The 239Pu, 238U, and 241Am concentrations and 239Pu/240Pu, 235U/238U, and 236U/238U atom ratios were measured in the hair and nail samples from individuals who donated their bodies to the United ...States Transuranium and Uranium Registries. Further, the concentration of 239Pu ranged from 0.22–15.8 ng/kg. The 240Pu/239Pu isotopic ratios ranged from 0.026 to 0.127 which is consistent with weapons-grade plutonium. Concentration of uranium fell between 1.84 µg/kg and 29.5 µg/kg and 235U/238U ratios ranged from 4.8×10-3 to 7.6×10-3. Elevated 236U/238U atom ratios were measured in two cases and ranged from 5.0×10-6 – 2.4×10-5 indicating exposure to spent or reprocessed uranium material. The concentration of 241Am was measured in four hair samples and ranged from 0.02–0.21 ng/kg.
The operation of a compact and busy preclinical laboratory comes with many challenges but also presents some surprising opportunities. Running a mouse and rat animal facility and full laboratory with ...a procedures room, multiple imaging modalities, radiochemical and cells lab, in a 100 m2 space has proven very functional from a managerial, organizational and multitasking aspect, as everything and everyone is close and easy to coordinate. Space limitations mainly affect washing, waste and supplies storage, adding heavy big equipment such as a biosafety cabinet, and hosting staff and occasional visitors. Operating the animal facility necessitates thorough sterilizations of cages and equipment in order to avoid cross-contamination due to receipt of animals from different breeders and health status. Procedure rooms are strategically organized to fit many needs, with the help of modern compact experimental equipment. The all in - all out operation, ability of close supervision, proximity of all laboratories, and good collaboration of everyone involved overcomes the confined space and allows for a rather convenient workflow. Radioprotection is addressed by using appropriate equipment and organizing the work accordingly, and exposure limits can be kept to the minimum for the public (1 mSv/year). Our experience proves that with good organization, resourcefulness and teamwork, less is more!
Recent studies suggest that ultrahigh-dose-rate, “FLASH,” electron radiation therapy (RT) decreases normal tissue damage while maintaining tumor response compared with conventional dose rate RT. ...Here, we describe a novel RT apparatus that delivers FLASH proton RT (PRT) using double scattered protons with computed tomography guidance and provide the first report of proton FLASH RT-mediated normal tissue radioprotection.
Absolute dose was measured at multiple depths in solid water and validated against an absolute integral charge measurement using a Faraday cup. Real-time dose rate was obtained using a NaI detector to measure prompt gamma rays. The effect of FLASH versus standard dose rate PRT on tumors and normal tissues was measured using pancreatic flank tumors (MH641905) derived from the KPC autochthonous PanCa model in syngeneic C57BL/6J mice with analysis of fibrosis and stem cell repopulation in small intestine after abdominal irradiation.
The double scattering and collimation apparatus was dosimetrically validated with dose rates of 78 ± 9 Gy per second and 0.9 ± 0.08 Gy per second for the FLASH and standard PRT. Whole abdominal FLASH PRT at 15 Gy significantly reduced the loss of proliferating cells in intestinal crypts compared with standard PRT. Studies with local intestinal irradiation at 18 Gy revealed a reduction to near baseline levels of intestinal fibrosis for FLASH-PRT compared with standard PRT. Despite this difference, FLASH-PRT did not demonstrate tumor radioprotection in MH641905 pancreatic cancer flank tumors after 12 or 18 Gy irradiation.
We have designed and dosimetrically validated a FLASH-PRT system with accurate control of beam flux on a millisecond time scale and online monitoring of the integral and dose delivery time structure. Using this system, we found that FLASH-PRT decreases acute cell loss and late fibrosis after whole-abdomen and focal intestinal RT, whereas tumor growth inhibition is preserved between the 2 modalities.
In this statement, the International Commission on Non-Ionizing Radiation Protection (ICNIRP) presents its principles for protection against adverse health effects from exposure to non-ionizing ...radiation. These are based upon the principles for protection against ionizing radiation of the International Commission for Radiological Protection (ICRP) in order to come to a comprehensive and consistent system of protection throughout the entire electromagnetic spectrum. The statement further contains information about ICNIRP and the processes it uses in setting exposure guidelines.
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