The study of landscape connectivity in conservation has increased considerably since the early part of the 21st century. While the implications of landscape connectivity are self-evident for conservation, they are also important for physical geography since a proper understanding of landscape patterns and processes allows for better landscape management practices, which are at the core of geography. This paper presents a review of the literature based on 162 publications from 2000 to 2013, in which we evaluated the current state and recent advances in the integration of landscape connectivity in the identification and planning of conservation areas. The literature review and data analysis were based on a database organized into five categories: General information, study areas, research objectives, research methods in connectivity studies, and integration of connectivity with conservation. We found a substantial increase in the number of publications relating to connectivity and conservation from 2008 to 2013. Least cost analysis was the method most commonly applied. We found no implementation of landscape connectivity proposals generated by the studies (e.g. potential corridors) into real landscape elements to ensure the permanence and functionality of ecosystems. We identified four important niches for potential future research projects: a) connectivity and climate change, b) contribution of connectivity studies to restoration planning, c) connectivity and land cover/land use change modeling and planning, d) contribution of connectivity analysis in the provision of ecosystem services across landscapes.