Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput

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Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput

Gierahn, Todd M; Wadsworth, 2nd, Marc H; Hughes, Travis K; Bryson, Bryan D; Butler, Andrew; Satija, Rahul; Fortune, Sarah; Love, J Christopher; Shalek, Alex K

Nature methods, 04/2017, Volume: 14, Issue: 4

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Single-cell RNA-seq can precisely resolve cellular states, but applying this method to low-input samples is challenging. Here, we present Seq-Well, a portable, low-cost platform for massively parallel single-cell RNA-seq. Barcoded mRNA capture beads and single cells are sealed in an array of subnanoliter wells using a semipermeable membrane, enabling efficient cell lysis and transcript capture. We use Seq-Well to profile thousands of primary human macrophages exposed to Mycobacterium tuberculosis.

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