijere. Uključivanje elemenata pažljivosti vrlo je važno. Utjecaj pažljivosti i razumijevanja može dovesti do boljeg odnosa i povezanosti između djeteta i odgojitelja. Koordinirana suradnja škole i vrtića s jedne je strane uspješna motivacija za školski rad učenika, a s druge strane dobrodošla diverzifikacija svakodnevne vrtićke rutine. Glazbena bajka predstavlja jedan od ciljeva Kurikuluma u vrtićima. To je tema učenja koja se stvara u fazama - od ideje, preko interdisciplinarnog povezivanja nastavnika, samostalnog rada učenika, do stvarne primjene krajnjeg proizvoda - izvođenja u vrtiću. Kada nastavnici u stvaranje glazbene bajke uključe elemente pažljivosti i preuzmu ulogu mentora u procesu, a učenici su skupina koja uči dizajnira bajku s naglašenom poučnom notom, uspjeh ne može izostati. O kvaliteti obavljenog posla najbolje svjedoče reakcije mališana - naše ciljne publike.
Different phytoestrogens were tested as inhibitors of 17β-hydroxysteroid dehydrogenase from the fungus
Cochliobolus lunatus (17β-HSDcl), a member of the short-chain dehydrogenase/reductase ...superfamily. Phytoestrogens inhibited the oxidation of 100
μM 17β-hydroxyestra-4-en-3-one and the reduction of 100
μM estra-4-en-3,17-dione, the best substrate pair known. The best inhibitors of oxidation, with IC
50 below 1
μM, were flavones hydroxylated at positions 3, 5 and 7: 3-hydroxyflavone, 3,7-dihydroxyflavone, 5,7-dihydroxyflavone (chrysin) and 5-hydroxyflavone, together with 5-methoxyflavone. The best inhibitors of reduction were less potent; 3-hydroxyflavone, 5-methoxyflavone, coumestrol, 3,5,7,4′-tetrahydroxyflavone (kaempferol) and 5-hydroxyflavone, all had IC
50 values between 1 and 5
μM. Docking the representative inhibitors chrysin and kaempferol into the active site of 17β-HSDcl revealed the possible binding mode, in which they are sandwiched between the nicotinamide moiety and Tyr212. The structural features of phytoestrogens, inhibitors of both oxidation and reduction catalyzed by the fungal 17β-HSD, are similar to the reported structural features of phytoestrogen inhibitors of human 17β-HSD types 1 and 2.
Different phytoestrogens were tested as inhibitors of 17β-hydroxysteroid dehydrogenase from the fungus
Cochliobolus lunatus (17β-HSDcl), a member of the short-chain dehydrogenase/reductase ...superfamily. Phytoestrogens inhibited the oxidation of 100
μM 17β-hydroxyestra-4-en-3-one and the reduction of 100
μM estra-4-en-3,17-dione, the best substrate pair known. The best inhibitors of oxidation, with IC
50 below 1
μM, were flavones hydroxylated at positions 3, 5 and 7: 3-hydroxyflavone, 3,7-dihydroxyflavone, 5,7-dihydroxyflavone (chrysin) and 5-hydroxyflavone, together with 5-methoxyflavone. The best inhibitors of reduction were less potent; 3-hydroxyflavone, 5-methoxyflavone, coumestrol, 3,5,7,4′-tetrahydroxyflavone (kaempferol) and 5-hydroxyflavone all had IC
50 values between 1 and 5
μM. Docking the representative inhibitors chrysin and kaempferol into the active site of 17β-HSDcl revealed the possible binding mode, in which they are sandwiched between the nicotinamide moiety and Tyr212. The structural features of phytoestrogens, inhibitors of both oxidation and reduction catalyzed by the fungal 17β-HSD, are similar to the reported structural features of phytoestrogen inhibitors of human 17β-HSD types 1 and 2.
Different phytoestrogens were tested as inhibitors of 17beta-hydroxysteroid dehydrogenase from the fungus Cochliobolus lunatus (17beta-HSDcl), a member of the short-chain dehydrogenase/reductase ...superfamily. Phytoestrogens inhibited the oxidation of 100 microM 17beta-hydroxyestra-4-en-3-one and the reduction of 100 microM estra-4-en-3,17-dione, the best substrate pair known. The best inhibitors of oxidation, with IC(50) below 1 microM, were flavones hydroxylated at positions 3, 5 and 7: 3-hydroxyflavone, 3,7-dihydroxyflavone, 5,7-dihydroxyflavone (chrysin) and 5-hydroxyflavone, together with 5-methoxyflavone. The best inhibitors of reduction were less potent; 3-hydroxyflavone, 5-methoxyflavone, coumestrol, 3,5,7,4'-tetrahydroxyflavone (kaempferol) and 5-hydroxyflavone all had IC(50) values between 1 and 5 microM. Docking the representative inhibitors chrysin and kaempferol into the active site of 17beta-HSDcl revealed the possible binding mode, in which they are sandwiched between the nicotinamide moiety and Tyr212. The structural features of phytoestrogens, inhibitors of both oxidation and reduction catalyzed by the fungal 17beta-HSD, are similar to the reported structural features of phytoestrogen inhibitors of human 17beta-HSD types 1 and 2.
Different phytoestrogens were tested as inhibitors of 17beta-hydroxysteroid dehydrogenase from the fungus Cochliobolus lunatus (17beta-HSDcl), a member of the short-chain dehydrogenase/reductase ...superfamily. Phytoestrogens inhibited the oxidation of 100microM 17beta-hydroxyestra-4-en-3-one and the reduction of 100microM estra-4-en-3,17-dione, the best substrate pair known. The best inhibitors of oxidation, with IC(50) below 1microM, were flavones hydroxylated at positions 3, 5 and 7: 3-hydroxyflavone, 3,7-dihydroxyflavone, 5,7-dihydroxyflavone (chrysin) and 5-hydroxyflavone, together with 5-methoxyflavone. The best inhibitors of reduction were less potent; 3-hydroxyflavone, 5-methoxyflavone, coumestrol, 3,5,7,4'-tetrahydroxyflavone (kaempferol) and 5-hydroxyflavone, all had IC(50) values between 1 and 5microM. Docking the representative inhibitors chrysin and kaempferol into the active site of 17beta-HSDcl revealed the possible binding mode, in which they are sandwiched between the nicotinamide moiety and Tyr212. The structural features of phytoestrogens, inhibitors of both oxidation and reduction catalyzed by the fungal 17beta-HSD, are similar to the reported structural features of phytoestrogen inhibitors of human 17beta-HSD types 1 and 2.
