Moonlight structures activity patterns of many nocturnal species. Bright moonlight often limits the activity of nocturnal prey, but dense vegetation weakens this effect. Using 8 years of live‐trapping data, we asked whether reintroduced megaherbivores (Bison bison) indirectly altered moonlight avoidance by small mammals in tallgrass prairies. In plots with bison, plants intercepted 20% less light, allowing more moonlight to reach ground level. During nights with no moonlight, Peromyscus maniculatus activity was similar in plots with and without bison. During nights with peak moonlight, P. maniculatus activity was four times greater in plots without bison compared to plots with bison. Conversely, Microtus ochrogaster activity was twice as great during full moons compared to new moons, but only in plots with bison. We also equipped a subset of traps with temperature sensors to estimate trap‐entry time. Although M. ochrogaster was more active on bright nights, most activity occurred before moonrise or after moonset, avoiding periods of bright moonlight. We conclude that megaherbivores play an unappreciated but important indirect role in tallgrass prairies by inducing behavioral shifts in other animal species. Because overlap in activity patterns can predict the likelihood of predator–prey encounters, such activity shifts have important implications for trophic interactions throughout restored prairie food webs. Additional work to understand interspecific and intraspecific variation in response to moonlight may improve efforts to forecast changes in community assembly due to restoration and land‐use change.