Optično določevanje fosfatnih (V) ionov z evropijevim tetraciklinskim kompleksom v mikroploščah [Elektronski vir]
Turel, Matejka ...
V prispevku je predstavljen nov senzorski sistem za določevanje fosfatnih (V) ionov v mikroploščah. Kot indikator je uporabljen evropijev tetraciklinski kompleks (EuTc), ki se mu po dodatku fosfatnih ...(V) ionov (PO43-) znižuje intenziteta fluorescence. V prisotnotnosti fosfatnih ionov pokaže evropijev tetraciklinski kompleks eksitacijski vrh pri 400 nm, emisijski vrh pa pri 616 nm. Senzorski sistem je optimiran glede pH, molarnega razmerja med evropijem in tetraciklinom, časovne odzivnosti tvorjenja kompleksa med indikatorjem in PO43- ioni, linearnega območja določevanja PO43- ionov, detekcijskih mej in interferenčnih substanc.
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