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  • Assay for optical determination of biogenic amines using microtiterplates
    Nedeljko, Polonca ; Turel, Matejka ; Lobnik, Aleksandra
    Direct determination of catecholamine noradreanaline (NOR) is presented using o-phthaldialdehyde (OPA) as an indicator reagent. The fluorescent assay in which OPA forms with NOR a fluorescent complex ... (OPA-NOR) can be monitored at neutral, physiological conditions (pH 7) and performed in microtiterplates. The determination of NOR is optimal in the concentration range from 4.0*10-7 to 1.0*10-5 M and limit of detection is 4.0*10-7 M. The OPA-NOR complex maximum intensity is reached within 5 minutes. Dopamine and adrenaline could not be determined using the same approach.
    Source: Optical Sensors 2013 : proceedings of SPIE, 15-17 April 2013, Prague, Czech Republic (Str. 87741A-1-87741A-7)
    Type of material - conference contribution
    Publish date - 2013
    Language - english
    COBISS.SI-ID - 17132054
    DOI

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