This review covers the applications of mass spectrometry with sample introduction in the liquid phase to the analysis of the oxidation products of DNA, proteins and lipids in vitro and in vivo. The ...analysis of antioxidant xenobiotics in biological systems is also covered. The review covers the period 1997–late 2002. The most commonly used mass spectrometric mode used in this area of study is electrospray mass spectrometry that may also include collision-induced dissociation (CID). The review summarises for each paper the important methodological aspects of the techniques used and, where appropriate, the importance of the observations made within the context of biology.
A case of allergic contact dermatitis caused by heavy duty rubber gloves was reported. On patch testing with three kinds of gloves used by the patient and with various rubber additives, he reacted ...with only the rubber gloves and with N-isopropyl-N'-phenyl-p-phenylenediamine (IPPD). These reactions continued for two weeks. Chemical analysis clarified that the gloves contained IPPD and styrenated phenol (SP) at levels of 177 and 1600μg/g, respectively. The analytical procedure was as follows : 1) extraction by shaking with acetone : chloroform (1 : 1) mixture at room temperature ; 2) fractionation by silica gel column chromatography, by using hexane, ether : hexane mixture of various ether contents (5, 10, 50%) and ether as eluants ; 3) clean-up by silica gel thin-layer chromatography, by using chloroform : benzene (10 : 9) mixture as a developing solvent ; 4) qualitative and quantitative analysis by gas chromatography (GC) on 3% SE-30 column and GC-mass spectrometry. From the results mentioned above, we considered that IPPD should be a causative agent in this case. Since some phenolic compounds have been identified as sensitizers and SP is a kind of them, SP is also anticipated to be causative. The possibility could not be ruled out, because SP was not applied to patch testing.
Edible insects are alternative sources of high protein. Southeast Asia, South America, Africa, and Europe have recorded edible insects and, especially in Europe and America, there are factories that ...produce insects as food. In some areas of China, there has also been the habit of eating insects since ancient times. Periplaneta americana is an insect with the homology of medicine and food. In recent years, its medicinal and nutritional functions have attracted extensive attention and research. Its adult powder has been certified as a health product. It not only contains a variety of proteins but also is rich in fatty acids. The composition and antioxidant function of adult powder extract was analyzed in this study. Using adult P. americana powder as the raw material, it was extracted with n‐Hexane, dichloromethane, and ethyl acetate, respectively. Gas chromatography–mass spectrometry (GC–MS) removed a total of 60 compounds, and many active components were extracted from P. americana powder for the first time. The parts and relative content of each extracted sample were obtained, and the total antioxidant capacity (T‐AOC) of each extracted piece was determined, among which the antioxidant activity of ethyl acetate was the highest.
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