One of the major problems in intensive breeding of chickens is liver damage. The objective of this study was to determine the influence of dietary supplementation with propolis and bee pollen on ...liver pathology in broiler chickens. The study was conducted on 200 Ross 308 chickens equally distributed by sex that were divided into five groups. Throughout the whole study, the control group of chickens was fed with a basal diet, while the experimental groups of chickens were fed with the same diet further supplemented with propolis and bee pollen, each supplement given separately or in combination in a certain proportion. The study showed that the clusters of lymphocytes in the hepatocytes, the vacuolar degeneration and necrosis of the liver parenchyma, the bile ductule hyperplasia, and the various forms of pathological changes in the liver arteries and veins were more frequent in liver tissue samples of the control group compared to liver tissue samples of all the experimental groups (
< 0.001). The study further showed that all the previously mentioned histopathological lesions of liver tissue were always more extensive in the liver tissue samples of the control group than in the liver tissue samples of all the experimental groups (
< 0.001). The supplementation of broiler chickens with propolis and/or bee pollen has a strong protective effect on liver pathology in broiler chickens.
Bee pollen and propolis are popular, traditional health foods. The objective of the current study was to investigate the anti-mutagenic, anti-histopathologic and antioxidant effects among water ...extracts of Egyptian bee pollen (WEBP) and brown powder of water-soluble derivative propolis (WSDP) on cisplatin (CDDP) induced hepatic, renal, testicular and genotoxicity in male albino mice (
Mus
muscullus
), in addition to their effects on the oxidant/antioxidant status in the tested organs. Hepatic, renal and testicular dysfunctions were evaluated histologically; while genotoxicity and cytotoxicity were evaluated by the bone marrow chromosomal aberration assay and mitotic index, respectively. Moreover, oxidative stress was explored via determination of lipid peroxidation, catalase activity and the concentration of the reduced form of glutathione. The treatment of mice with WEBP and WSDP at doses 140 and 8.4 mg/kg b. wt./day, respectively for 14 days simultaneously with CDDP (2.8 mg/kg b. wt.) resulted in significant protection. The positive control animals taken CDDP alone showed toxic histological and genetical manifestations (at
P
< 0.05) accompanied with an elevated content of peroxidized lipid and lowered catalase activity and glutathione concentration in the homogenate of liver, kidney and testis tissues (at
P
< 0.001). These toxic side effects in all tested organs were greatly ablated with a significant reduction in lipid peroxidation level and elevation in catalase activity and glutathione concentration (
P
< 0.001) when using both WEBP and WSDP. On the basis of the present assays, Bee pollen appears more potent in exerting an ameliorative effect and this effect was more pronounced in testis.
The aim was the evaluation of meat performance in chickens Ross 308 after the addition of bee pollen and propolis in a combination with probiotic into diet for broiler chicken. A total of 180 Ross ...308 broiler chickens of male and female sex were randomly divided into 3 groups (n=60): the control group (C) without additional supplementation, experimental group E1 supplemented with 400 mg bee pollen extract per 1 kg of feed mixture and 3.3 g probiotic (Lactobacillus fermentum) added to drinking water on a daily basis, and experimental group E2 supplemented with 400 mg propolis extract/1 kg feed mixture and 3.3 g probiotic (L. fermentum) added to drinking water on a daily basis. The feed mixtures were produced without any antibiotics and coccidiostatics. The fattening period lasted for 42 days. The findings of the work on the meat performance and carcass characteristics of chickens revealed that bee pollen in combination with probiotic was the most suitable feed supplement. Among the most noteworthy parameters affected positively (P≤0.05) by this supplement in comparison with control may be mentioned the breast part weight. Moreover, it seems likely that there was synergistic effect of bee pollen and probiotic manifested by higher live body and carcass weight (P≤0.05) in comparison with the control. Present results would also seem to suggest that propolis supplementation with probiotic had effects on meat performance and carcass characteristics of Ross 308 broiler chicken.
<span style="font-size:12.0pt;font-family: "Times New Roman","serif";mso-fareast-font-family:"Times New Roman";mso-ansi-language: ...EN-US;mso-fareast-language:SK;mso-bidi-language:AR-SA;mso-no-proof:yes" lang="EN-US">The study aimed to investigate the impact of Slovak bee pollen as supplement dietary in different doses (1000, 1500, 2500, 3500 and 4500 mg.kg-1 of feed mixture) on the sensory quality of broiler chickens. The study was carried out 180 one day-old chickens, which were divided into 6 groups (n=30). From each halves were separately sensory evaluated part from a thigh and breast. <span style="font-size:12.0pt;font-family:"Times New Roman","serif";mso-fareast-font-family: "Times New Roman";mso-ansi-language:SK;mso-fareast-language:SK;mso-bidi-language: AR-SA;mso-bidi-font-weight:bold;mso-no-proof:yes">Samples of heat treated meat were evaluated by a 6 member semi-trained panel of laboratory co-workers. Panelist <span style="font-size:12.0pt;font-family:"Times New Roman","serif"; mso-fareast-font-family:"Times New Roman";mso-ansi-language:SK;mso-fareast-language: SK;mso-bidi-language:AR-SA;mso-no-proof:yes">s evaluate aroma, juiciness, taste and tenderness on 5 point hedonic scale where 1 (the worst) and 5 (the best) were the extremes of each characteristic. The values of <span style="font-size:12.0pt;font-family:"Times New Roman","serif";mso-fareast-font-family: "Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:SK;mso-bidi-language: AR-SA;mso-no-proof:yes"> aroma, taste, juiciness and tenderness in breast and thigh muscles were higher in experimental groups in compare to control. The bee pollen has a positive impact on the taste, aroma, juiciness and tenderness of chickens thighs and breasts. Although the value of shear force in chicken thigh was significantly highest in E2 samples, addition of bee pollen to the diet for broiler chickens had no significatly negative effect on the thigh tenderness. Baking losses, as the second technological parameter, were also not significantly affected by nutrition with bee pollen supplement.
In this study, microwave drying (MWD) and microwave-assisted vacuum drying (MW-VD) (500, 675 mbar) at power levels of 300, 450, 600 and 900 W, hot-air drying (HAD) and vacuum drying (VD) (300, ...500 mbar) at temperatures of 35, 50, 65 °C and freeze drying (FD) of bee-pollen were investigated in relation to its total phenolic content (TPC), antioxidant vitamin composition (C and E) and antioxidant capacity. HAD at 35 °C provided similar vitamin C content as compared to fresh pollen while all other treatments led to significant loss ranging between 14.3 and 61.3%. Retention of vitamin E level in dried pollen ranged between 71 and 87%. Except vitamin C, MWD at lower power levels provided better or similar values of above mentioned properties as compared to HAD and FD treatments. The data indicated that pollen dried by MW-VD had higher antioxidant activity compared to samples dried by HAD irrespective of the applied pressure or power level.
In the brain, propionic acid (PA) can cross cell membranes and accumulate within cells, leading to intracellular acidification, which may alter neurotransmitter release (NT), communication between ...neurons, and behavior. Such elevation in levels of PA constitutes a neurodevelopmental metabolic disorder called propionic acidemia, which could clinically manifest as autism. The purpose of this study was to investigate the protective effects of different fractions of bee pollen (BP) on PA‐induced autism in rats, and to evaluate their effects on the expression of liver and renal biomarkers. Groups of rats received treatments of different fractions of BP at a dose of 250 mg/kg of body weight/day for a period of 1 month. Normal control group I and group II were orally administered with phosphate‐buffered saline and propionic acid, respectively, for 3 days. BP contains various health‐promoting phenolic components. Different fractions of BP administered pre‐ and post‐treatment with PA showed significant reduction in the levels of liver and renal biomarkers (p < .05). Also, a significant enhancement in the levels of glutathione S‐transferase (GST), catalase CAT), and ascorbic acid (VIT C) was observed. Supplementation with BP significantly reduced biochemical changes in the liver, kidneys, and brain of rats with PA‐induced toxicity. It exhibited protective effects against oxidative damage and reactive oxygen species produced by PA‐induced adverse reactions in rats. Taken together, our study shows that BP possesses protective effects in PA‐induced liver and kidney damage.
Bee pollen is a mixture of flower pollen, nectar, honey, wax, enzymes, and bee secretions. Foraging honey bees collect pollen from plants and transport it to the beehive, where it is stored and used as food for the colony. The present study demonstrates the protective effect of bee pollen to minimize liver, kidney, and brain insufficiencies associated with propionic acid‐induced autism in rat model. Bee pollen contains high percentage of flavonoid and flavonoid glycoside, suggesting that these compounds might be responsible for the protective effects against PA‐induced hepatorenal and brain injuries as well as antioxidant effects.
Bee pollen, one of the economic bee products, is widely known as a nutritional food with many bioactivities that depend on many such factors as bee species, plant source and biogeography. For this ...study, bee pollen was collected from
, harvested from the flowers of mimosa (
L.) in the Chiang Mai province, Thailand. The sample was extracted in methanol (MeOH) and then sequentially partitioned with hexane, dichloromethane (DCM) and MeOH in order to isolate compounds depending on their polarities. The obtained extracts were then tested for their antioxidant and anti-tyrosinase activities through 1,1-diphyenyl-2-picrylhydrazyl (DPPH) assay and for/through inhibition of mushroom tyrosinase extract, respectively. The DCM partitioned extract of mimosa flower bee pollen (DCMMBP) provided the highest antioxidant activity, with an effective concentration at 50% (EC
) of 192.1 μg/mL, and was further fractionated by silica gel 60 column chromatography and Sephadex LH20 size exclusion chromatography. All fractions were tested for their antioxidant activity and analyzed for the chemical structure through nuclear magnetic resonance (NMR). The most active fraction (EC
of 121.3 μg/mL) was a mixture of compounds, but the isolated flavonoid, naringenin, had a negligible antioxidant activity. In contrast, all three partitioned extracts and pooled fractions after silica gel 60 column chromatography provided no anti-tyrosinase activity (IC
of > 500 μg/mL) and a very low percentage of tyrosinase inhibition (0–2.99 ± 3.18%), compared to kojic acid (IC
of 8.58 μg/mL and tyrosinase inhibition of 74.2 ± 1.25%).
Background: Bee pollen (BP) has a broad range of beneficial effects on health. The aim of this study was to examine the effect of BP on the oral environment, including the microbiome and ...antimicrobial peptides. Methods: C57BL/6J mice were randomly divided into two groups: control and BP. The BP group was fed with a 5% BP diet for 1 month. Swabs from the oral and buccal mucosa and samples of the intestinal stool were collected. Genomic DNA was extracted and the microbiome was analyzed via 16S rRNA sequencing. Results: BP inhibited the growth of P. gingivalis at a concentration of >2.5%. The metagenomic study showed that the abundance of genus Lactococcus was significantly elevated in the oral and intestinal microbiomes of the BP group when compared to those of the control group. Significant alterations in alpha and beta diversity were observed between the oral microbiomes of the two groups. The mRNA levels of beta-defensin-2 and -3 were significantly upregulated in the buccal mucosa of the BP group. Conclusion: A BP diet may have a beneficial effect on oral and systemic health by modulating the bacterial flora and antimicrobial peptides of the oral cavity. Further investigations are needed to clarify how a BP diet affects overall human health.
Today, more than 1500 tons of bee pollen are produced per year worldwide. Despite the importance of this niche within the apiculture industry, several aspects of the bee pollen production system have ...been poorly studied. The most suitable population size for productive colonies, as well as the efficacy of carbohydrate and protein supplementation, has raised many questions and divergent conclusions are drawn in the literature. This research attempts to elucidate some questions of the bee pollen production system by measuring the bee pollen production of colonies with different population sizes and feeding treatments. Our results suggest that mid-sized (between 19,000 and 30,000 bees) colonies seem to optimize productivity and low frequency of problems of crammed grid boards. Carbohydrate supplementation should be used regularly and protein supplementation must be restricted to periods of pollen scarcity. Although this information will be helpful for beekeepers engaged in pollen production, a great number of questions concerning bee pollen production remain without the deserved scientific analysis.
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