The incidence of primary cutaneous melanoma continues to increase each year. Melanoma accounts for the majority of skin cancer–related deaths, but treatment is usually curative following early ...detection of disease. In this American Academy of Dermatology clinical practice guideline, updated treatment recommendations are provided for patients with primary cutaneous melanoma (American Joint Committee on Cancer stages 0-IIC and pathologic stage III by virtue of a positive sentinel lymph node biopsy). Biopsy techniques for a lesion that is clinically suggestive of melanoma are reviewed, as are recommendations for the histopathologic interpretation of cutaneous melanoma. The use of laboratory, molecular, and imaging tests is examined in the initial work-up of patients with newly diagnosed melanoma and for follow-up of asymptomatic patients. With regard to treatment of primary cutaneous melanoma, recommendations for surgical margins and the concepts of staged excision (including Mohs micrographic surgery) and nonsurgical treatments for melanoma in situ, lentigo maligna type (including topical imiquimod and radiation therapy), are updated. The role of sentinel lymph node biopsy as a staging technique for cutaneous melanoma is described, with recommendations for its use in clinical practice. Finally, current data regarding pregnancy and melanoma, genetic testing for familial melanoma, and management of dermatologic toxicities related to novel targeted agents and immunotherapies for patients with advanced disease are summarized.
This paper review is aiming to comprehensively identify and appraise the current available knowledge on microbial composition and microbial dynamics in anaerobic digestion with focus on the ...interconnections between operational parameters and microbial community. We systematically searched Scopus, Web of Science, pubmed and Embase (up to August 2019) with relative keywords to identify English-language studies published in peer-reviewed journals. The data and information on anaerobic reactor configurations, operational parameters such as pretreatment methods, temperature, trace elements, ammonia, organic loading rate, and feedstock composition and their association with the microbial community and microbial dynamics were extracted from eligible articles. Of 306 potential articles, 112 studies met the present review objectives and inclusion criteria. The results indicated that both aceticlastic and hydrogenotrophic methanogenesis are dominant in anaerobic digesters and their relative composition is depending on environmental conditions. However, hydrogenotrophic methanogens are more often observed in extreme conditions due to their higher robustness compared to aceticlastic methangoens. Firmicutes and Bacteroidetes phyla are most common fermentative bacteria of the acidogenic phase. These bacteria secrete lytic enzymes to degrade organic matters and are able to survive in extreme conditions and environments due to their spores. In addition, among archaea Methanosaeta, Methanobacterium, and Methanosarcinaceae are found at high relative abundance in anaerobic digesters operated with different operational parameters. Overall, understanding the shifts in microbial composition and diversity as results of operational parameters variation in anaerobic digestion process would improve the stability and process performance.
•A comprehensive review was performed on microbial composition in anaerobic digestion process.•The environmental and operational parameters extensively influence the microbial community and diversity.•Hydrogenotrophic methanogens are more often observed in extreme conditions due to their higher robustness.•Understanding the shifts in microbial composition and diversity would improve the stability and process performance.
Vibrio parahaemolyticus is a Gram-negative halophilic bacterium that is found in estuarine, marine and coastal environments. V. parahaemolyticus is the leading causal agent of human acute ...gastroenteritis following the consumption of raw, undercooked, or mishandled marine products. In rare cases, V. parahaemolyticus causes wound infection, ear infection or septicaemia in individuals with pre-existing medical conditions. V. parahaemolyticus has two hemolysins virulence factors that are thermostable direct hemolysin (tdh)-a pore-forming protein that contributes to the invasiveness of the bacterium in humans, and TDH-related hemolysin (trh), which plays a similar role as tdh in the disease pathogenesis. In addition, the bacterium is also encodes for adhesions and type III secretion systems (T3SS1 and T3SS2) to ensure its survival in the environment. This review aims at discussing the V. parahaemolyticus growth and characteristics, pathogenesis, prevalence and advances in molecular identification techniques.
Recent advances in the diagnosis of dermatophytosis Begum, Jubeda; Mir, Nasir A.; Lingaraju, Madhu C. ...
Journal of basic microbiology,
April 2020, 2020-Apr, 2020-04-00, Volume:
60, Issue:
4
Journal Article
Peer reviewed
Dermatophytosis is a disease of global significance caused by pathogenic keratinolytic fungi called dermatophytes in both animals and humans. The recent taxonomy of dermatophytes classifies them into ...six pathogenic genera, namely Microsporum, Trichophyton, Epidermophyton, Nannizzia, Lophophyton and Arthroderma. It is because of the delayed diagnostic nature and low accuracy of dermatophyte detection by conventional methods that paved the path for the evolution of molecular diagnostic techniques, which provide the accurate and rapid diagnosis of dermatophytosis for an appropriate, timely antifungal therapy that prevents the nonspecific over‐the‐counter self‐medication. This review focuses on the importance of rapid and accurate diagnosis of dermatophytosis, limitations of conventional methods, selection of targets in diagnosis, and factors affecting sensitivity and specificity of various molecular diagnostic technologies in the diagnosis of dermatophytosis. Generally, all the molecular techniques have a significant edge over the conventional methods of culture and microscopy in the dermatophytosis diagnosis. However, in mycology laboratory, the suitability of any molecular diagnostic technique in the diagnosis of dermatophytosis is driven by the requirement of time, economy, complexity, the range of species spectrum detected and the scale of diagnostic output required. Thus, various choices involved in the pursuit of a diagnosis of dermatophytosis are determined by the available conditions and the facilities in the laboratory.
Considering the future energy demand and pollution to the environment, biohydrogen, a biofuel, produced from biological sources have garnered increased attention. The present review emphasis the ...various techniques and methods employed to enumerate the microbial community and enhancement of hydrogen production by dark fermentation. Notably, molecular techniques such as terminal restriction fragment length polymorphism (T-RFLP), quantitative real-time PCR (q-PCR), fluorescent in-situ hybridization (FISH), denaturing gradient gel electrophoresis (DGGE), ribosomal intergenic spacer analysis (RISA), and next generation sequencing (NGS) have been extensively discussed on identifying the microbial population in hydrogen production. Further, challenges and merits of the molecular techniques have been elaborated.
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•Molecular techniques for analyzing biohydrogen-producers were addressed.•T-RFLP, RT-PCR, FISH, DGGE and NGS have been extensively discussed.•Primers and FISH probes for target strain identification were given.•Advantages and challenges of the molecular tools have been elaborated.
Spices have great monetary value, and it has a vast range of functionalities such as flavouring of food, perfumery, cosmetics, medicinal use, and preservative properties Adulterant authentication in ...spices plays a vital role in quality control. The consumers demand increased food quality and safety concerns and food products with high traceability. Food product authenticity has become a significant factor in marking the quality, authenticity, origin of food, etc. It assures buyers, traders, and importing countries that the product is of high quality. Though physical methods are simple to use, the time taken to screen the sample limits commercial application. Analytical methods such as chemical, analytical and instrumental techniques have been widely used. Complex instruments, and data processing restricts its routine use. Molecular techniques can be the powerful tool for detecting adulterants in spices due to its rapidity, sensitivity, accuracy and cost-effectiveness. This review provides an overview of applications, merits & demerits and the challenges of various techniques used for adulterant authentication in spices and herbs.
Chlamydiae have a worldwide distribution causing a wide range of diseases in human hosts, livestock, and companion animals as well as in wildlife and exotic species. Moreover, they can persist in ...their hosts as asymptomatic infections for extended periods of time. The introduction of molecular techniques has revolutionized the Chlamydia field by expanding the host range of known chlamydial species but also by discovering new species and even new families of bacteria in the broader order Chlamydiales. The wide range of hosts, diseases, and tissues affected by chlamydiae complicate the diagnosis such that standard diagnostic approaches for these bacteria are rare. Bacteria of the Chlamydiales order are small and their inclusions are difficult to detect by standard microscopy. With the exception of avian and ovine chlamydiosis, macroscopic and/or histologic changes might not be pathognomic or indicative for a chlamydial infection or even not present at all. Moreover, detection of chlamydial DNA in specimens in the absence of other methods or related pathological lesions questions the significance of such findings. The pathogenic potential of the majority of recently identified Chlamydia-related bacteria remains largely unknown and awaits investigation through experimental or natural infection models including histomorphological characterization of associated lesions. This review aims to summarize the historical background and the most important developments in the field of animal chlamydial research in the past 5 years with a special focus on pathology. It will summarize the current nomenclature, present critical thoughts about diagnostics, and give an update on chlamydial infections in domesticated animals such as livestock, companion animals and birds, as well as free-ranging and captive wild animals such as reptiles, fish, and marsupials.
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