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Halder, Animesh; Sarkar, Probir Kumar; Pal, Poulomi; Chakrabarti, Subhananda; Chakrabarti, Prantar; Bhattacharyya, Debasis; Chakraborty, Rajib; Pal, Samir Kumar
IEEE sensors journal, 2017-Nov.1,-1, 2017-11-1, Volume: 17, Issue: 21Journal Article
Objective of this paper: Early detection of anemia (low hemoglobin count) at point-of-care (PoC) in a low resource setting is challenging given the associated capital and recurring costs of the device. In this paper, we have developed a faster and reliable method to screen the hemoglobin level in the whole blood in a resource limited point of care setting. We have also investigated a simple scattering-based technique to predict the overall morphology of the red blood cells. Methods: In this paper, we have developed and validated a digital camera-based spectrometer for the early detection of anemia with the whole blood of 10-μL volume in a low resource PoC setting. The developed device consists of two LEDs Green (570 nm) and Red (631 nm), a web camera, a fluid sensing chamber (cuvette holder), and associate electronics. Validation of Beer-Lambert law using the blood of various degree of dilution has been achieved in this in vitro experiment. Major results: A significant number of human subjects (300) having a wide range of hemoglobin counts in a hospital is tested and found to reproduce results from a gold standard automated hematology analyzer. Our developed microcuvette requires only 10-μL unprocessed whole blood sample for the device. Conclusions: The device is expected to serve as a minimally invasive e-health care device for the anemia screening in any resource-limited point of care setting.
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