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  • Novel europium-tetracycline probe for phosphate determination in microtiter plate
    Turel, Matejka ...
    A new luminiscent europium probe is presented for the determination of phosphate in microtiter plate format. The assay is based on the quenching of the luminiscence of the europium-tetracycline 1:1 ... complex by phosphate using a reagent concentration of 20.8 ▫$\mu$▫mol/L. The probe is excited at 400 nm displays large Stokes shift of 210 nm. The emission maximum is located at 616 nm. The system works best at neutral pH 7. The linear range of the calibrationplot is from ▫$5\cdot10^-6$▫ mol/L to ▫$7\cdot10^-4$▫ mol/L of phosphate, and the limit of detection is 5 ▫$\mu$▫ mol/l.
    Type of material - conference contribution
    Publish date - 2006
    Language - english
    COBISS.SI-ID - 10800150