A neutron diagnostic system was developed at the Ioffe Institute as part of the Globus-M2 tokamak to optimize NBI heating conditions and evaluate heating efficiency. The system contains two compact neutron spectrometers based on the liquid organic scintillator BC-501A and two gas-discharge counters based on a 10B isotope. The BC-501A spectrometers were calibrated by measuring neutron emission produced in a 9Be(α,n)12C nuclear reaction on the cyclotron facility at the Ioffe Institute. In addition, in situ calibrations of the system, including the neutron spectrometers and the gas-discharge counters, was carried out using an Am–Be neutron source to provide accurate measurements of the total neutron yield from the plasma of the Globus-M2 tokamak. During the plasma experiments at the Globus-M2 tokamak, a deuterium beam was injected into the deuterium plasma that causes a yield of the DD-neutrons with ∼2.45 MeV energy. The neutron spectrometry diagnostic system was used to provide neutron measurements and detect the DD-neutrons in these experiments. The neutron yield and the DD-reaction rate during plasma discharges were evaluated. The energy distributions of neutrons emitted from plasma during discharges with neutron beam injection were reconstructed from the measured neutron spectra.