•Were obtained 485.7mg of FAg−1 of alpha-linolenic acid from extraction of linseed oil via SubFE.•TAG profiles was obtained using ESI-MS and higher purity was found for the SubFE oil.•By DSC ...analysis, linseed oil extracted at 45°C and 10MPa resisted 89.5min without oxidation.•Were quantified campesterol, stigmasterol and sitosterol in all oils.
Flaxseed (Linum usitatissimum L.) oil was obtained via subcritical n-propane fluid extraction (SubFE) under different temperatures and pressures with an average yield of 28% and its composition, purity and oxidative stability were compared to oils obtained via conventional solvent extraction methods (SEMs). When the oxidative stability was measured by differential scanning calorimetry, the oil was found to be up to 5 times more resistant to lipid oxidation as compared to the SEM oils. Direct infusion electrospray ionization mass spectrometry (ESI-MS) analysis showed characteristic and similar TAG profiles for SubFE and SEMs oils but higher purity for the SubFE oil. The flaxseed oil content of β-tocopherol, campesterol, stigmasterol and sitosterol were quantified via GC–MS. SubFE showed to be a promising alternative to conventional SEM since SubFE provides an oil with higher purity and higher oxidation stability and with comparable levels of biologically active components.
Flaxseed oil (FO) has been demonstrated its multiple beneficial effects in vivo due to high concentration of α-linolenic acid. The deterioration of FO can be triggered by high temperature heating ...during the deep frying process resulting in alteration of healthy properties. In this study, the effect of FO before and after deep frying on lipid metabolism and gut homeostasis of rats was investigated compared to deep-fried palm oil (DPO) treated group. Deep-fried flaxseed oil (DFO) treatment significantly enhanced the triglyceride accumulation in serum and liver tissues of rats. A greater increase of peroxides and proinflammatory cytokine levels was found in the serum of DFO treated rats compared to other groups. The histopathologic data indicated that DFO and DPO reduced the villus height of intestinal and colonic tissues and increased the inflammatory cell infiltration. The inflammatory cytokines (TNFα and IL-6) were enhanced and the key markers of epithelia colonic tissues (occludin and MUC-2) were suppressed in rats with DFO interventions, which is in consistency with histopathologic results. In addition, FO could increase the number of beneficial bacteria while the relative abundance of obesity and inflammatory-related bacteria was promoted by DFO treatment, including Ruminococcaceae, Prevotellaceae, and Selenomonadales. In conclusion, DFO intake had a significant impact on the disruption of gut barrier homeostasis, potentially worsening the dysbiosis than DPO. The beneficial effects of FO in vivo could be significantly reduced by extreme deep frying, which suggests the need for moderate cooking edible oils such as FO.
The aim of this study was to investigate the effects of dietary supplementation with heated linseed on the fatty acid (FA) composition of the plasma, liver, and subcutaneous adipose tissue (SADT) of ...Albas white cashmere kids, particularly the effect on n-3 long-chain polyunsaturated FA profiles and the mRNA expression of genes related to lipid metabolism in SADT. Sixty 4-month-old castrated male kids (average BW 18.6 ± 0.1 kg) were selected and randomly allocated into three groups in a randomized block design. Three dietary treatments were used: (1) basal diet without supplementation (Control), (2) basal diet supplemented with linseed oil (LSO), and (3) basal diet supplemented with heated linseed grain (HLS). The diets were fed for 104 d, consisting of 14 d for adaptation followed by 90 d of measurement. Different FA profiles were found in SADT between LSO and HLS. Kids fed HLS had more C18:3n3 (P < 0.0001), C22:6n3 (P = 0.007), and n-3 PUFA (P < 0.0001) and a less (P < 0.0001) n-6/n-3 ratio than LSO kids. These FA differences between LSO and HLS kids were due to the increased expression of elongation of very long chain FA protein 5 (P < 0.0001), delta-6 desaturase (P < 0.0001), and peroxisome proliferator-activated receptor α (P = 0.003) in SADT of HLS kids and was also associated with liver fat metabolism. Together, these results suggest that the consumption of HLS leads to more C22:6n3 than LSO in SADT by increasing liver C22:6n3 content and by increasing SADT mRNA expression of ELOVL5 and FADS2 through promoting PPARα expression.
► We set up an effective and clean way to extract flaxseed oil. ► Aqueous enzymatic process assisted by ultrasound extraction method was optimized. ► Kinetic parameters of the free and immobilized ...enzymes were studied. ► Oil shows good quality.
An aqueous enzymatic process assisted by ultrasound extraction (AEP-UE) was applied to the extraction of oil from flaxseed (Linum usitatissimum L.). The highest oil recovery of 68.1% was obtained when ground flaxseed was incubated with 130U/g of cellulase, pectinase, and hemicellulase for 12h, at 45°C and pH 5.0. The IC50 values of oil obtained by AEP-UE and organic solvent extraction (OSE), as measured by DPPH scavenging activity essay, were 2.27mg/mL and 3.31mg/mL. The AEP-UE-derived oil had a 1.5% higher content of unsaturated fatty acids than the OSE-derived oil. AEP-UE is therefore a promising environmentally friendly method for large-scale preparation of flaxseed oil.
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•Pea protein (PP) and tannic acid (TA) were used to form physical complexes.•High TA levels promoted heat-induced aggregation of complexes and emulsions.•PP-TA complexes protected the ...flaxseed oil emulsions from droplet aggregation.•PP-TA complexes protected the flaxseed oil emulsions from lipid oxidation.•PP-TA complexes did not adversely affect the digestion of the emulsions.
There is growing interest in the identification of plant-based functional ingredients for utilization within the food industry. Complexes were fabricated from pea protein (PP) and tannic acid (TA) and then their ability to act as antioxidant emulsifiers in flaxseed oil-in-water emulsions was studied. PP-TA complex formation was investigated using isothermal titration calorimetry and turbidity analysis, which suggested hydrogen bonding and hydrophobic interactions were important in their assembly. PP-TA-stabilized emulsions containing small droplets could be formed at relatively high TA levels. Moreover, PP-TA complexes had strong antioxidant activity, which extended the shelf life of flaxseed oil emulsions. The composition of the PP-TA complexes impacted the aggregation state of the lipid droplets under simulated gastric conditions, which affected the rate and extent of lipid digestion. This study shows PP-TA complexes can be used for fabricating flaxseed oil delivery systems with enhanced oxidative stability and good digestibility.
Flaxseed oil was microencapsulated employing a wall material matrix of either chickpea (CPI) or lentil protein isolate (LPI) and maltodextrin using a benchtop spray dryer. Effects of emulsion ...formulation (oil, protein and maltodextrin levels) and protein source (CPI vs LPI) on the physicochemical characteristics, oxidative stability, and release properties of the resulting capsules were investigated. Microcapsule formulations containing higher oil levels (20% oil, 20% protein, 60% maltodextrin) were found to have higher surface oil and lower encapsulation efficiencies. Overall, LPI–maltodextrin capsules gave higher flaxseed oil encapsulation efficiencies (∼88.0%) relative to CPI–maltodextrin matrices (∼86.3%). However, both designs were found to provide encapsulated flaxseed oil protection against oxidation over a 25 d room temperature storage study relative to free oil. Overall, ∼37.6% of encapsulated flaxseed oil was released after 2 h under simulated gastric fluid, followed by the release of an additional ∼46.6% over a 3 h period under simulated intestinal fluid conditions.
•Thermoplastic starch offers partial miscibility with PLA as evidenced by FESEM.•The addition of 4–6phr MLO leads to an elongation at break of 140–160%.•MLO also provides a compatibilization effect.
...In this work, poly(lactic acid), PLA and thermoplastic starch, TPS blends (with a fixed content of 30wt.% TPS) were prepared by melt extrusion process to increase the low ductile properties of PLA. The TPS used contains an aliphatic/aromatic biodegradable polyester (AAPE) that provides good resistance to aging and moisture. This blend provides slightly improved ductile properties with an increase in elongation at break of 21.5% but phase separation is observed due to the lack of strong interactions between the two polymers. Small amounts of maleinized linseed oil (MLO) can positively contribute to improve the ductile properties of these blends by a combined plasticizing-compatibilizing effect. The elongation at break increases over 160% with the only addition of 6phr MLO. One of the evidence of the plasticizing-compatibilizing effect provided by MLO is the change in the glass transition temperature (Tg) with a decrease of about 10°C. Field emission scanning electron microscopy (FESEM) of PLA-TPS blends with varying amounts of maleinized linseed oil also suggests an increase in compatibility.
Non-isocyanate polyurethanes (NIPU) prepared from renewable resource have attracted much attention and are of great significance for achieving carbon neutrality targets. In this study, a novel ...carbonated linseed oil was synthesized via a coupling reaction between CO2 and epoxidized linseed oil, followed by ring-opening polymerization with two bio-based diamines for preparation of fully renewable NIPU. The effects of different bio-based diamines and different molar ratio of cyclic carbonate groups to amine groups on the thermal-physical, mechanical, and chemical resistance properties of the NIPU films were investigated. These chromophore-free NIPU films demonstrated intense luminescence because of clusteroluminescence induced by the formation of urethane groups with restricted molecular movement. This work reports a simple, environmentally friendly strategy for the development of fully renewable NIPU, which could find potential applications in coatings, elastomers, luminescent materials, and other industries.
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•A series of fully renewable non-isocyanate polyurethanes was successfully prepared.•The mechanical properties of non-isocyanate polyurethanes could be tailored.•Fully renewable non-isocyanate polyurethanes showed intense clusteroluminescence.
Previous works of the authors have presented the changes in the optical, mechanical, and chemical properties of the oiled areas of the supports that occur upon ageing due to oil-binder absorption in ...works of art on paper and printed material. In this framework, transmittance FTIR analysis has indicated that the presence of linseed oil induces the conditions to promote the deterioration of the oil-impregnated areas of the paper supports. However, the analysis of oil-impregnated mock-ups did not provide detailed information about the input of linseed oil formulations and the different types of paper support on the chemical changes that occur upon ageing. This work presents the results of ATR-FTIR and reflectance FTIR, which were used for compensating the previous results, proving indications on the effect of different materials (linseed oil formulations, and cellulosic and lignocellulosic papers) on the development of chemical changes, thus, on the condition of the oiled areas upon ageing. Although linseed oil formulations have a determining effect on the condition of the oiled areas of the support, the paper pulp content appears to have an input to the chemical changes that occur in the system of paper-linseed oil upon ageing. The results presented are more focused on the oil-impregnated mock-ups with cold-pressed linseed oil since results have indicated that this causes more extended changes upon ageing.
•Encapsulation efficiency (EE) of flaxseed oil is 97%.•Pectin microbeads contain up to 76% flaxseed oil (payload).•Fluid bed drying leads to the formation of much fewer hydroperoxides than air ...drying.•Encapsulated oil is 13-fold more stable than bulk oil.
Flaxseed oil is a major source of omega-3 polyunsaturated fatty acids (PUFAs), as it contains nearly 50% of alpha-linolenic acid. For this reason it is highly susceptible to auto-oxidation. The aim of the work was to increase the stability of flaxseed oil by a microencapsulation process based on ionic gelation through vibrating-nozzle extrusion technology, using pectin as shell material. Two different drying systems, passive air drying (AD) and fluid bed (FB), were compared. The results show that the encapsulation efficiency is very high (up to 98%). Besides being approximately 20-fold faster, FB gives beads showing on average higher payload (76% vs 68%) and lower peroxide value (9.64 vs 21.33) than the AD. An accelerated test carried out on FB-dried beads shows that the oxidative stability of encapsulated oil is 13-fold higher than bulk oil (PV FB: 20 vs PV oil: 260), demonstrating the protecting effect of microencapsulation.
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