To explore the effect of pulse duration on the micro-morphology and mechanical properties of laser-welded glass joints, a glass welding experiment was carried out. The microstructure of the molten layer with different pulse durations was shown by field-emission scanning electron microscopy. The pores and distribution characteristics were analyzed, and the porosity variation rule of the welding layer for different pulse durations was obtained. The line-scan analysis of the joint interface was carried out with a D8-Advance type crystal X-ray diffractometer, and the relationship between the thickness of the reactive wetting layer and the pulse duration was obtained. The hardness and welding strength of the laser-welded glass joints were obtained with a Vickers hardness tester and an electronic universal testing machine. This study can provide a theoretical and experimental basis for the laser welding of glass.