The organic material present on waste sorting units serve as a substrate for different microorganisms, increasing workers' exposure to spp. This study intends to assess the spp. contamination on Mechanical Protection Gloves (MPG) from different workstations and understand the role of MPG in workers' exposure to these genera. Sixty-seven used MPG were collected from different workstations and extracts were seeded on malt extract agar (MEA) supplemented with chloramphenicol (0.05%) and dichloran glycerol (DG18). The same extracts were used for the molecular detection of fungal species/strains, with reported toxigenic potential, namely sections ( , and ). Among spp., the sections with the highest prevalence on MEA were (88.29%) and (8.63%), whereas on DG18 were (31.79%) and (30.77%). section was detected in 22 MPG samples by RT-PCR (32.84%), in 59 samples (88.06%), in 61 samples (91.05%), and in 6 samples (8.96%). It was showed that, even with daily replacement, MPG presented spp. contamination. Thus, a more regular replacement of MPG and the adoption of complementary hygienic procedures by workers are critical to guarantee workers' protection in this occupational environment.