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The immobilization of β-galactosidase on glass fiber rolls [Elektronski vir]Pečar, Darja ; Zečević, Katja ; Goršek, Andreja, 1961-The usability of glass fibers as immobilization support with a porous open structure was investigated. We developed a method to immobilize the enzyme β-galactosidase on special glass fiber rolls. The ... new method is simple, non-expensive and industrially applicable. Glutaraldehyde was used as a non-specific cross-linking agent for the covalent binding of β-galactosidase on modified glass fibers. The efficiency of immobilization was tested with the known hydrolysis of lactose. All experiments were performed in a continuous laboratory reactor. The influence of the reaction temperature (20, 25 and 30 °C), the substrate flow rate (1, 2 and 3 mL/min) and the pH of the reaction medium (6, 7 and 8) on the conversion was investigated. The reaction efficiency was monitored by measuring the glucose concentration with a spectrophotometer. High immobilization efficiency, enzyme activity and stability were obtained. The optimal reaction temperature, substrate flow rate and pH were found. The activity and stability of the enzyme entrapped on the glass fiber rolls remained almost unchanged during reuse, which is promising for potential industrial applications.Source: Catalysts [Elektronski vir]. - ISSN 2073-4344 (Vol. 13, no. 12, [article no.] 1503, 5 Dec. 2023, 11 str.)Type of material - e-article ; adult, seriousPublish date - 2023Language - englishCOBISS.SI-ID - 176801027
Author
Pečar, Darja |
Zečević, Katja |
Goršek, Andreja, 1961-
Topics
beta galaktozidaza |
imobilizacija |
steklena vlakna |
beta-galaktosidase |
immobilization |
glass fiber |
lactose
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Author | Pečar, Darja ; Zečević, Katja ; Goršek, Andreja, 1961- |
Title | The immobilization of β-galactosidase on glass fiber rolls [Elektronski vir] |
Publication date | 2023-12-09 |
COBISS.SI-ID | 176801027 |
Publication version in repository | Publisher's version |
Publication licence | Creative Commons Attribution 4.0 International |
Embargo | Immediate publication for public |
Project(s) from which the publication was funded
Title | Acronym | Project ID | Funder |
---|---|---|---|
Procesna sistemska tehnika in trajnostni razvoj | P2-0414-2022 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
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Pečar, Darja | 19271 |
Zečević, Katja | ![]() |
Goršek, Andreja, 1961- | 06008 |
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