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  • Coenzyme regeneration in hexanol oxidation catalyzed by alcohol dehydrogenase
    Vrsalović Presečki, Ana ; Makovšek, Katja ; Vasić-Rački, Đurđa
    The enzymatic ways of coenzyme regeneration include the addition of a second enzyme to the system or the addition of the co-substrate. In the present study, both methods of enzymatic coenzyme (NAD+) ... regeneration were studied andcompared in the reaction of hexanol oxidation catalyzed by alcohol dehydrogenase (ADH). As a source of ADH, commercial isolated enzyme and the whole bakerćs yeast cells were used. First, coenzyme regeneration was employedin the reaction of acetaldehyde reduction catalyzed by the same enzymethat catalyzed the main reaction, and then NAD+ regeneration was appliedin the reaction of pyruvate reduction catalyzed by L-lactate dehydrogenase (L-LDH). Hexanal was obtained as the product of hexanol oxidation catalyzed by isolated ADH while hexaonic acid was detected as a product of the same reaction catalyzed by bakerćs yeast cells. All of the usedbiocatalysts were kinetically characterized. The mass reactions were described by the mathematical models. All models were validated in the batch reactor. One hundred percent hexanol conversion was obtained using permeabilized yeast cells using both methods of cofactor regeneration. By using isolated enzyme ADH, the higher conversion was achieved in a system with cofactor regeneration catalyzed by L-LDH.
    Source: Applied biochemistry and biotechnology. - ISSN 0273-2289 (Vol. 167, no. 3, 2012, str. 595-611)
    Type of material - article, component part
    Publish date - 2012
    Language - english
    COBISS.SI-ID - 16069654
    DOI

source: Applied biochemistry and biotechnology. - ISSN 0273-2289 (Vol. 167, no. 3, 2012, str. 595-611)

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