The use of compressed gases as alternative solvents [Elektronski vir]
Knez, Željko ; Leitgeb, Maja
The application of SCF and liquid gases as an alternative reaction medium for enzyme-catalyzed reactions is presented. The factors affecting the enzyme stability in supercirical fluids (effect of ...water activity, effect of pressure and temperature, number of pressurization-depressurization steps) and inhibition of anzymes are well documented with experimental results on hydrolyses. An overview of our research on various types of HP enzymatic reactors as well as expected developments in the field is presented.
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