Display omitted
•Simultaneous detection of multiple isotopologues with FT-IBBCEAS in the near-IR.•Experimental/spectroscopic characterization of combination and overtone bands of DONO.•Experimental ...detection and confident assignment of two new DNO3 bands.
The first measurements of near-infrared bands of deuterated nitrous acid (DONO) are presented. The measurements were made using Fourier-Transform Incoherent Broad-Band Cavity-Enhanced Absorption Spectroscopy (FT-IBBCEAS) in the 5800–7800 cm−1 region. Two bands of trans-DONO centred at 6212.029 and 7692.496 cm−1 were observed and assigned to the 2ν1+ν3 combination and 3ν1 overtone vibrations, respectively. Their rotational band structure was satisfactorily reproduced using PGOPHER. For cis-DONO the 3ν1 band was observed at ~7302.5 cm−1. In addition, new bands centred at 6142.5 cm−1 and 7607.6 cm−1 were quite confidently assigned to the 2ν1+ν3 and 3ν1 vibrations of deuterated nitric acid, DNO3, respectively.
In this interview with Heri Dono, the artist discusses his ongoing relationship to craft and traditional art in Indonesia. Dono is an artist who intentionally blurs the boundaries between fine art ...and craft to subvert artistic conventions. Born in 1960 in Jakarta and raised in Semarang, Central Java, Dono is among a generation of artists who came of age during the politically repressive era of Suharto, also known as the New Order (1966-1998). Dono studied painting at the Indonesian Institute of the Arts (ISI) in Yogyakarta between 1980 and 1987. He dropped out of art school to study the popular form of Javanese shadow puppetry known as wayang kulit, and became a professionally trained storyteller dalang (puppet master). That experience heavily inspired Dono's paintings, sculptures, installations, and performances, enabling him to develop his own personal aesthetic and visual language based on the characters depicted in the wayang. Dono regularly enlists craftsmen as well as untrained members of the local community to participate in his projects as a tool of empowerment. In pieces such as Wayang Legenda (1988) and Kuda Binal (1992), Dono drew upon traditional modes of storytelling and ritual dancing as a subtle form of social critique and political commentary.
Under global modernity, Southeast Asian shadow puppet theatre is being re-worked in art galleries, the internet, community arts contexts, intermedial collaborations and festivals. Even while cultural ...conservatives mourn vanishing traditions, a generation of Southeast Asian practitioners are seizing the codes of performance culture, inhabiting received forms and re-making them to speak to both local particularities and global issues. These post-traditional artists mine traditions for political parody and carnivalesque revelry, violating the sacred aura of puppets to assert their own authorship and critique heritage discourse and development policies. Puppeteers fashion new performance genres, ephemeral creations re-negotiating connections between the local and the global. Animators and game designers re-work the figures, performance dynamics, and stories of shadow play traditions in digital milieus. Such abductions and radical interpretations stoke debates about cultural identity and patrimony, aesthetic norms and moral values, individual agency and collective creativity, postcolonial exoticism and the politics of recognition.
: Background: The European Center for Validation of Alternative Methods (ECVAM) has funded a prevalidation study in three laboratories (France, USA and UK) on the use of human hepatocyte cultures to ...predict cytochrome P‐450 induction.
Aims and Methods: As first stage of this prevalidation study, the purpose of the present work was to set criteria for optimization and harmonization of hepatocyte isolation from human tissue among laboratories to establish a routine procedure. This was achieved by combining and/or comparing the data generated by the two independent European laboratories (France and UK).
Results: The results confirmed that surgical waste material is a valuable source for obtaining high quality hepatocytes under certain pre‐, intra‐ and post‐operative conditions: cell yield of viable hepatocytes was not significantly affected by age and sex of patients, nor indications for resection, steatosis or cholestasis. Cold ischeamia up to 5 hours did not influence viable cell yield allowing transport of material.
Conclusion: The use of biopsy sizes between 50–100 g, cannulation with 2–4 cannulae, digestion with collagenase‐containing digestion medium at a flow rate of 25 ml/cannula for 20 minutes, with cut surface being glued in order to reform Glisson's capsule, should optimize the total yield of viable human hepatocytes obtained per preparation of waste liver surgical resecitons.
In this paper, we present the first high-resolution (0.003
cm
−1) absorption measurements of the pure rotational spectra of nitrous acid (
trans- and
cis-HONO) and its deuterated species (
trans- and
...cis-DONO) in the far-infrared region between 40 and 150
cm
−1. The spectra were first assigned based on rotational constants from previous studies in the microwave and mid-infrared regions. New rotational and centrifugal distortion constants were determined for all four species. The accuracy of the principal rotational constants was improved, and several quartic and sextic (and even a few octic) centrifugal distortion constants were obtained for the first time. Synthetic spectra calculated using the new constants of this study reproduce the observed spectra very well.
PDF
