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  • An optical biosensor based on Hi▫$s_6$▫-OPH for organophosphate detection
    Frančič, Nina ...
    In this work we describe a method for immobilizing the hexahistidine-modified OPH (His6-OPH) enzyme via sol-gel technology in a porous material while retaining its catalytic activity. The potential ... use of each bio-sensing material was checked by quantifying the enzymatic properties, such as the relative activity of the immobilized enzyme, its Michaelis- Menten equation parameters and its stability under extreme working conditions (pH, T). The bio-sensor material was also characterised by SEM as well as in terms of its reversibility and sensitivity.
    Type of material - conference contribution
    Publish date - 2011
    Language - english
    COBISS.SI-ID - 15133462
    DOI