Biomimetic triblock copolymer membrane arrays : a stable template for functional membrane proteins
González-Pérez, J. A. ...
It is demonstrated that biomimetic stable triblock copolymer membrane arrays can be prepared using a scaffold containing 64 apertures of 300 mu m diameter each. The membranes were made from a stock ...solution of block copolymers with decane as a solvent using a new deposition method. By using decane, we avoid low molecular weight solvents such as chloroform and toluene, which are stron gprotein denaturants, The membranes show a low ionic conductance and a long lifetime at room temperature. Contrast phase microscopy shows the presence of a polymer region delimited by a Plateau-Gibbs border similar to what is observed in black lipid membranes. The ion-channel gramicidin A was successfully incorporated into the membrane in a functional form.
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