Nanostereolithography induced by a femtosecond laser is widely used for fabrication of three-dimensional (3D) microstructures with submicron resolutions. In this study, we propose a 3D continuous laser scanning method for an effective direct writing of 3D microstructures. The advantages of the 3D continuous laser scanning method are analyzed by considering its high resolution, time economy, and fabrication effectiveness. As the 3D continuous laser scanning is an efficient manufacturing method, which can immediately respond to a complex 3D structure, it could be used for various applications including platforms for cell culture, metamaterials, and functional sensors. In this paper, the 3D open cell structure was fabricated and cells are cultured on the structure for microcell chip application. KCI Citation Count: 0