Merjenje antioksidativnega potenciala in vitro z Briggs-Rauscherjevo reakcijo
Zorman, Jernej, kemik
V raziskovalni nalogi smo z Briggs-Rauscherjevo oscilirajočo reakcijo in vitro proučili antioksidativni potencial dvajsetih vzorcev hrane in pijače. Kompleksni mehanizem Briggs-Rauscherjeve reakcije ...med drugimi sestavljajo tudi procesi, v katerih sodelujejo prosti radikali, natančneje peroksidni (HOO.) in hidroksilni (OH.) radikali. Z dodajanjem vzorcev hrane in pijače v reakcijski sistem smo proučevali inhibicijski čas, antioksidanti v uporabljenih vzorcih namreč reducirajo proste radikale in s tem prekinejo oscilacije. Z merjenjem inhibicijskega časa smo tako in vitro določili antioksidativni potencial uporabljenih vzorcev pri pH-vrednosti okrog 2, kar ustreza kemijskemu okolju v človeškem želodcu.
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