Gozdni klop (Ixodes ricinus) kot prenašalec babezij v Sloveniji : magistrska naloga
Duh, Darja
Intraerythrocytic parasites of the genus Babesia are tick-transmitted protozoal microorganisms. Babesial parasites require both a competent vertebrate host and nonvertebrate vector to maintain ...transmission cycle. All babesiae described to date are transmitted by ixodid ticks. Babesiosis, causedby babesiae, is a well-known disease of veterinary and economic importance of cattle, horses and dogs. However, it is gaining increasing attention as an emerging zoonotic disease in humans. Three distinctly different babesial parasites, Babesia divergens, Babesia microti and WA-1 typecause human babesiosis. European sheep tick (Ixodes ricinus), most widely distributed tick in Europe and in Slovenia, is the probable vector of babesialparasites in Europe. Moreover, Ixodes ricinus transmits variety of pathogenic microorganisms including viruses and bacteria. No research has beendone yet to determine the incidence and species of babesial parasites in Slovenia. Therefore we set up this study to examine whether Ixodes ricinus ticks, collected in five different zoogeographical regions of Slovenia, are infected with babesial parasites. The species of detected parasites were assessed by using molecular methods . In the year 1997 and 1998, 858 Ixodes ricinus ticks were collected by flagging vegetation in all five zoogeographical regions of Slovenia. The mitochondrial gene of 16S rRNA of tick origin was successfully amplified in 98.8% of tested ticks. Different babesial parasites were detected in ticks by molecular methods, PCR and subsequent sequence analysis. Babseial gene for 18S rRNA was amplified with PIRO A and PIRO B primers in nine adult ticks (4,3%) and nineteen groups of nymphs, calculated incidence of infection of nymphs was 4.0%. (Abstract truncated at 2000 characters).
Type of material - master's thesis
Publication and manufacture - Ljubljana : [D. Duh], 2002
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