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Ponovitve dinukleotidnih in trinukleotidnih motivov v genomu lipana (Thymallus thymallus, L.) = Dinucleotide and trinucleotide repeats in grayling (Tymallus thymallus, L)Sušnik Bajec, Simona ; Snoj, Aleš ; Dovč, Peter, 1958-V soškem porečju živi lipan (soški tip), ki se morfološko razlikuje od lipana v savskem porečju (savski tip). Zaradi vnosa savskega tipa lipana v soško porečje je postal soški tip lipana ena izmed ... naših najbolj ogroženih populacij rib. Uporaba genetskih markerjev bi omogočila zanesljivejšo opredelitev teh dveh fenotipsko različnih populacij na ravni genomskih razlik. Ker molekulskih markerjev, s katerimi bi lahko razlikovali med tema dvema geografsko ločenima in morfološko različnima populacijama lipana, še ni bilo na voljo, smo izdelali delno genomsko knjižnico lipana in iz nje izolirali klone z mikrosatelitskimi lokusi. Na kloniranih fragmentih genoma s skupno dolžino 1150 kb smo našli 103 dinukleotidne mikrosatelite, ob pregledu večjega števila klonov s skupno dolžino 2000 kb pa še tri trinukleotidne mikrosatelitske lokuse. Ugotovili smo, da ocena števila lokusov s ponovitvami dinukleotidnih CA in AG motivov v genomu lipana presega pogostnost pojavljanjateh motivov pri drugih vretenčarjih. Ocenjujemo, da ponovitve dinukleotidnih motivov lahko pričakujemo vsakih 11.2 kb v genomu lipana. Trinukleotidni motiv CAA je precej redkejši, v povprečju se pojavlja enkrat nasvakih 670 kb.Source: Acta biologica slovenica : ABS. - ISSN 1408-3671 (Vol. 42, št. 3, 1999, str. 23-29)Type of material - article, component partPublish date - 1999Language - slovenianCOBISS.SI-ID - 906376
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Sušnik Bajec, Simona | 15658 |
Snoj, Aleš | 11906 |
Dovč, Peter, 1958- | 05098 |
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