Examines the extent in variation in the properties of a) variation in the population size and b) the effectiveness of N-fixation at three differentspatial scales namely: from 26 sites across New ...Zealand; at farm-wide scale; and within single fields. Source: National Library of New Zealand Te Puna Matauranga o Aotearoa, licensed by the Department of Internal Affairs for re-use under the Creative Commons Attribution 3.0 New Zealand Licence.
S-adenosylmethionine decarboxylase (SAMDC) mediates the biosynthesis of polyamines (PAs) and plays a positive role in plants’ response to adversity stress tolerance. In this study, we isolated a ...SAMDC gene from white clover, which is located in mitochondria. It was strongly induced when white clover exposed to drought (15% PEG6000), salinity (200 mM NaCl), 20 μM spermidine, 100 μM abscisic acid, and 10 mM H2O2, especially in leaves. The INVSc1 yeast introduced with TrSAMDC1 had tolerance to drought, salt, and oxidative stress. Overexpression of TrSAMDC1 in Arabidopsis showed higher fresh weight and dry weight under drought and salt treatment and without growth inhibition under normal conditions. Leaf senescence induced by drought and saline was further delayed in transgenic plants, regardless of cultivation in 1/2 MS medium and soil. During drought and salt stress, transgenic plants exhibited a significant increase in relative water content, maximum photosynthesis efficiency (Fv/Fm), performance index on the absorption basis (PIABS), activities of antioxidant protective enzymes such as SOD, POD, CAT, and APX, and a significant decrease in accumulation of MDA and H2O2 as compared to the WT. The concentrations of total PAs, putrescine, spermidine, and spermidine in transgenic lines were higher in transgenic plants than in WT under normal and drought conditions. These results suggested that TrSAMDC1 could effectively mitigate abiotic stresses without the expense of production and be a potential candidate gene for improving the drought and salt resistance of crops.
•TrSAMDC1 located in mitochondria positively respond to drought, salt, Spm, ABA, and H2O2 treatments in white clover.•TrSAMDC1 conferred drought, salt and oxidative tolerance in transgenic yeasts.•Overexpression of TrSAMDC1 enhanced drought and salt resistance in Arabidopsis.•Overexpression of TrSAMDC1 improved endogenous PAs titers and antioxidant capacities.
Phosphorus (P) loss from highly fertilised grazed pastures can impair surface water quality. High P concentrations in grazed pastures are maintained to boost legume production, but systems that use ...monocultures of grass and clover can be more profitable and decrease P losses by lowering soil P in grassed areas. In contrast with using a direct drill to establish new pastures, conventional cultivation can lower topsoil P quickly and can be used as part of a farm re-grassing programme every six years. We tested if the potential for low P losses (measured by water extractable P; WEP) could be maintained over six years in pastures (ryegrass and white clover monocultures and a ryegrass and clover mixed sward) despite being fertilised (10, 35 and 100 kg P ha-1) and grazed. Cultivation (0–20 cm) decreased WEP and Olsen P in all three pastures and P rates by 30–50 % compared to direct drill and maintained this decrease for six years. A threshold in Olsen P (22 mg L-1) was noted where WEP concentrations increased at a greater rate relative to Olsen P than below. This threshold was like the critical point in Olsen P for 97 % relative yield in the pastures (22–28 mg L-1) and could be used to advise on cultivation and P fertiliser strategies to help avoid the potential for P loss without significantly compromising yield. The data indicated that cultivation could be used to quickly establish and maintain lower soil P concentrations as part of a strategy to decrease P losses and improve profit by using a split grass-clover system.
•Phosphorus runoff from land can impair surface water quality.•Splitting pastures into monocultures maintains production and enables low P plants to grow in runoff prone areas.•Conventional cultivation can be used to quickly decrease enriched soil P for low P plants.•Potential for runoff was maintained despite fertiliser P and grazing returns over 6-year period.•Conventional cultivation is a suitable method for re-grassing programme to lower runoff P losses.
White clover (Trifolium repens L.; clover) can offer a superior nutritional feed compared with perennial ryegrass (Lolium perenne L.; PRG) and offers an additional or alternative source (or both) of ...N for herbage production. The objective of this study was to investigate the effect of including clover into PRG swards receiving 150 (Cl150) or 250 kg of N/ha (Cl250) compared with a PRG-only sward receiving 250 kg of N/ha (Gr250) on herbage production, milk production, and herbage dry matter intake (DMI) in an intensive grass-based spring calving milk production system over 2 full lactations. A farm systems experiment was established in February 2013, and conducted over 2 grazing seasons 2013 (yr 1) and 2014 (yr 2). In February 2013 (yr 1), 42 Holstein-Friesian spring-calving dairy cows, and in February 2014 (yr 2), 57 Holstein-Friesian spring-calving dairy cows were allocated to graze the Cl150, Cl250, and Gr250 swards (n = 14 in yr 1 and n = 19 in yr 2) from February to November, at a stocking rate of 2.74 cows/ha. Herbage DMI was estimated twice in yr 1 (May and September) and 3 times in yr 2 (May, July, and September). Treatment did not have a significant effect on annual herbage production. Sward clover content was greater on the Cl150 treatment than the Cl250 treatment. The cows grazing both clover treatments (Cl250 and Cl150) produced more milk than the cows grazing Gr250 from June until the end of the grazing season. A significant treatment by measurement period interaction was observed on total DMI. In May, the cows on the Cl250 treatment had the greatest DMI. In July, the cows on the clover treatments had greater DMI than those on the Gr250 treatment, whereas in September, the cows on the Cl150 treatment had the lowest DMI. In conclusion, including clover in a PRG sward grazed by spring-calving dairy cows can result in increased animal performance, particularly in the second half of lactation. Reducing N fertilizer application to 150 kg of N/ha on grass-clover swards did not reduce herbage production compared with grass-only swards receiving 250 kg of N/ha. White clover can play an integral role in intensive grazing systems in terms of animal performance and herbage production.
In dairy systems, grass–clover swards are fertilized with on–farm cattle slurry as a basic dressing and often supplemented with mineral fertilizer throughout the season. Uncertainty remains on ...consequences for N input from atmospheric N2-fixation by grassland legumes. The aim of this study was to examine the N response of slurry versus mineral N fertilization on clover dynamics, the proportion of N derived from the atmosphere (%Ndfa) and the quantitative N2-fixation (qBNF) using the 15N isotope dilution method. This was done in an on farm setting at two sites in two years with increasing rates of N fertilizer (0–480 kg available N ha-1) either of mineral N only or combined with application of cattle slurry. The leys were at one site composed a 2–species mixture of white clover (Trifolium repens L.) and ryegrass (Lolium perenne L.) and at the other site of a 4–species mixture of white clover, red clover (Trifolium pretense L.), festulolium (Festulolium braunii) and ryegrass. Species dynamics were significantly affected by fertilizer rate in the 2–species sward and by fertilizer rate and type in the 4–species sward. Reduction of the clover proportion in response to fertilization was due to a clover yield decrease (2–species sward) or a grass yield increase (4–species sward). In the 4–species sward, in treatments with mineral N only %Ndfa decreased markedly with N rate, whereas the corresponding slurry + mineral N treatments did not. Thus, apart from fertilization with mineral N only at one site the general picture was a relatively high (>80%) %Ndfa over the season when the N level was below 200–300 kg available N ha-1. The qBNF of the harvested biomass in sward without fertilization reached 193–216 kg N ha-1 in the 2–species sward and 203–286 kg N ha-1 in the 4–species sward. Clover persisted at high fertilization rates, but qBNF was reduced to 16–79 kg N ha-1 and 58–163 kg N ha-1 in 2– and 4–species swards, respectively. Interestingly, in the 4–species sward amended with slurry + mineral N, qBNF was high and independent of fertilization rate. We conclude that response of clover dynamics to N fertilization differed with fertilizer type and level – slurry have lower effect whereas the effect of mineral N only on N2-fixation is less predictable.
•N response of grass–clover leys was investigated as affected by mineral N or slurry.•Dry matter yield and grass fraction increased as expected with N level.•Leys at two sites showed two patterns of N2-fixation activity response to fertilizer type.•Slurry in combination with mineral N sustained high N2-fixation until 240 kg N ha-1.•Clover N2-fixation response to mineral N only was less predictable among sites.
Grazed pastures are a major contributor to emissions of the greenhouse gas nitrous oxide (N2O), and urine deposition from grazing animals is the main source of the emissions. Incorporating ...alternative forages into grazing systems could be an approach for reducing N2O emissions through mechanisms such as release of biological nitrification inhibitors from roots and increased root depth. Field plot and lysimeter (intact soil column) trials were conducted in a free draining Horotiu silt loam soil to test whether two alternative forage species, plantain (Plantago lanceolate L.) and lucerne (Medicago sativa L.), could reduce N2O emissions relative to traditional pasture species, white clover (Trifolium repens L.) and perennial ryegrass (Lolium perenne L.). The amounts of N2O emitted from the soil below each forage species, which all received the same cow urine at the same rates, was measured using an established static chamber method. Total N2O emissions from the plantain, lucerne and perennial ryegrass controls (without urine application) were generally very low, but emissions from the white clover control were significantly higher. When urine was applied in autumn or winter N2O emissions from plantain were lower compared with those from perennial ryegrass or white clover, but this difference was not found when urine was applied in summer. Lucerne had lower emissions in winter but not in other seasons. Incorporation of plantain into grazed pasture could be an approach to reduce N2O emissions. However, further work is required to understand the mechanisms for the reduced emissions and the effects of environmental conditions in different seasons.
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•Urine patches in ryegrass-dominant pasture are a major contributor to N2O emissions.•Test of alternative pasture species as a novel approach to reduce N2O emissions•Compared with ryegrass, plantain had lower emissions from urine in most seasons.•Lucerne had lower emissions in winter but not in other seasons.
Urbanization transforms environments in ways that alter biological evolution. We examined whether urban environmental change drives parallel evolution by sampling 110,019 white clover plants from ...6169 populations in 160 cities globally. Plants were assayed for a Mendelian antiherbivore defense that also affects tolerance to abiotic stressors. Urban-rural gradients were associated with the evolution of clines in defense in 47% of cities throughout the world. Variation in the strength of clines was explained by environmental changes in drought stress and vegetation cover that varied among cities. Sequencing 2074 genomes from 26 cities revealed that the evolution of urban-rural clines was best explained by adaptive evolution, but the degree of parallel adaptation varied among cities. Our results demonstrate that urbanization leads to adaptation at a global scale.
The objective of this study was to examine the effects of arbuscular mycorrhizal fungi (AMF) and organic manure on the growth and element uptake of Trifolium repens and the soil chemical and ...biological characteristics in sterilized and natural soils. An increase in organic manure input reduced mycorrhizal colonization in the sterilized soil treatment. In comparison to the treatments in which AMF inoculation did not occur, AMF inoculation significantly promoted the biomass of shoots and roots in the sterilized soil at the 2.5 and 5% organic manure incorporation levels, whereas AMF had no effect on biomass in the natural soil. Regardless of soil type and whether AMF inoculation occurred, the N, P, K and Mg concentrations of the shoots and roots and the Cd concentration of the roots were increased by organic manure amendment. In comparison to the 2.5% organic manure amendment treatment, the addition of 5% organic manure to the sterilized soil decreased the N, P, K, Ca and Cd contents in the absence of AMF but had no negative effect on the N, P, K, Mg and Cd contents in the presence of AMF. The soil available P, pH, microbial biomass carbon, microbial biomass nitrogen, dissolved organic carbon, diethylenetriamine pentaacetic acid (DTPA)-extractable Cd and root Cd concentrations increased with increasing organic manure input. These results indicate that additive effects of AMF inoculation and organic manure on the growth and nutrient contents of Trifolium repens occurred in the Cd-contaminated sterilized soil but not in the natural soil, implying the limited function of nonnative AMF introduced into natural soils.
White clover (WC) offers an alternative source of nitrogen (N) for pasture-based systems. Substituting energy- and carbon-intensive synthetic N fertilizers with N derived from biological fixation by ...WC has been highlighted as a promising environmental mitigation strategy through the omission of emissions, pollutants, and energy usage during the production and application of synthetic fertilizer. Therefore, the objective was to investigate the effect of the inclusion of WC in perennial ryegrass (PRG) swards on the environmental impact of pasture-based dairy systems. Cradle-to-farm gate life cycle assessment of 3 pasture-based dairy systems were conducted: (1) a PRG–WC sward receiving 150 kg of N/ha per year (CL150), (2) a PRG–WC sward receiving 250 kg of N/ha per year (CL250), and (3) a PRG-only sward receiving 250 kg of N/ha per year (GR250). A dairy environmental model was updated with country-specific N excretion equations and recently developed N2O, NH3, and NO3− emission factors. The environmental impact categories assessed were global warming potential, nonrenewable energy, acidification potential, and eutrophication potential (marine and freshwater). Impact categories were expressed using 2 functional units: per hectare and per metric tonne of fat- and protein-corrected milk. The GR250 system had the lowest milk production and highest global warming potential, nonrenewable energy, and acidification potential per tonne of fat- and protein-corrected milk for all systems. The CL250 system produced the most milk and had the highest environmental impact across all categories when expressed on an area basis. It also had the highest marine eutrophication potential for both functional units. The impact category freshwater eutrophication potential did not differ across the 3 systems. The CL150 system had the lowest environmental impact across all categories and functional units. This life cycle assessment study demonstrates that the substitution of synthetic N fertilizer with atmospheric N fixed by WC has potential to reduce the environmental impact of intensive pasture-based dairy systems in temperate regions, not only through improvement in animal performance but also through the reduction in total emissions and pollutants contributing to the environmental indicators assessed.
Soil salinity is an increasingly serious problem worldwide that reduces agricultural output potential. Selected beneficial soil bacteria can promote plant growth and augment tolerance to biotic and ...abiotic stresses. Bacillus subtilis strain GB03 has been shown to confer growth promotion and abiotic stress tolerance in the model plant Arabidopsis thaliana. Here we examined the effect of this beneficial soil bacterium on salt tolerance in the legume forage crop, white clover. Plants of white clover (Trifolium repens L. cultivar Huia) were grown from seeds with or without soil inoculation of the beneficial soil bacterium Bacillus subtilis GB03 supplemented with 0, 50, 100, or 150 mM NaCl water into soil. Growth parameters, chlorophyll content, malondialdehyde (MDA) content and osmotic potential were monitored during the growth cycle. Endogenous Na(+) and K(+) contents were determined at the time of harvest. White clover plants grown in GB03-inoculated soil were significantly larger than non-inoculated controls with respect to shoot height, root length, plant biomass, leaf area and chlorophyll content; leaf MDA content under saline condition and leaf osmotic potential under severe salinity condition (150 mM NaCl) were significantly decreased. Furthermore, GB03 significantly decreased shoot and root Na(+) accumulation and thereby improved K(+)/Na(+) ratio when GB03-inoculated plants were grown under elevated salt conditions. The results indicate that soil inoculation with GB03 promotes white clover growth under both non-saline and saline conditions by directly or indirectly regulating plant chlorophyll content, leaf osmotic potential, cell membrane integrity and ion accumulation.
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