A cDNA encoding hyoscyamine 6β-hydroxylase (H6H, EC 1.14.11.11), a bifunctional enzyme catalyzing the last two steps in the scopolamine biosynthetic pathway, was isolated from Hyoscyamus senecionis, ...a medicinal plant endemic to the Iranian plateau. Expression analysis indicates that Hsh6h is expressed in all tested organs of H. senecionis including roots, rhizomes, leaves, stems and flowers unlike the other tropane alkaloid producing species. In parallel to this, in leaves, levels of scopolamine, the product of H6H, were higher than the substrate hyoscyamine. These data suggest that not only does the conversion of hyoscyamine to scopolamine take place in the root, followed by translocation to aerial parts, but also accumulated hyoscyamine in the aerial parts may be converted to scopolamine by activity of HsH6H. Analysis of expression profiles of putrescine N-methyltransferase and tropinone reductase I and II genes also indicates the organ-independent expression of these genes. Here we also introduce H. senecionis as an important tropane alkaloid producing species with its thick underground parts as a source of hyoscyamine, while its leaves can be considered as a source of scopolamine.
Display omitted Production of tropane alkaloids and expression of tropane alkaloid pathway genes were presented in Hyoscyamus senecionis, for the first time. Organ-independent expression of newly identified h6h cDNA and the other TA pathway genes explain an atypical pattern of scopolamine accumulation in this species.
•Cloning and characterization of a new H6H cDNA from Hyoscyamus senecionis.•An atypical pattern of tropane alkaloid accumulation and expression of TA pathway genes.•Hyoscyamine and scopolamine are mainly stored in rhizomes and leaves, respectively.
Glucose was found to exert an In vitro regulatory effect on prolactin secretion. Its role in the modulation of stimulated secretion of prolactin in man is, however, not clear. To evaluate the effect ...of hyperglycaemia on prolactin release, three stimulatory tests with different mechanisms of stimulation were employed. Healthy male subjects served as volunteers during submaximal exercise, TRH test (0.2 mg i.v.) and administration of haloperidol (2 mg i.v.). Glucose (100 g in 400 ml) or an equal volume of water was given 30 min before the tests. Blood for glucose and prolactin analysis was taken via an indwelling catheter. The plasma prolactin concentration increased in response to each of the stimuli applied. However, the prolactin increase during hyperglycaemia did not differ from values obtained in tests performed in normoglycaemia after water administration. These results indicate that prolactin release in healthy man is not modulated by hyperglycaemia.
A new bothriolepid antiarch, Vietnamaspis trii gen. et sp. nov., is described from the Middle-Upper Devonian sandstones of the Dô Son peninsula, near Haiphong, Vietnam. The site is north of the Song ...Ma suture zone on the South China Terrane, and contains a fauna including Bothriolepis and groenlandaspids. Vietnamaspis gen. nov. is characterized by having a long, parallel-sided posterior median dorsal plate, and an anterior median dorsal plate with a narrow anterior margin. Vietnamaspis gen. nov. is regarded as a sister taxon to Bothriolepis and Grossilepis because it has a relatively large axillary foramen on the anterior ventrolateral plate. The Australian bothriolepid Bothriolepis warreni Long 1983 is referred to a new genus, Briagalepis gen. nov., because it has very short overlap surfaces for the anterior dorsolateral plate on the anterior median dorsal plate, and lacks a reticulate dermal ornament.
La celebrazione della radio con tutti i personaggi che l'hanno resa grande e famosa.
1 - bambine fanno ginnastica secondo le indicazioni del programma radio;
2 - una massaia prende nota delle ricette ...trasmesse per radio;
3 - speaker annuncia l'orchestra moderna;
4 - sulle onde di un valzer la storia di due giovani vicini di casa che per vedersi si arrampicano sulla scala,
5 - la ragazza scoperta, viene allontanata dalla matrigna;
6 - la madre del ragazzo spegne la radio;
7 - la storia di un cacciatore raccontata alla radio;
8 - fiction sulla storia del cacciatore: i cannibali attaccano il cacciatore;
9 - i bambini ascoltano attentamente la storia trasmessa da un amplificatore;
10 - un annunciatore interrompe la trasmissione per comunicare la scomparsa di una bambina di due anni e mezzo;
11 - i piccoli balilla si organizzano in squadre per ritrovare la bambina scomparsa;
12 - i ragazzi si dividono e cercano nei parchi romani;
13 - i balilla ritrovano la bambina in un parco e la riportano a casa;
14 - un marinaio prende nota del bollettino meteorologico trasmesso per radio, mentre intorno infuria una mareggiata;
15 - marinai al lavoro sul peschereccio;
16 - l'orchestra sinfonica dell'EIAR accompagna le notizie;
17 - riprese aeree di cascate e di tramonti marini;
18 - sullo sfondo delle note dell'orchestra sinfonica dell'EIAR scorrono le immagini della natura in ogni sua manifestazione;
19 - ai piedi delle montagne procede il lavoro dei contadini accompagnato dalle voci della radio;
20 - ragazzi ascoltano, in classe, la radio con le note del trillo del Diavolo;
21 - fiction che descrive l'incontro di Giuseppe Tartini, il violinista, con il diavolo, da cui nacque il famoso brano;
22 - in alta montagna si festeggia il natale;
23 - gli operai, durante la pausa della mensa, ascoltano i programmi radio;
24 - testata del Radiocorriere;
25 - il maestro La Rosa Parodi esegue la sinfonia del Guglielmo Tell di Rossini;
26 - banco di manovra dell'impianto di trasmissione radio;
27 - spiegazione del trasmettitore;
28 - panoramica della sala a filtri elettrici di un trasmettitore ad onda corta,
29 - conduttore che trasporta l'energia ai quattro aerei direttivi;
30 - trasmettitore radio;
31 - lavoro nella redazione del giornale radio;
32 - radiocronaca di una partita;
33 - trasmissione dell'Anello del Nibelungo dal teatro reale dell'opera; ;
34 - rappresentazione scenica dell'opera di Wagner;
35 - locandina che annuncia , nella stagione lirica dell'EIAR, la tragedia lirica "il Poliuto" di Cammarano con musica di Donizetti;
36 - famiglia di contadini ascolta rapita l'opera attraverso la radio;
37 - coro e orchestra dell'Eiar diretta da La Rosa Parodi;
38 - coppietta amoreggia sulle rive del fiume e.. il gatto bianco opera il furto;
39 - il ladro, Nunzio Filogamo, con il suo complice, Fausto Tomei, si accorge di aver rubato, non alimenti, ma fotografie di donne;
40 - i due vengono catturati dal salumiere che vuole un autografo;
41 - il trio Lescano sceneggia la propria canzone;
42 - le tre ragazze si innamorano di tre garzoni e tutto finisce con il matrimonio;
43 - il maestro barzizza suona davanti alle stanze delle Lescano;
44 - Tomei e Filogamo continuano a mangiare dal salumiere;
45 - Ebe De Paulis canta rigovernando la cucina;
46 - Maria Bonelli intenta al ricamo;
47 - Romigioli e Vanni cantano lavando il pavimento;
48 - M.Pia Arcangeli canta mentre pulisce le patate;
49 - Niny Serena canta mettendo a posto la biancheria;
50 - Michele Montanari canta mentre pulisce gli argenti;
51 - Alfredo Chierici, canta mentre cucina;M.Luisa Dell'Amore canta alla cassa;
52 - Giovanni D'Ovidio canta portando in tavola le pietanze;
53 - Maria Jottini e Gilberto Mazzi cantano sotto la pioggia;
54 - Otello Focaccini e Lina Termini a spasso sulla carrozzella;
55 - esibizione dell'orchestra Seracini presentata da Nunzio Filogamo;
56 - maestro Petralia dirige l'orchestra;
57 - Barzizza dirige l'orchestra Cetra;
58 - il maestro Angelini e la sua orchestra;
59 - coppie sul palco accanto alle radio.
26 - radio transmission plant maneuver;
4 - on the waves of a waltz the story of two young neighbors who to see each other climb the staircase,
51 - Alfredo Chierici, sings while cooking; M. Luisa of love sings at the Cassa;
55 - performance of the Seracini orchestra presented by Nunzio Filogamo;
2 - a housewife notes the recipes broadcast for radio;
36 - family of peasants listen to the work kidnapped through the radio;
5 - the girl discovered, is removed from her stepmother;
20 - boys listen, in class, to the radio with the notes of the devil's trill;
6 - the boy's mother turns off the radio;
59 - couples on stage next to the radios.
58 - master Angelini and his orchestra;
57 - Barzizza conducts the cetra orchestra;
56 - Maestro Petralia conducted the orchestra;
46 - Maria Bonelli intent on embroidery;
45 - EBE de Paulis sings by regoverning the kitchen;
41 - the Lescano trio sets their own song;
32 - radiocronaca of a match;
31 - I work in the editorial staff of the radio newspaper;
30 - radio transmitter;
3 - speaker announces modern orchestra;
27 - explanation of the transmitter;
24 - RadioCorriere header;
22 - in the high mountains the birthplace is celebrated;
15 - sailors at work on the fishing boat;
48 - M.Pia Arcangeli sings while cleaning potatoes;
54 - Othello Focaccini and Lina Termini walking on the pram;
39 - the thief, Nunzio Filogamo, with his accomplice, Fausto Tomei, realizes that he has stolen, not foods, but photographs of women;
38 - Coppietta loves on the banks of the river e. the white cat operates theft;
21 - fiction describing the meeting of Giuseppe Tartini, the violinist, with the devil, from whom the famous passage was born;
8 - fiction about hunter history: cannibals attack hunter;
19 - at the foot of the mountains the work of the peasants accompanied by the voices of the radio proceeds;
17 - aerial shots of waterfalls and sea sunsets;
53 - Maria Jottini and Gilberto Mazzi sing in the rain;
18 - in the background of the notes of the Eiar Symphony orchestra, the images of nature in each of its manifestations flow;
34 - stage representation of Wagner's work;
42 - the three girls fall in love with three gauzes and everything ends with marriage;
44 - Tomei and Filogamo continue to eat from the charcuterie;
10 - an announcer interrupts the transmission to communicate the disappearance of a two-and-a-half year old girl;
23 - the workers, during the canteen break, listen to the radio programs;
50 - Michele Montanari sings while cleaning the silvers;
9 - children listen carefully to the story transmitted by an amplifier;
33 - transmission of the Nibelungo ring from the Royal opera house;;
35 - poster announcing, in the opera season of Eiar, the opera tragedy\ "Il Poliuto\" by CAMMARANO with music by Donizetti;
7 - the story of a hunter told on the radio;
49 - Niny Serena sings putting linen in place;
16 - the Eiar Symphony orchestra accompanies the news;
37 - Eiar choir and orchestra conducted by La Rosa Parodi;
12 - boys divide and seek in Roman parks;
11 - the little Balillas organize themselves into teams to find the missing child;
52 - John of Ovid sings bringing the dishes to the table;
13 - the Balillas find the little girl in a park and take her home;
1 - girls do gymnastics according to the indications of the radio program;
40 - the two are captured by the charmer who wants an autograph;
29 - conductor carrying energy to the four directive aircraft;
14 - a sailor notes the weather report transmitted by radio, while a storm rages around;
47 - Romigioli and Vanni sing washing the floor;
43 - master Barzizza plays in front of the rooms of the Lescano;
28 - overview of the electric filter room of a short wave transmitter,
The radio celebration with all the characters that made it great and famous.
25 - master La Rosa Parodi performs the Symphony of Rossini's William tell;
7 - official prize draw of the radiofortune contest
6 - radiofortune contest subscription booklets
4 - radio recording room: orchestra with the aurora trio perform a song
Tyrone power speaks at the ...microphone of the radio
Fiat Mickey prize of the competition
5 - recording of a classical music concert conducted by master Mario Rossi
2 - radio reporter of a football match
3 - Italian and foreign film and radio world characters speak at the microphone
1 - excerpt of a cycling race with the winner speaking at the microphone
1 - stralcio di una corsa ciclistica con il vincitore che parla al microfono
2 - radiocronista di una partita di calcio
Tyrone Power parla al microfono della radio
3 - personaggi del mondo del cinema e della radio italiani e stranieri parlano al microfono
4 - sala di registrazione della radio: orchestra con il trio aurora eseguono una canzone
5 - registrazione di un concerto di musica classica diretto dal maestro Mario Rossi
6 - libretti di abbonamento del concorso radiofortuna
7 - estrazione ufficiale dei premi del concorso radiofortuna
Fiat Topolino premio del concorso
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