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Biomimetic triblock copolymer membrane arrays : a stable template for functional membrane proteinsGonzález-Pérez, J. A. ...It is demonstrated that biomimetic stable triblock copolymer membrane arrays can be prepared using a scaffold containing 64 apertures of 300 mu m diameter each. The membranes were made from a stock ... solution of block copolymers with decane as a solvent using a new deposition method. By using decane, we avoid low molecular weight solvents such as chloroform and toluene, which are stron gprotein denaturants, The membranes show a low ionic conductance and a long lifetime at room temperature. Contrast phase microscopy shows the presence of a polymer region delimited by a Plateau-Gibbs border similar to what is observed in black lipid membranes. The ion-channel gramicidin A was successfully incorporated into the membrane in a functional form.Source: Langmuir. - ISSN 0743-7463 (Vol. 25, no. 18, 2009, str. 10447-10450)Type of material - article, component partPublish date - 2009Language - englishCOBISS.SI-ID - 75328513
Author
González-Pérez, J. A. |
Stibius, Karin B. |
Vissing, T |
Hélix-Nielsen, Claus |
Mouritsen, Ole G.
Topics
bilayer thickness |
gramicidin channels |
vesicles |
lipids
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Database name | Field | Year |
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González-Pérez, J. A. | ![]() |
Stibius, Karin B. | ![]() |
Vissing, T | ![]() |
Hélix-Nielsen, Claus | 36311 |
Mouritsen, Ole G. | ![]() |
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