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Vpliv različnih načinov sterilizacije in tipa brstov na uspešno vzpostavitev tkivne kulture pri navadni bodiki ( Ilex aquifolium L.) [Elektronski vir] : diplomsko deloModrič, UrškaV letu 2023 smo na Fakulteti za kmetijstvo in biosistemske vede v Mariboru opravili poskus, v katerem smo proučevali vpliv različnih načinov sterilizacije in vpliv tipa brstov (apikalni in aksilarni) ... na vzpostavitev tkivne kulture navadne bodike (Ilex aquifolium L.). Za razkuževanje smo uporabili dve različni sterilizacijski sredstvi, dikloroizocianurno kislino (DICA) in natrijev hipoklorit (NaClO), v različnih koncentracijah in z različnim časom tretiranja. V raziskavo je bilo vključenih 640 brstov, na katerih smo skupno opravili 7 različnih načinov sterilizacije. Vse brste smo predhodno tretirali v 70-% etanolu za 40 sekund. Razkužene brste smo prenesli na osnovno gojišče McCown Woody Plant in spremljali rast. Ugotovili smo, da tako način sterilizacije kot tudi tip brstov vplivata na pridobljeno število vitalnih poganjkov. Skupno smo uspešno sterilizirali 22 brstov. Za uspešnejše so se izkazali apikalni brsti. Pri primerjavi sterilizacijskih sredstev se je tretiranje z DICA izkazalo za uspešnejše, pridobili smo 18 vitalnih brstov. Najuspešnejša je bila sterilizacija brstov z DICA in časom tretiranja 5 minut, kjer smo pridobili 7 vitalnih brstovType of material - undergraduate thesis ; adult, seriousPublication and manufacture - Maribor : [U. Modrič], 2023Language - slovenianCOBISS.SI-ID - 179955971
Author
Modrič, Urška
Other authors
Šiško, Metka, 1970- |
Ivanuš, Anja
Topics
Univerzitetna in visokošolska dela |
sterilizacija |
tkivne kulture |
bodika |
in vitro |
brsti |
sterilisation |
tissue culture |
holly |
in vitro |
buds
Shelf entry
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| Year | Impact factor | Edition | Category | Classification | ||||
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| JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP | |
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DRS, in which the journal is indexed
| Database name | Field | Year |
|---|
| Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
|---|---|
| Modrič, Urška |  |
| Šiško, Metka, 1970- | 18684 |
| Ivanuš, Anja | |
Source: Personal bibliographies
and: SICRIS
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