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Urease adsorption and activity on magnetite nanoparticles functionalized with monofunctional and bifunctional surface layersPogorilyi, Roman P. ...The surface of magnetite nanoparticles was coated with functional polysiloxane layers using reaction of hydrolytic copolycondensation of tetraethoxysilane and 3-aminopropyltriethoxysilane (or ... N-[3-trimethoxysilylpropyl]ethylendiamine), and also that of tetraethoxysilane, 3-aminopropyltriethoxysilane and methyltriethoxysilane (or n-propyltriethoxysilane). It was shown that these functionalized magnetically controllable particles (about 60%150 nm in size as aggregates), as opposed to magnetite, adsorb urease well from aqueous solutions (up to 1 g/g), and that the level of residual activity of adsorbed layers is up to 84 % in the case of a bifunctional sample. It was established that the activity of immobilized urease is normally gradually reduced during storage of the samples, but in the case of ethylenediamine functional group is not decreased for 45 days. The synthesized samples are promising for use as magnetically directed biocatalysts.Source: The Second Conference of CIS Countries "Sol-Gel-2012", Sevastopol, Crimea, Ukraine, September 18-20, 2012 [Elektronski vir] (Vol. 68, iss. 3, Dec. 2013, str. 447-454)Type of material - conference contributionPublish date - 2013Language - englishCOBISS.SI-ID - 17253142
Author
Pogorilyi, Roman P. |
Melnyk, Inna V. |
Zub, Yuriy L. |
Seisenbaeva, Guliam A. |
Kessler, Vadim G. |
Shcherbatyik, Mykola M. |
Košak, Aljoša |
Lobnik, Aleksandra
Topics
ureaza |
adsorpcija |
hidrolitska polikondenzacija |
magnetni nanodelci |
funkcionalizacija |
površinska obdelava |
biokataliza |
encimi |
hydrolytic polycondensation reaction |
magnetite |
aminogroups surface layer |
urease |
adsorption |
activity
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source: The Second Conference of CIS Countries "Sol-Gel-2012", Sevastopol, Crimea, Ukraine, September 18-20, 2012 [Elektronski vir] (Vol. 68, iss. 3, Dec. 2013, str. 447-454)
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Pogorilyi, Roman P. | ![]() |
Melnyk, Inna V. | ![]() |
Zub, Yuriy L. | ![]() |
Seisenbaeva, Guliam A. | ![]() |
Kessler, Vadim G. | ![]() |
Shcherbatyik, Mykola M. | ![]() |
Košak, Aljoša | 22280 |
Lobnik, Aleksandra | 01407 |
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