Ugotavljanje podtipov virusa Epstein-Barr v naši populaciji : diplomska naloga = Identification of Epstein-Barr subtypes in our population : graduation thesis
Klemenc, Polona
Analize nukleotidnega zaporedja so pokazale, da obstaja precejšna variabilnost med različnimi izolati virusa Epstein - Barr (VEB). Glede na stopnjo različnosti nukleotidnega zaporedja uvrščamo ...izolate VEB v dva tipa (tip A in B) in v številne podtipe. Opredelitev genotipa VEB omogočajo metode molekularne genotipizacije. V naši raziskavi smo skušali v različnih kliničnih materialih bolnikov z različnimi kliničnimi slikami določiti tip VEB.Pri tem smo uporabljali molekularno metodo PCR. Za potrditev specifičnosti rezultatov PCR smo uporabljali elektroforezo v agaroznem gelu in tudi DEIA. Pri določanju tipov VEB nismo bili najbolj uspešni, saj smo tip VEB določili le v treh materialih. Tako smo v celični liniji B95 - 8 določili tip A VEB, v dveh vzorcih plazem pa tip B VEB. Očitno je plazma najboljši material za določanje prisotnosti VEB in njegovega tipa, zato bi veljalo raziskavo nadaljevati na večjem številu vzorcev plazem in določiti tip VEB, ki prevladuje v Sloveniji.
Type of material - undergraduate thesis
; adult, serious
Publication and manufacture - Ljubljana : [P.Klemenc] : [BF, Enota medoddelčnega študija mikrobiologije], 2000
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