Scrimgeour, Jan; Kodali, Vamsi K; Kovari, Daniel T; Curtis, Jennifer E
Journal of physics. Condensed matter,
05/2010, Volume:
22, Issue:
19
Journal Article
Functional chemical micropatterns were fabricated by exploiting the photobleaching of dye-coupled species near methacrylate self-assembled monolayers. Using this approach we have demonstrated that multiple chemistries can be coupled to the monolayer using a standard fluorescence microscope. The surface bound functional groups remain active and patterns with feature sizes down to 3 µm can be readily achieved with excellent signal-to-noise ratio. Control over the ligand binding density was demonstrated to illustrate the convenient route provided by this platform for fabricating complex spatial gradients in ligand density.
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