Changes in the values of biochemical and cellular blood constituents can indicate the presence of early pathology. The accuracy of a laboratory test depends, among other factors, on the quality of ...the sample provided for analysis. The aim of this study was to verify the feasibility of collected blood samples from cattle analyzed at different time intervals and report the variations observed at the values of haematological parameters. Ten whole blood samples of crossbred cattle, refrigerated (0-2°C), to achieve four blood counts in each sample unit, in different time 2, 26, 52 and 74 hours after collection were used. There have been changes in the values of VG from 26h, the total count of erythrocytes was the parameter that most ranged between the different times evaluated. Hemoglobin was increased in most samples in the analysis after 2h, probably due to the occurrence of hemolysis. Also there was an increase in the values of MCV in different time intervals. The results of the values of TPP also demonstrated abnormal in 26h after collection. However, the fibrinogen was the parameter that remained with more stable values in the different times evaluated. Only in periods of 52h and 74h morphological changes were visualized in leukocytes. It was concluded that there was wide variation in the values of the parameters evaluated between periods of 2h and 26h, if samples are preserved properly can be examined within 24h.
Alterações nos valores dos constituintes celulares e bioquímicos sanguíneos, podem indicar precocemente a presença de uma patologia. A exatidão de um teste laboratorial, além de outros fatores, depende da qualidade da amostra fornecida para análise. O objetivo do presente estudo foi verificar a viabilidade de amostras sanguíneas coletadas de bovinos analisadas em diferentes intervalos de tempo e relatar as variações observadas nos valores dos parâmetros hematológicos. Foram utilizadas 10 amostras de sangue total de bovinos mestiços, refrigeradas (0-2°C), para a realização de quatro hemogramas em cada unidade amostral em espaços de tempo diferentes 2, 26, 52 e 74 horas após a coleta. Houve alterações nos valores de VG a partir de 26h, a contagem global dos eritrócitos foi o parâmetro que mais variou entre os diferentes tempos avaliados. A dosagem de hemoglobina teve um aumento na maioria das amostras nas análises após 2h, provavelmente, devido à ocorrência de hemólise. Também ocorreu aumento nos valores de VGM nos diferentes intervalos de tempo. Os resultados dos valores de PPT também se apresentaram alterados em 26h após a coleta. Já o fibrinogênio foi o parâmetro que permaneceu com valores mais estáveis nos diferentes tempos avaliados. Apenas nos períodos de 52h e 74h foram visualizadas alterações morfológicas nos leucócitos. Concluiu-se que não houve grande variação nos valores dos parâmetros avaliados entre os períodos de 2h e 26h, se as amostras forem conservadas adequadamente podem ser examinadas em até 24h.
Changes in the values of biochemical and cellular blood constituents can indicate the presence of early pathology. The accuracy of a laboratory test depends, among other factors, on the quality of ...the sample provided for analysis. The aim of this study was to verify the feasibility of collected blood samples from cattle analyzed at different time intervals and report the variations observed at the values of haematological parameters. Ten whole blood samples of crossbred cattle, refrigerated (0-2°C), to achieve four blood counts in each sample unit, in different time 2, 26, 52 and 74 hours after collection were used. There have been changes in the values of VG from 26h, the total count of erythrocytes was the parameter that most ranged between the different times evaluated. Hemoglobin was increased in most samples in the analysis after 2h, probably due to the occurrence of hemolysis. Also there was an increase in the values of MCV in different time intervals. The results of the values of TPP also demonstrated abnormal in 26h after collection. However, the fibrinogen was the parameter that remained with more stable values in the different times evaluated. Only in periods of 52h and 74h morphological changes were visualized in leukocytes. It was concluded that there was wide variation in the values of the parameters evaluated between periods of 2h and 26h, if samples are preserved properly can be examined within 24h.
Alterações nos valores dos constituintes celulares e bioquímicos sanguíneos, podem indicar precocemente a presença de uma patologia. A exatidão de um teste laboratorial, além de outros fatores, depende da qualidade da amostra fornecida para análise. O objetivo do presente estudo foi verificar a viabilidade de amostras sanguíneas coletadas de bovinos analisadas em diferentes intervalos de tempo e relatar as variações observadas nos valores dos parâmetros hematológicos. Foram utilizadas 10 amostras de sangue total de bovinos mestiços, refrigeradas (0-2°C), para a realização de quatro hemogramas em cada unidade amostral em espaços de tempo diferentes 2, 26, 52 e 74 horas após a coleta. Houve alterações nos valores de VG a partir de 26h, a contagem global dos eritrócitos foi o parâmetro que mais variou entre os diferentes tempos avaliados. A dosagem de hemoglobina teve um aumento na maioria das amostras nas análises após 2h, provavelmente, devido à ocorrência de hemólise. Também ocorreu aumento nos valores de VGM nos diferentes intervalos de tempo. Os resultados dos valores de PPT também se apresentaram alterados em 26h após a coleta. Já o fibrinogênio foi o parâmetro que permaneceu com valores mais estáveis nos diferentes tempos avaliados. Apenas nos períodos de 52h e 74h foram visualizadas alterações morfológicas nos leucócitos. Concluiu-se que não houve grande variação nos valores dos parâmetros avaliados entre os períodos de 2h e 26h, se as amostras forem conservadas adequadamente podem ser examinadas em até 24h.
The most commonly performed blood test is complete blood cell count. This test includes hemoglobin, white blood cell count, platelet count, and detailed red blood cell indices. Automated complete ...blood count also give information for “differential” which gives information about percentages and absolute numbers of different subgroups of white blood cells. This test is necessary in diagnosing anemia, hematological cancers, infections, acute hemorrhagic states, allergies, and immunodeficiencies. Also it is used for monitoring side effects of certain drugs. A pediatrician is frequently challenged for evaluating complete blood count as a part patient’s assessment. An enhanced and complete understanding of this laboratory test is essential for providing quality care of sick and normal children. Here in this paper, we want to share key laboratory interpretation strategies for complete blood count and some clues for differentiating normal from deviations and true problems.
Objective. To evaluate the effect of biopreparations obtained from agroindustrial by-products fermented with lactic acid bacteria and yeasts on the bioproductive behavior of primiparous guinea pigs ...and their offspring. Materials and methods. A total of 40 nulliparous guinea pigs were used, with 125 d of age, 1450 g of live weight, distributed in four groups of 10 animals each. T1, Control (basal diet without additive); T2, substrate fermented with Lactobacillus acidophilus. T3, substrate fermented with Kluyveromyces fragilis and T4, substrate fermented with L. acidophillus and K. fragilis. A completely randomized design was used where weight gain during pregnancy, age at first delivery, percentage of fertility, conception index, health, and values of hemoglobin, hematocrit and mean corpuscular volume were evaluated. Results. Weight gain during pregnancy was greater (P <0.05) in T4; the age at first delivery was reduced (P <0.05) in Treatment 4; the fertility percentage and the conception index were better (P <0.05) in the animals that consumed the T4 biopreparation, the occurrence of diarrhea was lower (P <0.05) in the T4 group. Hematological values ware improved in all groups that consumed biopreparations. Conclusions. The use of agroindustrial substrates fermented with L. acidophilus and K. fragilis improved the productive and reproductive indicators in primiparous guinea pigs. In addition, it intervenes in the improvement of health and hematological values.
Objetivo. Evaluar el efecto de biopreparados obtenidos a partir de subproductos agroindustriales fermentadas con bacterias acido-lácticas y levaduras sobre el comportamiento bioproductivo de las cobayas primíparas y su descendencia. Materiales y Métodos. Se emplearon un total de 40 cobayas nulíparas, con 125 d de edad, 1450 g de peso vivo, repartidas en cuatro grupos de 10 cobayas cada uno. T1, Control (dieta basal sin aditivo); T2, sustrato fermentado con Lactobacillus acidophilus. T3, sustrato fermentado con Kluyveromyces fragilis y T4, sustrato fermentado L. acidophillus y K. fragilis. Mediante un diseño completamente aleatorizado se evaluó: ganancia de peso durante la gestación, edad al primer parto, el porciento de la fertilidad, índice de concepción, salud y los valores de hemoglobina, hematocrito, y volumen corpuscular media. Resultados. La ganancia de peso durante la gestación fue superior (P<0,05) en el T4; la edad al primer parto se redujo (P<0,05) en el Tramiento 4; el porciento de fertilidad e índice de concepción fue mejor (P<0.05) en los animales que consumieron biopreparado T4, la ocurrencia de diarrea fue menor (P<0,05) en el grupo T4. Los valores hematológicos se mejoraron en todos los grupos que consumieron biopreparados. Conclusión. El uso de sustratos agroindustriales fermentados con L. acidophilus y K. fragilis mejoraron los indicadores productivos y reproductivos en cobayas primíparas. Además, interviene en el mejoramiento de la salud y los valores hematológicos.
Aim: Allergic proctocolitis (AP) is a common cause of rectal bleeding in infants. There is no diagnostic tool specific to the disease. The aim of the study was to evaluate hemogram parameters as a ...marker of inflammation in patients with AP.Material and Methods: The files of patients who were exclusively breastfed and diagnosed as food protein induced AP were examined retrospectively. A hundred and fifty patients diagnosed with AP were included in the study. The same number of healthy babies formed the control group. Parameters in complete blood count were compared between patient and control groups. Rectosigmoidoscopic examination was performed to confirm the diagnosis in patients who could not achieve complete improvement with diet therapy, and hemogram parameters were evaluated according to eosinophilic infiltration in biopsies.Results: The mean platelet volume (MPV) values (p<0.001) and eosinophil percentages (p=0.001) of the AP group were higher than the control group. The mean hemoglobin values of the AP group were statistically significantly lower than the control group (p<0.001). No statistically significant difference was found between patient and control groups in terms of white blood cell (WBC) count, platelet count, platelet distribution width (PDW) and neutrophil/lymphocyte ratio (NLR). WBC count, platelet count, hemoglobin, MPV and C-reactive protein (CRP) values were similar between groups of patients according to the number of eosinophils in colon biopsies.Conclusion: We observed a significantly higher MPV values and eosinophil percentages in patients with AP. These parameters maybe helpful in diagnosis of AP.
Amaç: Alerjik proktokolit (AP), bebeklerde görülen rektal kanamanın yaygın bir nedenidir. Bu hastalığa özgü olan bir tanı aracı yoktur. Çalışmanın amacı, AP tanısı konmuş olan hastalarda inflamasyonun bir belirteci olarak hemogram parametrelerini değerlendirmektir.Gereç ve Yöntemler: Sadece anne sütü ile beslenen ve gıda proteini kaynaklı AP tanısı konan hastaların dosyaları geriye dönük olarak incelendi. AP tanısı konulmuş olan 150 hasta çalışmaya dahil edildi. Aynı sayıda herhangi bir sağlık sorunu saptanmayan bebek ise kontrol grubunu oluşturdu. Tam kan sayımındaki parametreler hasta ve kontrol grupları arasında karşılaştırıldı. Diyet tedavisi ile tam iyileşme sağlayamayan hastalarda tanıyı doğrulamak için ilave rektosigmoidoskopik inceleme yapıldı ve hemogram parametreleri biyopsilerdeki eozinofil infiltrasyon düzeyleri dikkate alınarak değerlendirildi.Bulgular: AP grubunun ortalama trombosit hacmi (mean platelet volume, MPV) değerleri (p<0.001) ve eozinofil yüzdeleri (p=0.001) kontrol grubundan daha yüksekti. AP grubunun ortalama hemoglobin değerleri kontrol grubundan istatistiksel olarak anlamlı derecede daha düşüktü (p<0.001). Hasta ve kontrol grupları arasında beyaz kan hücresi (white blood cell (WBC) sayısı, trombosit sayısı, trombosit dağılım genişliği (platelet distribution width, PDW) ve nötrofil/lenfosit oranı (NLR) açısından istatistiksel olarak anlamlı bir fark bulunmadı. WBC sayısı, trombosit sayısı, hemoglobin, MPV ve C-reaktif protein (CRP) değerleri, kolon biyopsilerindeki eozinofil sayısına göre hasta grupları arasında benzerdi.Sonuç: AP'li hastalarda anlamlı olarak yüksek MPV değerleri ve eozinofil yüzdeleri gözlendi. Bu parametreler AP tanısında yardımcı olabilir.
Inflammation, even overt or subtle, has an important role in the pathogenesis of various diseases. Hemogram derived markers are novel inflammatory predictors and have been studied by the authors in ...different settings, including gastrointestinal conditions. Of those, mostly used indices are mean platelet volume (MPV), red cell distribution width (RDW), neutrophil-to-lymphocyte ratio(NLR), platelet-to-lymphocyte ratio (PLR), and monocyte-to-lymphocyte ratio. Besides the well-established roles of these markers in various hematological issues, including differentiating anemia, platelet activation and infections, recent literature suggest that they could be associated with the inflammatory burden in gastrointestinal conditions from inflammatory disease to functional disorders and malignancies. In present work, we aimed to review the literature to reveal the association between these markers and gastrointestinal diseases to establish their diagnostic and prognostic role in disorders of the gastrointestinal system. In present work, we aimed to review the literature to reveal the association between these markers and gastrointestinal diseases to establish their diagnostic and prognostic role in disorders of the gastrointestinal system.
Objective: Following acute exercises, changes in mineral and hematological concentrations are observed in the organism depending on the exercise, but regular and submaximal exercises do not fully ...reveal the mineral and hematological effects. This study was conducted to determine the effects of volleyball training on mineral metabolism and hematological parameters over a period of three months.
Method:The research group included 20 healthy male volunteers in the 13-16 age group. A training program of 80 minutes per day, 4 days per week, for three months was applied to the athletes who participated in the study. Blood samples were taken twice before and at the end of the training sessions from the resting athletes. Sodium, potassium, chlorine, calcium, magnesium, WBC, RBC, HGB, HCT, MCV, MCH, MCHC, PLT, MPV and PDW levels were determined in the blood samples collected. The data were analyzed using the SPSS 22 package program. The Paired Samples t-test was used to compare the research group's pre-final test data. Significance was considered to be p>0.05.
Finding: The analysis showed that the research group had statistically significant differences in sodium, chlorine, phosphorus, magnesium, RBC, HCT, MCV, MCH, MCHC, MPV and PCT levels (p<0.05) and no statistically significant differences in potassium, calcium, WBC, HGB, PLT, RDW-CV and PDW levels (p>0.05).
Conclusion: As a result, volleyball training over a long period of time has been observed to cause changes in mineral metabolism and some hematological parameters. It was found that the regular training schedule caused differences in some mineral and whole blood values. In the light of this information, we believe that long-term adolescent training will have a positive impact on the health of athletes.
Bu çalışmada; neonatal ishalli buzağılarda farklı etiyolojik faktörlerin hemogram parametreleri üzerine etkisinin belirlenmesi amaçlandı. Araştırmada 1-20 günlük yaşta 44 ishalli, 18 sağlıklı toplam ...62 buzağı kullanıldı. Rektumdan alınan dışkı örneklerinde Clostridium perfringens, E. coli, Cryptosporidium, Rotavirus ve Coronavirus etkenlerine yönelik immunokromatografik test kitleriyle bakılıp buzağılar gruplandırıldı. Vena jugularis’den alınan kanlarda total lökosit, granülosit, lenfosit, monosit, eritrosit ve hematokrit parametreleri ölçüldü. Deney grubu buzağıların total lökosit, granülosit ve monosit düzeylerinin kontrol grubuna göre önemli düzeyde yüksek (p<0,05) olduğu saptandı. Lenfosit, eritrosit ve hematokrit düzeyleri yönünden ise kontrol ve deney grubu buzağılar arasında farklılık tespit edilmedi. İncelenen tüm hemogram bulguları yönünden alt deney gruplarındaki buzağıların kendi aralarında ve kontrol grubuyla arasındaki farkların önemsiz olduğu (p>0,05) belirlendi. Sonuç olarak hemogram parametrelerinin ishalli buzağılarda önem arz ettiği ancak etiyolojik faktörlere göre farklılık göstermediği görüldü. Bu değişikliklerin etiyolojik faktörlerin tipi, sayısı, hayvanın durumu, tablonun şiddeti/süresi gibi birçok faktörden etkilenebileceğinden dolayı değişiklik göstermediği kanısına varıldı. Konuyla ilgili daha fazla hayvan üzerinde ve çevresel faktörlerin kontrol altına alındığı çalışılmaların yapılması gerektiği görüldü.
In this study; It was aimed to determine the effect of different etiological factors on hemogram parameters incalves with neonatal diarrhea. A total of 62 calves, 44 with diarrhea and 18 healthy calves, aged 1-20 days were used in the study. Stool samples taken from the rectum were examined with immunochromatographic test kits for Clostridium perfringens, E. coli, Cryptosporidium, Rotavirus and Coronavirus factors and calves were grouped. Total leukocyte, granulocyte, lymphocyte, monocyte, erythrocyte and hematocrit parameters were measured in blood taken from vena jugularis. Total leukocyte, granulocyte and monocyte levels of the experimental groups were found to be significantly higher (p <0.05) compared to the control group. There was no difference between control and experimental groups in terms of lymphocyte, erythrocyte and hematocrit levels. It was determined that the differences was insignificant between the calves in the sub-experimental groups among themselves and with the control group (p> 0.05). As a result, it was seen that hemogram parameters were important in calves with diarrhea, but did not differ according to etiological factors. It was concluded that these changes did not alter since they may be affected by others factors such as the type and number of etiological factors, the animal's condition, the severity/duration of the diseases. It was seen that more studies on the subject and environmental factors should be studied.
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