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•Signatures of the fluorescence spectra of the meat contaminated with Salmonella were measured.•It was observed an increase in NAD and FAD oxidation products fluorescence intensity in ...contaminated meat,•The presence of coproporphyrin fluorescence was observed in contaminated meat.•The results confirmed that fluorescence could be considered as a method to identify biological contamination in meat.
Contaminated poultry products as eggs and meat are the primary vehicles of Salmonella infection. Conventional methods for microorganisms detections involve multiple steps, and despite its accuracy, these assays are time‐consuming. Biosensing methods have shown great potential for the rapid detection of foodborne pathogens. Some of the biosensors are based on fluorescence. Various fluorophores such as collagen, elastin, NAD(P)H, and porphyrins can be used to evaluate possible chemical changes in meat. In this manuscript, the fluorescence properties of chicken meat contaminated with Salmonella enterica (ATCC 14028) cell suspensions (500; 5000; 50,000 and 500,000 cells/mL) were obtained and compared with non-contaminated control, for meat kept at 25 °C for 24 and 48 h. The effects of ambient light were also considered. Our results indicated that free NAD(P)H and coproporphyrin emission bands present in contaminated meat, increased over time, and can provide access to valuable information for the detection of Salmonella in chicken meat.
To identify the microbial community and origin of the spoilage flora of bacon, the changes in microbial population numbers and community structure were followed along the processing line, using ...culture-independent and culture-dependent methods. 16S rRNA gene amplicon sequencing (16S-seq) analysis showed that community complexity and structure significantly differed at different processing stages. Some 428 bacterial groups were ascertained at genus level, and Acinetobacter, Pseudomonas, Psychrobacter, and Brochothrix were the predominant bacteria on raw meats. After curing specimens dominated by Psychrobacter, Weissella, Vibrio, Leuconostoc, Myroides, Acinetobacter, and Lactobacillus, a total of 33 species were identified by traditional microbiological analyses and direct sequence determination methods. Our results indicated that curing should be considered one of the primary factors during various processing steps, presumably contaminating the products directly or indirectly.
•Both traditional cultivation methods and high-throughput sequencing were applied.•The load of microorgansims on raw meat and curing were higher.•Psychrobacter, Weissella, Vibrio, Leuconostoc and Lactobacillus were the predominant species since curing.•Curing should be considered one of the primary factors during the various processing steps.
The objective of this study was to analyze and determine the economic loss from the main causes of whole bovine carcass condemnation in slaughterhouses that are inspected by the Federal Inspection ...Service in the state of São Paulo for the period from 2010 to 2019. Economic loss was calculated from multiplication of the number of whole carcasses condemned by the mean yield of meat per carcass and the mean annual price of beef. The monetary values were updated to the year 2019, using the IGP-DI General Price Index. The results indicated an economic loss of R$ 4.06 billion from the whole condemnation of bovine carcasses and the main causes were contamination (R$ 1.73 billion), abscess (R$ 283.20 million), urinary cyst (R$ 194.14 million), emphysema (R$ 107.00 million) and nephritis (R$ 107.52 million). The main factors associated with the whole condemnation of bovine carcasses are failures in the pre-slaughter management and in the slaughter stages, as well as nutritional disorders. Consequently, to minimize such losses in beef production in São Paulo state it is recommended to adopt good production practices and train slaughterhouse employees.
Introduction: Escherichia coli O157:H7, as a pathogenic agent, can be transmitted through the foods including meat, meat products, dairy products, vegetables and water. The World Health Organization ...has recommended that all countries in the world, especially developing countries, should consider the investigation of E. coli O157:H7 as a research priority. The aim of this study was to determine the frequency of E. coli O157:H7 in meat of cow, sheep, goat, and camel in Kerman province of Iran using culture and polymerase chain reaction (PCR) methods. Methods: In this study, 280 meat samples consisting of sheep (90 specimens), cow (80 specimens), goat (60 specimens) and camel (50 specimens) meats were randomly separated from carcasses from April to July 2018. After the sampling, microbial culture was performed on the samples. Then, suspected E. coli O157:H7 colonies were evaluated by PCR assay. Results: Out of the 280 samples, 73 samples (26%) were contaminated with E. coli. based on bacteriological tests, and 28 samples were identified as suspected E. coli O157:H7 serotype based on the lack of sorbitol fermentation. Subsequently, sorbitol-negative samples were tested by PCR procedure using specific primers. The results revealed that out of 28 cases, 21 cases (7.5%) were E. coli O157:H7. Conclusion: As can be deduced from the observations of this study, to detect the E. coli O157, PCR as an accurate, fast, and reliable procedure can be used along with the culture method.
Biofilms are surface-attached microbial communities with distinct properties, which have a tremendous impact on public health and food safety. In the meat industry, biofilms remain a serious concern ...because many foodborne pathogens can form biofilms in areas at meat plants that are difficult to sanitize properly, and biofilm cells are more tolerant to sanitization than their planktonic counterparts. Furthermore, nearly all biofilms in commercial environments consist of multiple species of microorganisms, and the complex interactions within the community significantly influence the architecture, activity, and sanitizer tolerance of the biofilm society. This review focuses on the effect of microbial coexistence on mixed biofilm formation with foodborne pathogens of major concern in the fresh meat industry and their resultant sanitizer tolerance. The factors that would affect biofilm cell transfer from contact surfaces to meat products, one of the most common transmission routes that could lead to product contamination, are discussed as well. Available results from recent studies relevant to the meat industry, implying the potential role of bacterial persistence and biofilm formation in meat contamination, are reviewed in response to the pressing need to understand the mechanisms that cause "high event period" contamination at commercial meat processing plants. A better understanding of these events would help the industry to enhance strategies to prevent contamination and improve meat safety.
This study addresses the improvement of meat microbial quality by enriching the diet of farm animals with a protective culture. Weaned Grimaud rabbits were divided into two experimental groups: a ...control and a diet supplemented with Micocin® (Carnobacterium maltaromaticum CB1; 8Log10CFU/kg of feed). Overall, meat quality was not affected substantially by the treatment. Total Aerobic Mesophilic (TAM), Escherichia coli and other coliforms, Enterobacteriaceae, Staphylococcus aureus, Pseudomonas spp., Listeria spp. and presumptive lactic acid bacteria counts were evaluated on whole thighs stored under aerobic (0, 3, 6, 8days) and anaerobic (0, 5, 10, 15, 20days) conditions at 4°C. The results demonstrated that the microflora on refrigerated thighs was modulated by the addition of Micocin® (P<0.05) and that the most effective reduction of Listeria monocytogenes growth was observed with ground meat stored under anaerobic conditions at 4°C with a 2 Log difference at the end of a 15-day storage (P=0.025).
The presence of some drugs in meat samples can cause threat to human health, therefore, its analysis is highly desirable for food safety purposes. In this work, a solid-phase extraction procedure for ...the determination of oxprenolol, a non-selective beta-blocker, and such anabolic agents as methandienone and testosterone in beef meat samples has been developed. Extraction conditions were optimized to achieve high sensitivity and accuracy of the results. The procedure was validated using meat samples free from target analytes. As a result, high selectivity and sensitivity were observed with the detection limits between 0.25 and 1.25 ng/g, and the results were not affected by matrix components. The proposed procedure was applied to the analysis of real beef samples purchased in the market, and the results have revealed the presence of contaminated samples. The concentration of oxprenolol in the contaminated sample was 7 ng/g, methandienone content in the sample was 30 ng/g, while testosterone level was 4 ng/g.
•A procedure is proposed for the determination of oxprenolol, methandienone and testosterone in meat samples.•Solid-phase extraction was used for beef sample preparation.•LC-HRMS was used to detect oxprenolol, methandienone and testosterone with high accuracy.•The analysis of 20 real beef samples was conducted, three contaminated samples were found.
Clenbuterol is a β2 agonist agent with anabolic properties given by the increase in the muscular mass in parallel to the decrease of the body fat. For this reason, the use of clenbuterol is forbidden ...by the World Anti-Doping Agency (WADA) in the practice of sport. This compound is of particular interest for anti-doping authorities and WADA-accredited laboratories due to the recent reporting of risk of unintentional doping following the eating of meat contaminated with traces of clenbuterol in some countries. In this work, the development and the validation of an ultra-high pressure liquid chromatography coupled to electrospray ionization tandem mass spectrometry (UHPLC–ESI-MS/MS) method for the quantification of clenbuterol in human urine is described. The analyte was extracted from urine samples by liquid–liquid extraction (LLE) in basic conditions using tert butyl-methyl ether (TBME) and analyzed by UHPLC–MS/MS with a linear gradient of acetonitrile in 9min only. The simple and rapid method presented here was validated in compliance with authority guidelines and showed a limit of quantification at 5pg/mL and a linearity range from 5pg/mL to 300pg/mL. Good trueness (85.8–105%), repeatability (5.7–10.6% RSD) and intermediate precision (5.9–14.9% RSD) results were obtained. The method was then applied to real samples from eighteen volunteers collecting urines after single oral doses administration (1, 5 and 10μg) of clenbuterol-enriched yogurts.
Ascarid Larva Migrans Syndrome (ascarid LMS) is a clinical syndrome in humans, caused by the migration of animal roundworm larvae such as Toxocara canis, Toxocara cati and Ascaris suum. Humans may ...acquire infection by ingesting embryonated eggs, or infective larvae of these parasites in contaminated meat and organ meats. To detect these pathogenic contaminations, a novel nested multiplex PCR system was developed. Our novel nested multiplex PCR assay showed specific amplification of T. canis, T. cati and Ascaris spp. Detection limit of the nested multiplex PCR was tested with serial dilution of T. canis, T. cati or A. suum genomic DNA (gDNA) from 100 pg to 100 ag and found to be 10 fg, 1 fg and 100 fg, respectively. When larvae were spiked into chicken liver tissue, DNA of T. canis and A. suum was detected from the liver spiked with a single larva, while the assay required at least 2 larvae of T. cati. Moreover, the ascarid DNA was detected from the liver of mice infected with 100 and 300 eggs of T. canis, T. cati or A. suum. This nested multiplex PCR assay could be useful for the detection of contamination with ascarid larvae in meat and organ meats.
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•A nested multiplex PCR system for the molecular detection of T. canis, T. cati and A. suum was established.•The assay showed highly sensitive amplification of ascarid gDNA and could distinguish these three species in one reaction.•The system could detect ascarid gDNA from experimentally infected mouse livers.
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